FastQCFastQC Report
Thu 26 May 2016
SRR1512033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469458
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37270.15092380595256125No Hit
GTCCTACAGTGGACATTTCTAAATT35210.14258189448858818No Hit
CTGTAGGACGTGGAATATGGCAAGA32490.13156733177887617No Hit
GTCCTAAAGTGTGTATTTCTCATTT31730.12848973337469194No Hit
CTTTAGGACGTGAAATATGGCGAGG29030.117556160096669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA500.00154173313.2516266
CGTTAGG957.1070826E-712.38439751
GGTATCA10200.011.8228491
TCCAACG1950.011.65001818
GGCGAGG9500.011.458405519
TAGGACC6800.011.4142154
AGGACGT21850.011.1765925
TATGCCG600.00603887111.0430215
GGACGTG21500.010.8302336
TCTACGT700.001538238510.8174343
TGGCGAG20550.010.64020718
TAGGACG23600.010.5884664
CGTCCAA909.8775075E-510.51716310
TTAGGAC14000.010.4793893
GGCCTAC2350.010.4301211
CCAACGA1553.8908183E-910.38166819
GACGTGA12100.010.1694887
TGTAGGA23050.010.0807912
GACGTGG11000.010.0677947
ATCCCGT2002.7284841E-119.9387210
Produced by FastQC (version 0.11.5)