FastQCFastQC Report
Thu 26 May 2016
SRR1512034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574842
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75070.4766827402367984No Hit
GGTATCAACGCAGAGTACTTTTTTT43490.2761546872638652No Hit
TATCAACGCAGAGTACTTTTTTTTT43030.2732337593231575No Hit
GTCCTACAGTGGACATTTCTAAATT22930.14560190800092962No Hit
CTGTAGGACGTGGAATATGGCAAGA22880.14528441583346138No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22210.14103002078938714No Hit
GTCCTAAAGTGTGTATTTCTCATTT18180.11544015209144791No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17620.11188423981580374No Hit
GTGGTATCAACGCAGAGTACATGGG16660.10578839020041375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC350.002224936416.2251074
GCACCGT601.5249661E-615.774416
TACGCTG400.005411332514.1969695
TAGGACC4600.013.1682044
GGTATCA13100.012.6557351
GTGGTAT4750.011.772081
AGGACGT12550.011.6894045
GGCCTAA600.00462499211.4450771
GTAGGAC14200.011.3975683
TGTAGGA14350.011.2115052
GGACGTG13050.011.0964826
GTCTTAC1707.6397555E-1110.9641921
CTAAGAC1900.010.9590643
CTGTAGG14150.010.9539971
TAGGACG13600.010.9260994
GTCCTAC12100.010.8640411
GACGTGG7650.010.7637177
GTATCAA28100.010.7177591
TAGGGCA1159.2483606E-710.6991664
TTAGGAC6500.010.6295263
Produced by FastQC (version 0.11.5)