FastQCFastQC Report
Thu 26 May 2016
SRR1512032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284060
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49400.21628153376005882No Hit
GTCCTACAGTGGACATTTCTAAATT30830.13497894100855495No Hit
TATCAACGCAGAGTACTTTTTTTTT30610.13401574389464374No Hit
GGTATCAACGCAGAGTACTTTTTTT30110.13182665954484557No Hit
CTGTAGGACGTGGAATATGGCAAGA27860.12197577997075384No Hit
GTCCTAAAGTGTGTATTTCTCATTT25070.10976068929888007No Hit
CTTTAGGACGTGAAATATGGCGAGG24750.10835967531500924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAATCG400.005299316314.24220215
GGTATCA11750.012.1879911
CTCGCAA658.0628926E-411.68590912
TAGGACC7800.011.4542384
GGGTCGA600.005874215611.0840626
GTCTAAG1407.734343E-910.9111541
GGACGTG15700.010.8319226
GGCGAGG6950.010.79430619
CGTGCGC803.791263E-410.68165110
TGTAGGA19500.010.6743752
AGGACGT16150.010.593345
TAGGACG16900.010.4606624
GTGGTAT5500.010.4151931
TGGCGAG16600.010.35299118
CTGTAGG19100.010.2970441
GTAGGAC18400.010.279413
GACGTGA8800.010.2500697
AGGACCT14200.010.24084955
TTAGGAC10050.010.2138773
GTCCTAC18350.010.1455891
Produced by FastQC (version 0.11.5)