##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512032_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2284060 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.30977776415681 31.0 30.0 34.0 23.0 34.0 2 26.385078763254906 31.0 25.0 34.0 10.0 34.0 3 28.338093132404577 31.0 27.0 34.0 16.0 34.0 4 32.81773902612015 35.0 33.0 37.0 26.0 37.0 5 33.265758342600456 35.0 35.0 37.0 28.0 37.0 6 33.54741206448167 37.0 35.0 37.0 28.0 37.0 7 33.611118797229494 37.0 35.0 37.0 28.0 37.0 8 33.649595019395285 37.0 35.0 37.0 28.0 37.0 9 35.254684640508565 39.0 35.0 39.0 29.0 39.0 10 35.163055261245326 39.0 35.0 39.0 29.0 39.0 11 35.17036373825556 39.0 35.0 39.0 29.0 39.0 12 35.06462045655543 39.0 35.0 39.0 28.0 39.0 13 35.0607646033817 39.0 35.0 39.0 28.0 39.0 14 36.25000656725305 40.0 37.0 41.0 28.0 41.0 15 36.18829321471415 40.0 36.0 41.0 27.0 41.0 16 36.13234240781766 40.0 36.0 41.0 27.0 41.0 17 36.09564591122825 40.0 36.0 41.0 27.0 41.0 18 36.040193777746644 40.0 36.0 41.0 27.0 41.0 19 36.0668625167465 40.0 37.0 41.0 27.0 41.0 20 35.96972321217481 40.0 36.0 41.0 27.0 41.0 21 35.907117151038065 40.0 36.0 41.0 27.0 41.0 22 35.803418036303775 40.0 36.0 41.0 27.0 41.0 23 35.7085711408632 40.0 36.0 41.0 27.0 41.0 24 35.610895072808944 40.0 36.0 41.0 27.0 41.0 25 35.50534530616534 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.188799562626265 1101 2 4.0782307452563344 1101 3 2.226707841160909 1101 4 1.3210578111973206 1101 5 0.9700803889890182 1101 6 0.6684390013707144 1101 7 0.34482684214426484 1101 8 0.2256532931414057 1101 9 0.38109808334461803 1101 10 0.36785868895363905 1101 11 0.538067821083537 1101 12 0.34887133609186094 1101 13 0.4466354837602253 1101 14 0.31238130047567125 1101 15 0.5122141687768718 1101 16 0.6441813771948475 1101 17 0.6680192868911021 1101 18 0.6782990117705125 1101 19 0.5474931330487465 1101 20 0.7358885375877051 1101 21 0.7344118155431829 1101 22 0.6674657418304122 1101 23 0.6156672812299675 1101 24 0.44369216321426563 1101 25 0.3675879988187205 1102 1 1.6477864907308408 1102 2 4.355309852761405 1102 3 2.595126817947026 1102 4 1.7264781402484815 1102 5 1.1726158859464206 1102 6 0.8983015210912484 1102 7 0.7023544508889117 1102 8 0.9291635478179856 1102 9 0.9234070759071642 1102 10 0.7546910080881872 1102 11 0.9733450354175446 1102 12 0.9379517958619772 1102 13 0.8990526575262834 1102 14 1.0091358643701938 1102 15 0.9768749756283484 1102 16 1.0039233199489601 1102 17 0.9118890186918662 1102 18 0.9555922279075446 1102 19 0.9444336333868151 1102 20 0.8895678253947779 1102 21 1.0344343532939106 1102 22 0.990600742957298 1102 23 1.1599708647323297 1102 24 1.0590741780891832 1102 25 1.034508215181127 1103 1 1.3756345919966613 1103 2 4.022580735604091 1103 3 2.4066194541886787 1103 4 1.6102059317676307 1103 5 1.2001471273580648 1103 6 1.0246433255757879 1103 7 0.8048024357572388 1103 8 0.6235615140950443 1103 9 0.7438962687228496 1103 10 0.6403587578982339 1103 11 0.7964370056068546 1103 12 0.777141378285954 1103 13 0.8165680559452113 1103 14 0.9150984070088626 1103 15 1.082671409338353 1103 16 0.8246307580723737 1103 17 0.9422574491039484 1103 18 0.9560368016825365 1103 19 0.8610235100377963 1103 20 1.042099066806422 1103 21 0.9102750018866388 1103 22 0.8058237573291578 1103 23 1.0625930416888636 1103 24 1.2727019809247508 1103 25 1.199732037868941 1104 1 1.5839891769335246 1104 2 4.073021738576738 1104 3 2.4743161152397697 1104 4 1.8281791212598009 1104 5 1.3719495982111738 1104 6 1.2407186824739256 1104 7 0.9535965038551026 1104 8 1.1468017525251568 1104 9 1.0853213110972604 1104 10 1.0611359998780117 1104 11 1.0305478900341924 1104 12 1.0956662078867296 1104 13 0.9923960956973019 1104 14 1.105637064319673 1104 15 1.142541684398502 1104 16 1.2657122972551775 1104 17 1.1774515213233343 1104 18 1.193392741570122 1104 19 1.224432370282102 1104 20 1.278309352067339 1104 21 1.1635973359051732 1104 22 1.2719152139269383 1104 23 1.2802815884913343 1104 24 1.130850103313378 1104 25 1.2002749197409344 1105 1 1.5997530590690907 1105 2 4.356761892228398 1105 3 2.6179407059882536 1105 4 1.7300722134183815 1105 5 1.364666542034307 1105 6 1.1678273407772792 1105 7 1.020139179468572 1105 8 0.9016900795373815 1105 9 0.8789507642399883 1105 10 0.8305734208641056 1105 11 0.8545219248489317 1105 12 0.8316146989237225 1105 13 0.903294341993778 1105 14 0.972163531952333 1105 15 0.9700920101789166 1105 16 0.9238924651698568 1105 17 1.0932405258291524 1105 18 1.0788282500144817 1105 19 1.2374506204684934 1105 20 1.0271691206173728 1105 21 1.0216016472280671 1105 22 1.1678648395225864 1105 23 0.9037507447038493 1105 24 1.1240192498011154 1105 25 1.2901587085185255 1106 1 0.5091968238646913 1106 2 2.3830420593690533 1106 3 1.2437696569105476 1106 4 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0.8738587057457394 1107 16 0.8916736973857624 1107 17 0.7731336589239035 1107 18 0.8668486914611222 1107 19 0.8149533799874007 1107 20 1.0103941102095035 1107 21 1.0399059467304 1107 22 1.1329341044020254 1107 23 0.9776577269550799 1107 24 1.1066740024756498 1107 25 0.9324675760272498 1108 1 1.362777451654214 1108 2 3.392488150346029 1108 3 2.174215141953564 1108 4 1.5874741649728676 1108 5 1.1305518116006184 1108 6 0.8997802612789343 1108 7 0.7962139927491876 1108 8 0.8011017704046495 1108 9 0.7599987925644811 1108 10 0.8772975708893895 1108 11 0.7972772495357674 1108 12 0.78337279625039 1108 13 0.7967084916868004 1108 14 0.7020871828286133 1108 15 0.7791937265574305 1108 16 0.8175960622177882 1108 17 0.7775520361610191 1108 18 0.7003474055961263 1108 19 0.7213458656311431 1108 20 0.9017208279506548 1108 21 0.8756612940579913 1108 22 1.0854882699906128 1108 23 1.0514515949554522 1108 24 0.9306863225801365 1108 25 0.9271937560152139 1109 1 1.104797890263871 1109 2 3.220840236518846 1109 3 1.9229735044101375 1109 4 1.2594276214205422 1109 5 0.7419010826558576 1109 6 0.5513172824730219 1109 7 0.4004710652228667 1109 8 0.29345546963622837 1109 9 0.47074094914359677 1109 10 0.3484174761273451 1109 11 0.3957055486096479 1109 12 0.3317720365450967 1109 13 0.22059692247662355 1109 14 0.3526119168260422 1109 15 0.49298563682020813 1109 16 0.4614509426229958 1109 17 0.4068120351399642 1109 18 0.3121958585191038 1109 19 0.4054895701897365 1109 20 0.40571815052064153 1109 21 0.41234935644335025 1109 22 0.3724270835982679 1109 23 0.5760159324333358 1109 24 0.5491303706356732 1109 25 0.5560226433811195 1110 1 0.9534768898678401 1110 2 3.5037447372609023 1110 3 1.8999802569233282 1110 4 1.1890427721720016 1110 5 0.6262482930438438 1110 6 0.47328385193230105 1110 7 0.2819865604004974 1110 8 0.2848623165565982 1110 9 0.13072210771164805 1110 10 0.15036392405738752 1110 11 0.22970593263883643 1110 12 0.12961567266143703 1110 13 0.18887801183941377 1110 14 0.2126287781075007 1110 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-0.8230807479535756 2315 14 -0.9088118130036733 2315 15 -1.2251120598600878 2315 16 -1.1059088300720816 2315 17 -1.0132931239505538 2315 18 -1.0488540748564361 2315 19 -0.9721797432344346 2315 20 -0.9844059996280379 2315 21 -0.9538493519766078 2315 22 -1.056653863215601 2315 23 -1.155444715637259 2315 24 -1.0312463000455026 2315 25 -0.9536163031588458 2316 1 -0.8079769650326156 2316 2 -0.24781283225783213 2316 3 -0.2902492743256211 2316 4 -0.5695785264978426 2316 5 -0.9659006146390396 2316 6 -1.0673820006267576 2316 7 -1.2158545405959416 2316 8 -1.2899371842137768 2316 9 -1.4199628191868712 2316 10 -1.487411173669166 2316 11 -1.5424028079165026 2316 12 -1.6171716299511019 2316 13 -1.7940854407842295 2316 14 -1.8835595498769422 2316 15 -1.5532805114236936 2316 16 -1.7687644872216097 2316 17 -1.8295898607180447 2316 18 -1.8250212768438914 2316 19 -1.735447839598109 2316 20 -1.6254501766921905 2316 21 -1.7103631630553267 2316 22 -1.8672046751929443 2316 23 -1.748576940367201 2316 24 -1.9096780622147875 2316 25 -1.6809373364713238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84109.0 3 5.0 4 706.0 5 3434.0 6 4049.0 7 3544.0 8 3342.0 9 3273.0 10 3173.0 11 2984.0 12 3012.0 13 3064.0 14 3111.0 15 3145.0 16 3299.0 17 3570.0 18 4180.0 19 5132.0 20 6700.0 21 8370.0 22 10503.0 23 12488.0 24 14316.0 25 16664.0 26 18589.0 27 21333.0 28 24325.0 29 29539.0 30 36991.0 31 46663.0 32 59855.0 33 79858.0 34 110553.0 35 159276.0 36 256994.0 37 356554.0 38 877357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78298293389841 18.883348073167955 13.026277768534978 25.307391224398657 2 17.164767602526563 21.519547580297377 38.68372566953971 22.631959147636348 3 18.472850975893802 24.602681190511632 29.709070689911826 27.21539714368274 4 12.31596367871247 16.941892945018957 38.51606350095882 32.22607987530976 5 13.387783158060646 37.463726872323846 35.83277146835022 13.315718501265291 6 31.543039425023107 37.3119409892135 18.242145445582135 12.902874140181265 7 28.00946560072853 31.468262655096623 22.5336024447694 17.988669299405444 8 25.081302592751502 36.78349080146756 20.673449909371907 17.461756696409026 9 26.225799672512984 15.741267742528656 20.463385375165277 37.569547209793086 10 14.75985744682714 29.123665753088794 34.49322697302172 21.623249827062335 11 33.972400024517746 22.751372555887322 24.656182412020698 18.62004500757423 12 23.06970920203497 24.719665858164845 31.738395663861716 20.47222927593846 13 28.602970149645806 21.958048387520467 25.94196299571815 23.497018467115574 14 21.055138656602715 21.890931061355655 27.727423973100528 29.3265063089411 15 22.85640482299064 30.54263022862797 23.570440356207804 23.030524592173585 16 22.332250466274967 28.21248128332881 27.38632084971498 22.068947400681242 17 21.89241963871352 28.466415067905398 27.607681059166573 22.033484234214512 18 21.645279218931286 27.637967645191658 29.356712856548672 21.360040279328384 19 23.382026185998964 27.150647207038393 27.518590920931945 21.948735686030705 20 23.499364505752723 27.23855749154243 28.00268295470018 21.259395048004663 21 23.104799199301578 26.78307324936975 27.159123049589184 22.95300450173949 22 23.144273814866303 27.71396985584659 27.449611978853124 21.692144350433992 23 22.543630984018684 27.47817246793185 27.68013898168247 22.298057566366992 24 22.93159805924368 26.87164686711108 28.261804209875578 21.934950863769657 25 22.76100665002664 27.23433058193534 27.86912376299829 22.135539005039732 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 349.0 3 528.0 4 528.0 5 528.0 6 1378.0 7 2228.0 8 2228.0 9 2228.0 10 3418.5 11 4609.0 12 4609.0 13 4609.0 14 7933.5 15 11258.0 16 11258.0 17 11258.0 18 19796.5 19 28335.0 20 28335.0 21 28335.0 22 43522.0 23 58709.0 24 58709.0 25 58709.0 26 82540.0 27 106371.0 28 106371.0 29 106371.0 30 135440.0 31 164509.0 32 164509.0 33 164509.0 34 189005.0 35 213501.0 36 213501.0 37 213501.0 38 233445.5 39 253390.0 40 253390.0 41 253390.0 42 268252.5 43 283115.0 44 283115.0 45 283115.0 46 289714.0 47 296313.0 48 296313.0 49 296313.0 50 288602.5 51 280892.0 52 280892.0 53 280892.0 54 252778.0 55 224664.0 56 224664.0 57 224664.0 58 192767.5 59 160871.0 60 160871.0 61 160871.0 62 130726.0 63 100581.0 64 100581.0 65 100581.0 66 77744.0 67 54907.0 68 54907.0 69 54907.0 70 39840.0 71 24773.0 72 24773.0 73 24773.0 74 17169.5 75 9566.0 76 9566.0 77 9566.0 78 6420.5 79 3275.0 80 3275.0 81 3275.0 82 2146.5 83 1018.0 84 1018.0 85 1018.0 86 678.0 87 338.0 88 338.0 89 338.0 90 219.5 91 101.0 92 101.0 93 101.0 94 64.0 95 27.0 96 27.0 97 27.0 98 19.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4522298013187043 3 0.0 4 0.0 5 0.0 6 1.3134506098789E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.378168699596333E-4 19 6.567253049394499E-4 20 4.8159855695559657E-4 21 0.0023642110977820196 22 0.0015323590448587164 23 7.8807036592734E-4 24 8.756337399192666E-5 25 3.9403518296367E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2284060.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.66505267532051 #Duplication Level Percentage of deduplicated Percentage of total 1 83.76366688851785 62.54218600509186 2 11.216483434066744 16.749586528729065 3 2.752241158356566 6.164886931916343 4 0.9890418958425623 2.9538746100473507 5 0.4498836358268359 1.6795292683387704 6 0.24244909780383883 1.0861484795164533 7 0.13621671432315566 0.7119439705138252 8 0.08986120022795063 0.5367592998789966 9 0.063328705052073 0.4255596988715579 >10 0.2725482914584773 3.3835736114327006 >50 0.01301840389961942 0.6615958017577859 >100 0.009307623641318706 1.3711638597192795 >500 0.001183606656365768 0.6214463431173527 >1k 7.693443266351182E-4 1.1117455910688763 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3799 0.1663266288976647 No Hit GTCCTACAGTGGACATTTCTAAATT 2641 0.11562743535633915 No Hit CTGTAGGACGTGGAATATGGCAAGA 2400 0.10507604879031197 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2384 0.10437554179837658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.378168699596333E-5 0.0 0.0 0.0 0.0 7 4.378168699596333E-5 0.0 0.0 4.378168699596333E-5 0.0 8 4.378168699596333E-5 0.0 0.0 4.378168699596333E-5 0.0 9 4.378168699596333E-5 0.0 0.0 8.756337399192666E-5 0.0 10 4.378168699596333E-5 0.0 0.0 8.756337399192666E-5 0.0 11 4.378168699596333E-5 0.0 0.0 8.756337399192666E-5 0.0 12 4.378168699596333E-5 0.0 0.0 8.756337399192666E-5 0.0 13 4.378168699596333E-5 0.0 0.0 1.3134506098788997E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 80 1.4043835E-6 13.4802475 2 CGGTAGG 70 8.131869E-5 12.604906 1 TAGGACC 625 0.0 12.115671 4 GGTATCA 925 0.0 11.658592 1 CCTACAC 315 0.0 11.118052 3 GTATCAA 1965 0.0 10.726822 1 GTCCTAC 1750 0.0 10.420055 1 GGCCTAA 105 2.9042609E-5 10.270664 1 GTAGGAC 1800 0.0 10.254149 3 ACTGCGC 95 1.7063115E-4 9.963546 8 CCCGTTT 95 1.7063115E-4 9.963546 12 GGCGAGG 675 0.0 9.956601 19 TCCAACG 135 6.771461E-7 9.816368 18 GTATTAA 380 0.0 9.803816 1 CGTAGGA 110 4.8435853E-5 9.803816 2 AGGACGT 1720 0.0 9.795556 5 GGACGTG 1675 0.0 9.719662 6 GACGTGG 945 0.0 9.715775 7 TCTAGAC 255 0.0 9.650964 3 CTACACT 375 0.0 9.591573 4 >>END_MODULE