FastQCFastQC Report
Thu 26 May 2016
SRR1512035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172530
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33130.15249501733002538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.012.3946591
GTCCGTC550.00315655212.04563911
TAGGACC3850.011.0623214
TTAGTAC1201.3439058E-711.0418363
GTAGGAC9650.010.4942393
CAGCTCG1106.3244133E-610.3248349
GTCCTAC10400.010.095491
TAGGACG9350.010.0211624
GTATAAA2950.09.9787521
AGGACGT9300.09.973275
GTCCTAA6050.09.8935341
GTCAACG1201.1589258E-59.8124392
GGACGTG9300.09.7697346
TGTAGGA10550.09.5799172
GTATAAT3400.09.5238371
GTGTAGG3200.09.50581
ATTATAC2500.09.4644313
GTATCAA18800.09.3948881
TGGTATC4100.09.3337832
CTCCTAG2950.09.3135021
Produced by FastQC (version 0.11.5)