FastQCFastQC Report
Thu 26 May 2016
SRR1512033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469458
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45990.18623519816899092No Hit
GTCCTACAGTGGACATTTCTAAATT39750.16096649548200456No Hit
CTGTAGGACGTGGAATATGGCAAGA36730.14873709129695664No Hit
GTCCTAAAGTGTGTATTTCTCATTT34720.14059765341220623No Hit
CTTTAGGACGTGAAATATGGCGAGG33540.13581927694255178No Hit
TATCAACGCAGAGTACTTTTTTTTT29090.11779912839173616No Hit
GGTATCAACGCAGAGTACTTTTTTT28660.1160578556104214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.013.4445781
GGCGAGG8750.011.39573619
TAGGACC7450.011.3544044
AATCCCG2100.011.30529319
AAAGGCG1502.1173037E-910.77185
TATACCG803.7557882E-410.69259555
GGACGTG18050.010.5794896
AGGACGT18150.010.5780455
GTAGGAC19700.010.421213
GACGTGA10450.010.2674297
GTATCAA25100.010.2664431
TGGCGAG18600.010.26083518
TGTAGGA21400.010.25956252
TTAGGAC12000.010.0589613
TAGGACG19850.010.0072884
GTCCTAA10850.09.9398231
TAGGACA6750.09.8565494
ACGTGAA12200.09.6507588
TAGGACT3350.09.6463884
GTCCTAC22100.09.5871751
Produced by FastQC (version 0.11.5)