FastQCFastQC Report
Thu 26 May 2016
SRR1512036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849037
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45600.2466148595187657No Hit
GTCCTACAGTGGACATTTCTAAATT37550.20307868366073797No Hit
CTGTAGGACGTGGAATATGGCAAGA37460.20259194380642465No Hit
CTTTAGGACGTGAAATATGGCGAGG35690.19301939333826204No Hit
GTCCTAAAGTGTGTATTTCTCATTT34890.18869281685547667No Hit
TATCAACGCAGAGTACTTTTTTTTT29460.15932617897857101No Hit
GGTATCAACGCAGAGTACTTTTTTT27830.15051077939489582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT601.4740963E-615.8261919
TCCAACG1102.7685019E-912.94764918
GGTATCA10450.012.6097041
TAGGACC5350.011.9027744
ATAGGAC2700.011.2645363
GGCGAGG9850.011.08636619
AAGGCGT1303.2585376E-810.9634126
GACGTGG12200.010.7398917
GGACGTG23550.010.6515036
CTAGGAC2950.010.63213
AGGACGT24150.010.5867415
AGACGGA909.4999144E-510.557366
GTCTAGA1553.3669494E-910.4727161
GTGCGCC1002.4139057E-510.44415511
GTATTAG1951.4551915E-1110.2831651
TAGGACA5650.010.2614454
GTAGGAC21900.010.1554423
GTCCTAC19000.010.1014691
TAGAACG856.56585E-410.0635394
GGCGTGC856.628205E-410.0531988
Produced by FastQC (version 0.11.5)