FastQCFastQC Report
Thu 26 May 2016
SRR1512032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284060
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37990.1663266288976647No Hit
GTCCTACAGTGGACATTTCTAAATT26410.11562743535633915No Hit
CTGTAGGACGTGGAATATGGCAAGA24000.10507604879031197No Hit
GTCCTAAAGTGTGTATTTCTCATTT23840.10437554179837658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG801.4043835E-613.48024752
CGGTAGG708.131869E-512.6049061
TAGGACC6250.012.1156714
GGTATCA9250.011.6585921
CCTACAC3150.011.1180523
GTATCAA19650.010.7268221
GTCCTAC17500.010.4200551
GGCCTAA1052.9042609E-510.2706641
GTAGGAC18000.010.2541493
ACTGCGC951.7063115E-49.9635468
CCCGTTT951.7063115E-49.96354612
GGCGAGG6750.09.95660119
TCCAACG1356.771461E-79.81636818
GTATTAA3800.09.8038161
CGTAGGA1104.8435853E-59.8038162
AGGACGT17200.09.7955565
GGACGTG16750.09.7196626
GACGTGG9450.09.7157757
TCTAGAC2550.09.6509643
CTACACT3750.09.5915734
Produced by FastQC (version 0.11.5)