FastQCFastQC Report
Thu 26 May 2016
SRR1512034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574842
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96010.6096484599724925No Hit
GGTATCAACGCAGAGTACTTTTTTT57900.36765592992820867No Hit
TATCAACGCAGAGTACTTTTTTTTT57860.36740193619423406No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28920.1836374696636234No Hit
GTCCTACAGTGGACATTTCTAAATT28010.17785911221570164No Hit
CTGTAGGACGTGGAATATGGCAAGA26330.16719137538876916No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22190.14090302392239984No Hit
GTCCTAAAGTGTGTATTTCTCATTT21120.13410869153857974No Hit
GTGGTATCAACGCAGAGTACATGGG19530.12401244061309008No Hit
CTTTAGGACGTGAAATATGGCGAGG18900.1200120393029904No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18530.1176625972637255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.014.0712361
TAGGACC5200.012.7938954
TTGCGAG604.1103538E-412.65997818
GCGCCAC751.4871495E-512.65957513
AAGGCGT658.018459E-411.6931886
GCCGGTT658.0613024E-411.68576111
GTGGTAT5700.011.5611031
TGGTATC5100.011.5539262
GGCGAGG5200.011.14011419
AAAGGCG600.005857167811.0880415
TGTAGGA13500.011.0528422
CTGTAGG12450.010.9696241
TTACACG700.001486144510.8617554
TTAGGAC8500.010.7339713
GTCCTAA7150.010.6858471
GTAGGAC13300.010.6473793
AGGACGT13400.010.4969955
GTCCTAC12300.010.4045831
AGGACCT8600.010.3881345
GGACGTG13450.010.2424066
Produced by FastQC (version 0.11.5)