FastQCFastQC Report
Thu 26 May 2016
SRR1512031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241721
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38630.17232296079663795No Hit
GGTATCAACGCAGAGTACTTTTTTT24810.11067389742077628No Hit
TATCAACGCAGAGTACTTTTTTTTT23530.1049639986421147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGA552.0223946E-413.76784717
GGTATCA12000.012.3365711
ATACCGT550.00315367212.0471346
ACACCGT600.00603782411.0432066
GCGTGCG1303.4473487E-810.9218529
GTGCGCC1408.8457455E-910.81759411
CGTGCGC1502.2664608E-910.72768710
AGGACGT13150.010.2933975
GTCCTAA7350.010.1373631
GTCCTAC12800.010.0336761
ATAGGAC2650.010.0011713
GGACGTG13100.09.9714026
AAAGGCG1901.07320375E-109.9637965
TAGGACG13400.09.9600774
AGGCGTG2107.2759576E-129.9163487
AAGTCGT1151.0720034E-59.8771537
TAGGACC5200.09.8296674
CGTTAGG800.00348225549.8038981
GCGCCAC1451.7061029E-79.79178713
GGCGAGG5800.09.79178719
Produced by FastQC (version 0.11.5)