FastQCFastQC Report
Thu 26 May 2016
SRR1512035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172530
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40360.18577418953938496No Hit
TATCAACGCAGAGTACTTTTTTTTT25320.11654614665850414No Hit
GGTATCAACGCAGAGTACTTTTTTT24390.11226542326227945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.012.4618911
TAGGACC5200.012.0637894
CCGTTTC855.355005E-511.17028315
GTTCTAG2850.011.0557411
GTAGGAC11250.010.8988413
TTGCCGC700.001498722610.85113311
TGTAGGA12100.010.68308452
TAGGACG10550.010.5408734
AGGACGT10750.010.433185
TAGGGTG1659.222276E-1010.3688765
CGTGAAA5750.010.2378079
GACGTGA5350.010.1158927
GGACGTG10750.010.0753356
CTGTAGG11900.010.0295591
GTATCAA22900.010.0067851
TGGCGAG10950.09.97187418
CATTATA1856.4210326E-109.761692
GGCGAGG5100.09.68113519
ATAGGAC2850.09.6715553
ATGGCGA11500.09.65986717
Produced by FastQC (version 0.11.5)