##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512036_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1849037 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.801661621698212 31.0 30.0 34.0 25.0 34.0 2 26.81571596458048 31.0 25.0 34.0 10.0 34.0 3 28.806991963925004 31.0 28.0 34.0 16.0 34.0 4 33.3609538370514 35.0 33.0 37.0 28.0 37.0 5 33.830341956380536 37.0 35.0 37.0 30.0 37.0 6 34.12865453747005 37.0 35.0 37.0 30.0 37.0 7 34.21574419549203 37.0 35.0 37.0 30.0 37.0 8 34.257211726969224 37.0 35.0 37.0 30.0 37.0 9 35.91594002716008 39.0 37.0 39.0 31.0 39.0 10 35.834084445038144 39.0 37.0 39.0 31.0 39.0 11 35.83846780783727 39.0 37.0 39.0 30.0 39.0 12 35.75311094369664 39.0 37.0 39.0 30.0 39.0 13 35.7550714236654 39.0 37.0 39.0 30.0 39.0 14 36.99255666598343 40.0 38.0 41.0 31.0 41.0 15 36.941086630500095 40.0 37.0 41.0 31.0 41.0 16 36.894547269740954 40.0 37.0 41.0 31.0 41.0 17 36.87445518937696 40.0 37.0 41.0 31.0 41.0 18 36.82555892607882 40.0 37.0 41.0 31.0 41.0 19 36.861401367306335 40.0 38.0 41.0 31.0 41.0 20 36.77834191527806 40.0 37.0 41.0 30.0 41.0 21 36.719706528317175 40.0 37.0 41.0 30.0 41.0 22 36.62581278795395 40.0 37.0 41.0 30.0 41.0 23 36.53622453201315 40.0 37.0 41.0 30.0 41.0 24 36.45800165167057 40.0 37.0 41.0 30.0 41.0 25 36.364369669184555 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2982073245709316 1101 2 4.198712746633802 1101 3 2.3824538637656296 1101 4 1.3499304479476777 1101 5 0.8830117576652796 1101 6 0.5895184505285584 1101 7 0.30304988122151144 1101 8 0.27537453368795894 1101 9 0.2761007156887416 1101 10 0.41389924459632965 1101 11 0.4236102308090537 1101 12 0.38949848936280773 1101 13 0.2771918233431663 1101 14 0.32265618376408156 1101 15 0.4391924759889747 1101 16 0.5547677151621144 1101 17 0.4987079294957937 1101 18 0.48285033674405753 1101 19 0.36555616469998853 1101 20 0.5183347057190062 1101 21 0.3113394856320326 1101 22 0.3672765671930307 1101 23 0.4311659483725947 1101 24 0.33045163078833184 1101 25 0.1623705728338649 1102 1 1.628862994227486 1102 2 4.37205606631111 1102 3 2.6650776252484185 1102 4 1.6435856303187748 1102 5 1.1644328264447168 1102 6 0.9695606819350076 1102 7 0.9029903241098509 1102 8 0.8404910310078861 1102 9 0.9099851027016825 1102 10 0.6769066982590957 1102 11 0.8862841646465185 1102 12 0.8913989026530658 1102 13 0.9517392795325961 1102 14 1.0021584306914733 1102 15 0.9369578209690346 1102 16 0.9780390248771482 1102 17 1.0930684124498313 1102 18 1.039246229108116 1102 19 1.0168128197560264 1102 20 1.0839435418286598 1102 21 0.9782470277280808 1102 22 0.9324962968398367 1102 23 1.1652062667324259 1102 24 1.1800850838374757 1102 25 1.1854050979110227 1103 1 1.2294246458994387 1103 2 4.074848029838421 1103 3 2.3576998798291733 1103 4 1.4205107607641025 1103 5 0.9318989799975128 1103 6 1.0028752935459408 1103 7 0.6118883427275676 1103 8 0.5893994505824907 1103 9 0.5426631757510663 1103 10 0.46852976758147236 1103 11 0.555006753791389 1103 12 0.44293737752609275 1103 13 0.5478614561061761 1103 14 0.6098998629032337 1103 15 0.7650171757630062 1103 16 0.48352171595139026 1103 17 0.5703759514100781 1103 18 0.7023484878492496 1103 19 0.7262423272707181 1103 20 0.8083310121425953 1103 21 0.8280357149539697 1103 22 0.654111565444552 1103 23 0.7026164284673087 1103 24 0.8967436030879199 1103 25 0.8951185515487552 1104 1 1.0779695740046549 1104 2 3.7865694150603204 1104 3 2.1188930551412106 1104 4 1.3675713355952084 1104 5 0.889343484422433 1104 6 0.5299895236419658 1104 7 0.38940613239238075 1104 8 0.4524901901417948 1104 9 0.40612132300859827 1104 10 0.3579914155178656 1104 11 0.1861706477240901 1104 12 0.5732319907337882 1104 13 0.473084665279913 1104 14 0.5417337933149753 1104 15 0.45482202109516123 1104 16 0.5584300276099583 1104 17 0.4967766319158784 1104 18 0.5722544275525152 1104 19 0.6447632086565918 1104 20 0.6711119252636024 1104 21 0.4318354237760076 1104 22 0.3947456136361396 1104 23 0.6357969751621084 1104 24 0.42119502707901546 1104 25 0.5879781481527715 1105 1 1.3005047852722598 1105 2 4.12077911772073 1105 3 2.2519681725121004 1105 4 1.2921096767532632 1105 5 1.0488192277714177 1105 6 0.7599504000112915 1105 7 0.6213153655692238 1105 8 0.451809438968084 1105 9 0.4552919020383328 1105 10 0.4361604296023742 1105 11 0.42315812816227094 1105 12 0.51782510497479 1105 13 0.4817309876586364 1105 14 0.503510006147529 1105 15 0.3296029287633928 1105 16 0.662201545606834 1105 17 0.6355559746388835 1105 18 0.5251417514961716 1105 19 0.4734877782966578 1105 20 0.4328490144654751 1105 21 0.5116632798010485 1105 22 0.6385327810852814 1105 23 0.36208023024818203 1105 24 0.6120087985970173 1105 25 0.6238545174798418 1106 1 0.47307557927559074 1106 2 2.339440355304788 1106 3 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-0.7302060830921135 2315 12 -0.7493674744432184 2315 13 -0.8408318797886309 2315 14 -0.9458879979036539 2315 15 -1.259734307539162 2315 16 -0.9535689202792383 2315 17 -0.9017554230193312 2315 18 -0.9016368167563797 2315 19 -0.8763785820610295 2315 20 -0.8709408180526808 2315 21 -0.9588604097399838 2315 22 -1.2470469827603807 2315 23 -1.261439605728306 2315 24 -1.2329746508372992 2315 25 -1.177700494624304 2316 1 -0.22096971108436136 2316 2 0.1709881952079897 2316 3 0.22475928552824698 2316 4 -0.15962438049300687 2316 5 -0.3353211256123956 2316 6 -0.38271164694779713 2316 7 -0.5564552683308577 2316 8 -0.7275826170399142 2316 9 -0.6478361638758514 2316 10 -0.7785373843710488 2316 11 -0.8108593505251562 2316 12 -0.6531833724267386 2316 13 -0.790518143169578 2316 14 -0.8784971853560251 2316 15 -0.6867648856862871 2316 16 -0.7274145900315929 2316 17 -0.8688132209604902 2316 18 -0.9941574341017656 2316 19 -0.7153232423253328 2316 20 -0.7709500996936853 2316 21 -0.8405675150744116 2316 22 -0.8250258225247578 2316 23 -0.9552891558545937 2316 24 -0.8326866340838421 2316 25 -0.7431110014038111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49091.0 3 2.0 4 427.0 5 2076.0 6 2403.0 7 2198.0 8 2088.0 9 1935.0 10 1996.0 11 1909.0 12 1836.0 13 1995.0 14 1955.0 15 1971.0 16 2055.0 17 2284.0 18 2702.0 19 3341.0 20 4237.0 21 5715.0 22 6737.0 23 8258.0 24 9745.0 25 11131.0 26 12799.0 27 14697.0 28 17300.0 29 21084.0 30 26785.0 31 34824.0 32 45500.0 33 62252.0 34 87326.0 35 129492.0 36 213456.0 37 297815.0 38 757620.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6412435229798 19.64449602685073 13.514602466040431 25.199657984129036 2 16.60012706579969 21.980870658445113 39.60172603313795 21.817276242617243 3 17.825386944663627 25.415662315032094 30.476675155770273 26.282275584534005 4 11.962713563871356 17.798399923852255 39.390558436634855 30.848328075641536 5 12.793037673123903 38.06862707452582 36.418741215021655 12.719594037328621 6 30.205523310525052 38.22894740827112 19.012501649508177 12.55302763169565 7 26.976258452372775 32.03846110164372 23.434360696946573 17.55091974903693 8 24.15772326769192 37.52079461946657 21.534572609727448 16.786909503114057 9 25.916733953944675 15.90779416528712 21.306496300506698 36.86897558026151 10 14.70767183963527 29.42172538648538 35.132812521125885 20.737790252753463 11 32.8006416312924 23.04372492275709 25.87179164072974 18.28384180522077 12 22.79743455647453 25.104743712537932 32.600321140139435 19.4975005908481 13 28.089162088157245 22.75719739518463 26.49411558557238 22.65952493108575 14 20.316142943597125 22.94415958144699 28.492020440910593 28.24767703404529 15 22.037255068449145 31.86702050851335 24.10059939308948 21.99512502994802 16 21.20271254712588 28.730198476287928 28.847124205735202 21.21996477085099 17 20.94490267095791 29.30709336806132 28.61759932332344 21.130404637657332 18 20.73147581784818 28.141310925896786 30.78864636520033 20.33856689105471 19 22.82847599598491 27.565677892769376 28.355337734934754 21.25050837631096 20 22.94386341782106 28.05477045752737 28.86443998083315 20.13692614381842 21 22.28139621292469 27.283578505473038 27.97693638576659 22.458088895835687 22 22.506910488420502 28.762721058560814 28.134327672078058 20.596040780940626 23 21.581413259045213 28.449332807648602 28.606010653228175 21.36324328007801 24 22.448899020299777 27.439772638159905 29.21404949068027 20.897278850860044 25 22.100993493885984 27.9992212132848 28.775952796871874 21.12383249595734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 240.0 2 398.0 3 556.0 4 556.0 5 556.0 6 1359.5 7 2163.0 8 2163.0 9 2163.0 10 3148.0 11 4133.0 12 4133.0 13 4133.0 14 7140.5 15 10148.0 16 10148.0 17 10148.0 18 17904.0 19 25660.0 20 25660.0 21 25660.0 22 40696.0 23 55732.0 24 55732.0 25 55732.0 26 78641.5 27 101551.0 28 101551.0 29 101551.0 30 129980.0 31 158409.0 32 158409.0 33 158409.0 34 178115.0 35 197821.0 36 197821.0 37 197821.0 38 209512.5 39 221204.0 40 221204.0 41 221204.0 42 229922.5 43 238641.0 44 238641.0 45 238641.0 46 238202.0 47 237763.0 48 237763.0 49 237763.0 50 224965.5 51 212168.0 52 212168.0 53 212168.0 54 185220.5 55 158273.0 56 158273.0 57 158273.0 58 132538.0 59 106803.0 60 106803.0 61 106803.0 62 85085.5 63 63368.0 64 63368.0 65 63368.0 66 48067.5 67 32767.0 68 32767.0 69 32767.0 70 23349.5 71 13932.0 72 13932.0 73 13932.0 74 9510.0 75 5088.0 76 5088.0 77 5088.0 78 3431.0 79 1774.0 80 1774.0 81 1774.0 82 1168.5 83 563.0 84 563.0 85 563.0 86 370.5 87 178.0 88 178.0 89 178.0 90 119.5 91 61.0 92 61.0 93 61.0 94 44.0 95 27.0 96 27.0 97 27.0 98 20.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4685622840429913 3 0.0 4 0.0 5 0.0 6 5.4082206034817044E-5 7 0.0 8 5.4082206034817044E-5 9 0.0 10 1.0816441206963409E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.3265764827853635E-4 19 7.030686784526216E-4 20 5.949042663829875E-4 21 0.003461261186228291 22 0.0019469594172534135 23 0.0010816441206963408 24 1.0816441206963409E-4 25 3.785754422437193E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1849037.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.85075503665404 #Duplication Level Percentage of deduplicated Percentage of total 1 87.15762681303562 67.85287054597747 2 9.462800780492927 14.733723710456273 3 1.9484940660613983 4.550752026819597 4 0.5981798916916506 1.8627502486375576 5 0.2634549559308418 1.0255083618682224 6 0.14419947445221537 0.6735622777196203 7 0.09051191386541199 0.49324945839644435 8 0.06141075724478697 0.38246990551034704 9 0.03680698422275769 0.25789063611275087 >10 0.20668521500542403 2.7616730749056595 >50 0.014446922874297555 0.7902166012397978 >100 0.012503389751220754 1.9400021948216533 >500 0.0016851719247402063 0.8815549497805868 >1k 0.0011936634466909741 1.7937760077540679 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3567 0.1929112289261924 No Hit CTGTAGGACGTGGAATATGGCAAGA 3341 0.18068865036232373 No Hit GTCCTACAGTGGACATTTCTAAATT 3296 0.17825495109075698 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3172 0.17154875754243967 No Hit CTTTAGGACGTGAAATATGGCGAGG 3145 0.1700885379794996 No Hit TATCAACGCAGAGTACTTTTTTTTT 2227 0.12044107283953756 No Hit GGTATCAACGCAGAGTACTTTTTTT 2155 0.11654715400503071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.4082206034817044E-5 7 0.0 0.0 0.0 0.0 5.4082206034817044E-5 8 0.0 0.0 0.0 0.0 5.4082206034817044E-5 9 0.0 0.0 0.0 0.0 5.4082206034817044E-5 10 0.0 0.0 0.0 0.0 5.4082206034817044E-5 11 0.0 0.0 0.0 5.4082206034817044E-5 5.4082206034817044E-5 12 0.0 0.0 0.0 5.4082206034817044E-5 1.6224661810445112E-4 13 0.0 0.0 0.0 5.4082206034817044E-5 2.704110301740852E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 45 0.009337082 13.073674 1 CGCTAGA 45 0.009337082 13.073674 1 CGGTAGG 75 1.0680056E-5 13.073674 1 GGTATCA 780 0.0 12.947966 1 TAGGACC 555 0.0 12.79099 4 GTATAAG 210 0.0 11.672923 1 TGGCGAA 85 5.525839E-5 11.135685 18 GGCGAGG 1070 0.0 10.969171 19 GGACGTG 2020 0.0 10.964791 6 GGACGGG 95 1.4167155E-5 10.959859 6 TCCGTGT 95 1.4167155E-5 10.959859 8 AGGACGT 2040 0.0 10.903692 5 GACGTGA 1160 0.0 10.852493 7 CCAACGT 70 0.0015378175 10.817522 19 GGCGAAA 70 0.0015378175 10.817522 19 TTAGGAC 1300 0.0 10.703107 3 AGGACCT 1200 0.0 10.569622 5 GTCCTAT 205 1.8189894E-12 10.522713 1 GACGTGG 930 0.0 10.48311 7 GTCCTAG 300 0.0 10.45894 1 >>END_MODULE