##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2469458 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.435723142487138 31.0 30.0 34.0 25.0 34.0 2 26.506096074523235 31.0 25.0 34.0 10.0 34.0 3 28.46161019948507 31.0 28.0 34.0 16.0 34.0 4 32.95850344488548 35.0 33.0 37.0 27.0 37.0 5 33.412438275929375 35.0 35.0 37.0 28.0 37.0 6 33.689433470826394 37.0 35.0 37.0 29.0 37.0 7 33.757192873901886 37.0 35.0 37.0 29.0 37.0 8 33.79072006893821 37.0 35.0 37.0 29.0 37.0 9 35.41279543932312 39.0 37.0 39.0 30.0 39.0 10 35.319467267716234 39.0 35.0 39.0 30.0 39.0 11 35.32793025838058 39.0 37.0 39.0 30.0 39.0 12 35.22789899645995 39.0 35.0 39.0 29.0 39.0 13 35.22559363228692 39.0 35.0 39.0 29.0 39.0 14 36.42474178544442 40.0 37.0 41.0 29.0 41.0 15 36.37029178062554 40.0 37.0 41.0 29.0 41.0 16 36.31405676873225 40.0 37.0 41.0 29.0 41.0 17 36.280509326338006 40.0 37.0 41.0 29.0 41.0 18 36.22741872913004 40.0 37.0 41.0 28.0 41.0 19 36.25623314913637 40.0 37.0 41.0 28.0 41.0 20 36.15758721144478 40.0 37.0 41.0 27.0 41.0 21 36.099908562931624 40.0 37.0 41.0 27.0 41.0 22 35.998807835565536 40.0 37.0 41.0 27.0 41.0 23 35.909429923489284 40.0 37.0 41.0 27.0 41.0 24 35.814525697541725 40.0 36.0 41.0 27.0 41.0 25 35.719453013576256 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.938902959453678 1101 2 3.903823523647212 1101 3 2.086054864896962 1101 4 1.1289353864030858 1101 5 0.813002031959833 1101 6 0.4429663682897882 1101 7 0.1279459573351147 1101 8 0.14225553032299132 1101 9 0.1946957488484884 1101 10 0.2732139098952686 1101 11 0.2860184195898512 1101 12 -0.05411994259716835 1101 13 0.18738081825778607 1101 14 -0.11947121624793766 1101 15 0.18353288678250124 1101 16 0.2760575530258649 1101 17 0.2175710856116666 1101 18 0.26756865264017904 1101 19 0.16581717749784985 1101 20 0.11228385831460486 1101 21 0.13820926706489445 1101 22 0.30116371502468553 1101 23 0.29023272746375284 1101 24 0.16029785134124097 1101 25 0.10120875250208883 1102 1 1.8191965591054213 1102 2 4.419040695231747 1102 3 2.678852871488072 1102 4 1.896787370540494 1102 5 1.3700916931805978 1102 6 1.2656530136121447 1102 7 1.1127789233903442 1102 8 1.0469637730305479 1102 9 1.173490312654998 1102 10 0.9617246707856779 1102 11 1.0767431154016052 1102 12 0.9861696301266818 1102 13 1.190184546906515 1102 14 1.2263981186961672 1102 15 1.130408874115986 1102 16 1.2003720003857197 1102 17 1.1706514069064013 1102 18 1.3621007848984945 1102 19 1.3510037332887066 1102 20 1.397805779206962 1102 21 1.327487213427169 1102 22 1.2055317380338764 1102 23 1.3867795174212603 1102 24 1.4943888201198163 1102 25 1.454773021395816 1103 1 1.4800001168301087 1103 2 4.1640703616235335 1103 3 2.4886465888704095 1103 4 1.6824145865312303 1103 5 1.2575679795908883 1103 6 1.0817356890347156 1103 7 0.8427469083528223 1103 8 0.8185700572719696 1103 9 0.8856354593477391 1103 10 0.7877715965340641 1103 11 0.915448371767404 1103 12 0.8610393914385526 1103 13 0.8970685338592546 1103 14 0.9822249831052758 1103 15 1.1980079241419475 1103 16 1.0332935958255973 1103 17 1.0052448901587212 1103 18 0.984818974086842 1103 19 1.0509612450309689 1103 20 1.1726280650133702 1103 21 1.1548745103273035 1103 22 1.090890763811764 1103 23 1.2924766553983034 1103 24 1.3082142826870466 1103 25 1.3789148571746423 1104 1 1.2363145303980083 1104 2 3.822439683135503 1104 3 2.063462988688066 1104 4 1.5166033872457234 1104 5 1.0449330122919136 1104 6 0.8345131825456775 1104 7 0.6302501877581577 1104 8 0.6245704852227334 1104 9 0.5456347150855336 1104 10 0.6263697786736344 1104 11 0.4436276273191311 1104 12 0.6860639055748834 1104 13 0.4669955031305264 1104 14 0.6868864833518131 1104 15 0.6262848641078946 1104 16 0.7083799767575982 1104 17 0.502667510147738 1104 18 0.6198500935502267 1104 19 0.7797126521516944 1104 20 0.7287612507346992 1104 21 0.6666643678941213 1104 22 0.7726435013011823 1104 23 0.8752728484971328 1104 24 0.846546391088836 1104 25 0.8339915626979959 1105 1 1.4344977825274192 1105 2 4.130334807395027 1105 3 2.4802813139010063 1105 4 1.5301660778412582 1105 5 1.133802934125832 1105 6 0.8578648597863818 1105 7 0.8098712750728225 1105 8 0.6164391101307203 1105 9 0.6568545265054979 1105 10 0.5636045222678732 1105 11 0.6505114998698716 1105 12 0.6218254114110522 1105 13 0.5517801082691633 1105 14 0.5932288016945506 1105 15 0.4711739243623754 1105 16 0.6133377390345842 1105 17 0.7257541938730725 1105 18 0.5203211931331069 1105 19 0.5786852764988524 1105 20 0.6028647944971866 1105 21 0.5755262277560149 1105 22 0.7456053997203327 1105 23 0.6329850010436644 1105 24 0.6488587749789119 1105 25 0.8250045670094011 1106 1 0.4133076726522731 1106 2 2.242040527165745 1106 3 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0.7923261912508224 1107 15 0.8394628925944758 1107 16 0.8016544187622614 1107 17 0.8970726656629111 1107 18 0.785236967514372 1107 19 0.7275692601794219 1107 20 0.7427319565052031 1107 21 0.8746074620445299 1107 22 0.8869377728575145 1107 23 0.9873004769433251 1107 24 0.9418135473948368 1107 25 0.9263871778179293 1108 1 1.023648601647956 1108 2 3.377745713554063 1108 3 1.9133100759672033 1108 4 1.1648860913001684 1108 5 0.7460948210075244 1108 6 0.5734587816929775 1108 7 0.391830149222514 1108 8 0.2849132064952329 1108 9 0.35559576948639204 1108 10 0.36418144595523216 1108 11 0.4292456324845517 1108 12 0.34896637759554494 1108 13 0.392270787023449 1108 14 0.35649169796256075 1108 15 0.4923275812808541 1108 16 0.5555771234800204 1108 17 0.6149857981811948 1108 18 0.4814243156631264 1108 19 0.6080109344457938 1108 20 0.5947719915209859 1108 21 0.5941411030230555 1108 22 0.5594085568499025 1108 23 0.5417648748981492 1108 24 0.654972578929609 1108 25 0.4176188328164727 1109 1 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-0.9694884555061805 2315 13 -0.9488699009433574 2315 14 -1.12322021364686 2315 15 -1.5518943784759998 2315 16 -1.2432520197812025 2315 17 -1.1124586211963319 2315 18 -1.0554897109200212 2315 19 -1.2375062476619831 2315 20 -1.3323009740617096 2315 21 -1.5048677005203572 2315 22 -1.6521755185379021 2315 23 -1.6159859527840723 2315 24 -1.4409706881069937 2315 25 -1.434774478487462 2316 1 -0.4675747850949712 2316 2 0.21211886931081025 2316 3 -0.01809425969291567 2316 4 -0.4247215921979972 2316 5 -0.720562614827422 2316 6 -0.8619463810496626 2316 7 -0.8667986784187818 2316 8 -1.0122550820285667 2316 9 -1.0492576787523333 2316 10 -1.1854980903370134 2316 11 -1.2844789527280298 2316 12 -1.2087612131758405 2316 13 -1.285224506790847 2316 14 -1.5242939565864901 2316 15 -1.2801876788224433 2316 16 -1.4154583727602628 2316 17 -1.7497498400292812 2316 18 -1.5623578884010243 2316 19 -1.5652446383611576 2316 20 -1.4250989122205127 2316 21 -1.4181915343055707 2316 22 -1.6841880459578675 2316 23 -1.570151698075577 2316 24 -1.6649830638470462 2316 25 -1.5332530807780174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85672.0 3 4.0 4 725.0 5 3437.0 6 4062.0 7 3732.0 8 3479.0 9 3322.0 10 3231.0 11 3123.0 12 3134.0 13 3107.0 14 3162.0 15 3383.0 16 3419.0 17 3768.0 18 4241.0 19 5302.0 20 6942.0 21 8668.0 22 10632.0 23 12822.0 24 14921.0 25 17014.0 26 19061.0 27 21782.0 28 25064.0 29 30476.0 30 38405.0 31 49291.0 32 63443.0 33 84570.0 34 117958.0 35 171872.0 36 279930.0 37 387606.0 38 968698.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43502015422008 19.039238569759032 12.985440529865258 25.540300746155637 2 17.018994539012358 21.591266111046096 38.99696350798511 22.392775841956443 3 18.332241325829393 24.762802201940666 29.92879409165898 26.976162380570962 4 12.23608581316224 17.16862566603684 38.7947476733761 31.80054084742482 5 13.133772674003769 37.68041408276634 36.05544212535706 13.13037111787283 6 31.122603520775428 37.60824246311738 18.467468138731768 12.801685877375421 7 27.622336561302113 31.643826297106493 22.81286014987904 17.92097699171235 8 24.70555267817986 37.20238902722339 20.942093747734823 17.14996454686192 9 26.18169655041714 15.681295247783117 20.595774457391055 37.54123374440869 10 14.737630261588189 29.22293754003922 34.78635358261381 21.253078615758785 11 33.770325310250264 22.746894257768304 24.94928036840473 18.533500063576707 12 22.933007971789763 24.849541883279652 32.08732442503578 20.130125719894814 13 28.639320855021626 22.176161732655505 25.945855325338595 23.23866208698427 14 20.765892758653923 22.175999753792127 27.962775637407074 29.09533185014687 15 22.7187909249722 30.96983224659014 23.537108142758452 22.774268685679207 16 22.07010607185868 28.306940227369733 27.741107562874124 21.881846137897465 17 21.649123005938957 28.6576649612992 27.898874975804404 21.79433705695744 18 21.43372296204208 27.830261455509238 29.688290283849206 21.04772529859948 19 23.34458553794744 27.175248497433834 27.627860868973634 21.85230509564509 20 23.413822469277484 27.528394054853667 28.087993932234923 20.96978954363393 21 22.85554731688037 26.936607241566012 27.339622893654003 22.868222547899617 22 23.006869227133993 28.00460351200019 27.575555221693847 21.41297203917197 23 22.301520549015827 27.731543667645703 27.841366270935442 22.12556951240303 24 22.847240163631046 27.096269707765835 28.400321042107212 21.6561690864959 25 22.663445433959332 27.464194427256615 28.009579468771967 21.862780670012082 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 205.0 1 205.0 2 419.0 3 633.0 4 633.0 5 633.0 6 1410.0 7 2187.0 8 2187.0 9 2187.0 10 3534.0 11 4881.0 12 4881.0 13 4881.0 14 8466.0 15 12051.0 16 12051.0 17 12051.0 18 21342.5 19 30634.0 20 30634.0 21 30634.0 22 47662.0 23 64690.0 24 64690.0 25 64690.0 26 91815.5 27 118941.0 28 118941.0 29 118941.0 30 153323.5 31 187706.0 32 187706.0 33 187706.0 34 214387.0 35 241068.0 36 241068.0 37 241068.0 38 260897.0 39 280726.0 40 280726.0 41 280726.0 42 295657.0 43 310588.0 44 310588.0 45 310588.0 46 316300.5 47 322013.0 48 322013.0 49 322013.0 50 310351.0 51 298689.0 52 298689.0 53 298689.0 54 267080.5 55 235472.0 56 235472.0 57 235472.0 58 200221.0 59 164970.0 60 164970.0 61 164970.0 62 133194.5 63 101419.0 64 101419.0 65 101419.0 66 78028.5 67 54638.0 68 54638.0 69 54638.0 70 39388.0 71 24138.0 72 24138.0 73 24138.0 74 16548.5 75 8959.0 76 8959.0 77 8959.0 78 6165.0 79 3371.0 80 3371.0 81 3371.0 82 2181.5 83 992.0 84 992.0 85 992.0 86 676.5 87 361.0 88 361.0 89 361.0 90 228.0 91 95.0 92 95.0 93 95.0 94 58.5 95 22.0 96 22.0 97 22.0 98 15.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4605569319259533 3 0.0 4 0.0 5 0.0 6 8.09894316890589E-5 7 0.0 8 4.049471584452945E-5 9 0.0 10 1.619788633781178E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.264313059788828E-4 19 6.479154535124712E-4 20 6.074207376679417E-4 21 0.0030371036883397083 22 0.0015792939179366484 23 8.503890327351184E-4 24 0.0 25 4.049471584452945E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2469458.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.66654666798762 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27908281458996 59.789368111456184 2 12.120316450822552 17.6148308200895 3 3.0850902761152197 6.725485695728442 4 1.124133507646828 3.267475999778674 5 0.5013590885702844 1.8216016803506156 6 0.25674033438614735 1.1193860094135433 7 0.14937793605711167 0.7598345133163242 8 0.09960790061860093 0.579052972704147 9 0.0734206976059443 0.48017056940807024 >10 0.2826658893426667 3.4211833645523724 >50 0.015216894040976451 0.751950184072032 >100 0.010564870101029186 1.5337823608198966 >500 0.0014089186643469183 0.7024992115817191 >1k 0.0010144214383216372 1.43337850672834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3727 0.15092380595256125 No Hit GTCCTACAGTGGACATTTCTAAATT 3521 0.14258189448858818 No Hit CTGTAGGACGTGGAATATGGCAAGA 3249 0.13156733177887617 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3173 0.12848973337469194 No Hit CTTTAGGACGTGAAATATGGCGAGG 2903 0.117556160096669 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.049471584452945E-5 0.0 4 0.0 0.0 0.0 4.049471584452945E-5 0.0 5 0.0 0.0 0.0 4.049471584452945E-5 0.0 6 0.0 0.0 0.0 4.049471584452945E-5 0.0 7 0.0 0.0 0.0 4.049471584452945E-5 0.0 8 0.0 0.0 0.0 4.049471584452945E-5 0.0 9 0.0 0.0 0.0 4.049471584452945E-5 0.0 10 0.0 0.0 0.0 4.049471584452945E-5 0.0 11 0.0 0.0 0.0 4.049471584452945E-5 0.0 12 0.0 0.0 0.0 4.049471584452945E-5 4.049471584452945E-5 13 0.0 0.0 0.0 4.049471584452945E-5 8.09894316890589E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGTA 50 0.001541733 13.251626 6 CGTTAGG 95 7.1070826E-7 12.3843975 1 GGTATCA 1020 0.0 11.822849 1 TCCAACG 195 0.0 11.650018 18 GGCGAGG 950 0.0 11.4584055 19 TAGGACC 680 0.0 11.414215 4 AGGACGT 2185 0.0 11.176592 5 TATGCCG 60 0.006038871 11.043021 5 GGACGTG 2150 0.0 10.830233 6 TCTACGT 70 0.0015382385 10.817434 3 TGGCGAG 2055 0.0 10.640207 18 TAGGACG 2360 0.0 10.588466 4 CGTCCAA 90 9.8775075E-5 10.517163 10 TTAGGAC 1400 0.0 10.479389 3 GGCCTAC 235 0.0 10.430121 1 CCAACGA 155 3.8908183E-9 10.381668 19 GACGTGA 1210 0.0 10.169488 7 TGTAGGA 2305 0.0 10.080791 2 GACGTGG 1100 0.0 10.067794 7 ATCCCGT 200 2.7284841E-11 9.93872 10 >>END_MODULE