##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512031_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2241721 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.389476656550926 31.0 30.0 34.0 25.0 34.0 2 26.462381803980065 31.0 25.0 34.0 10.0 34.0 3 28.414505194892673 31.0 28.0 34.0 16.0 34.0 4 32.909216178106014 35.0 33.0 37.0 27.0 37.0 5 33.36069832062063 35.0 35.0 37.0 28.0 37.0 6 33.639403386951365 37.0 35.0 37.0 28.0 37.0 7 33.707556381904794 37.0 35.0 37.0 28.0 37.0 8 33.74370762463304 37.0 35.0 37.0 29.0 37.0 9 35.35422650722369 39.0 37.0 39.0 30.0 39.0 10 35.27248484534873 39.0 35.0 39.0 29.0 39.0 11 35.28330956439272 39.0 35.0 39.0 29.0 39.0 12 35.178928599946204 39.0 35.0 39.0 29.0 39.0 13 35.17888800613457 39.0 35.0 39.0 29.0 39.0 14 36.37404610118744 40.0 37.0 41.0 29.0 41.0 15 36.31115825742811 40.0 37.0 41.0 28.0 41.0 16 36.25727108770449 40.0 37.0 41.0 28.0 41.0 17 36.21861284254374 40.0 37.0 41.0 28.0 41.0 18 36.166208908245046 40.0 37.0 41.0 28.0 41.0 19 36.190915818694656 40.0 37.0 41.0 27.0 41.0 20 36.09574920340221 40.0 37.0 41.0 27.0 41.0 21 36.04309189234521 40.0 37.0 41.0 27.0 41.0 22 35.93967982634771 40.0 37.0 41.0 27.0 41.0 23 35.844539084034096 40.0 36.0 41.0 27.0 41.0 24 35.74971015572411 40.0 36.0 41.0 27.0 41.0 25 35.650635828455016 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3976784321778517 1101 2 4.169454269478059 1101 3 2.4358231135644743 1101 4 1.4479491237570983 1101 5 1.1738119268059108 1101 6 0.8399157480669786 1101 7 0.4467872159241395 1101 8 0.40148318263455707 1101 9 0.4607831843986858 1101 10 0.5425547326413422 1101 11 0.6925343541129436 1101 12 0.575439828483411 1101 13 0.5929887466630888 1101 14 0.5945126132441487 1101 15 0.6520751111496565 1101 16 0.8086491783025522 1101 17 0.8598932458366306 1101 18 0.7885831519702862 1101 19 0.7457958631514359 1101 20 0.699604580772835 1101 21 0.7390078648719296 1101 22 0.7361198912528621 1101 23 0.7334200900891048 1101 24 0.8229948600934236 1101 25 0.6218543943285368 1102 1 1.6428626610548598 1102 2 4.339353164563761 1102 3 2.6139806125860936 1102 4 1.7518459589418214 1102 5 1.271042559221172 1102 6 1.0943808819975516 1102 7 1.113241180789359 1102 8 1.1012740834390229 1102 9 1.03757963864485 1102 10 0.8596146508868614 1102 11 0.9624016153576349 1102 12 1.076680013232938 1102 13 1.0403951418314534 1102 14 1.3049642665194838 1102 15 1.164903842770812 1102 16 1.098290304467291 1102 17 1.1504888158157698 1102 18 1.1210903534808168 1102 19 1.2015183558981093 1102 20 1.1788659083775315 1102 21 1.0885277559449236 1102 22 1.1201280716358326 1102 23 1.1373620318739555 1102 24 1.084783894451924 1102 25 1.0410190733931017 1103 1 1.2860276378843878 1103 2 3.9762007751941404 1103 3 2.3789751461247697 1103 4 1.458723828602288 1103 5 1.2163251120099474 1103 6 1.0150555531640677 1103 7 0.8126138469996746 1103 8 0.7313128329796257 1103 9 0.8285214411079309 1103 10 0.710889131202272 1103 11 0.6573105068964651 1103 12 0.8549439819070344 1103 13 0.8061446448909564 1103 14 0.7820394246853652 1103 15 0.9349653387227548 1103 16 0.8844543409449699 1103 17 0.9282360312573772 1103 18 0.9823562459599913 1103 19 0.8892251440890675 1103 20 1.010840466507176 1103 21 1.0667321242013514 1103 22 0.9659685325333669 1103 23 0.9413081727469077 1103 24 1.1213964402934948 1103 25 1.225220513535696 1104 1 1.410783684174742 1104 2 4.041872450011038 1104 3 2.3657394680330306 1104 4 1.7116196310495724 1104 5 1.1690438958924076 1104 6 0.9140775255934273 1104 7 0.7672315975362594 1104 8 0.846211157941454 1104 9 0.8908903920516593 1104 10 0.9103005444742394 1104 11 0.7176247148490233 1104 12 0.8083872142660269 1104 13 0.902034716362202 1104 14 1.0363146442990185 1104 15 1.0257953345185626 1104 16 1.0775891184547675 1104 17 0.9282200995865395 1104 18 1.0002355635534244 1104 19 0.9757593418630606 1104 20 0.9411973801884983 1104 21 0.8488125348844164 1104 22 0.9383036286765787 1104 23 1.1191635928340915 1104 24 1.0916949378484304 1104 25 1.1159841273696998 1105 1 1.460866019742646 1105 2 4.217453314162661 1105 3 2.53848735121214 1105 4 1.512174413418073 1105 5 1.1586793883653428 1105 6 0.89333958642613 1105 7 0.7429021277773202 1105 8 0.5554879860566615 1105 9 0.6886374784349556 1105 10 0.6835165186368073 1105 11 0.5611932977210969 1105 12 0.6712752272697315 1105 13 0.7102317267842935 1105 14 0.6909892860065057 1105 15 0.6031714327688036 1105 16 0.5987917489433556 1105 17 0.9018257600240318 1105 18 0.6777586971920186 1105 19 0.8009544505991286 1105 20 0.7488702052015128 1105 21 0.7551376063304431 1105 22 0.8376591802186439 1105 23 0.6858343674258691 1105 24 0.7714966950488034 1105 25 0.9155217733161365 1106 1 0.6081074701621105 1106 2 2.2148040344057733 1106 3 1.2241379641148171 1106 4 0.6818978136742473 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0.589033099572795 1107 17 0.5858959455537303 1107 18 0.6303894128443304 1107 19 0.6220697116519105 1107 20 0.522886153982121 1107 21 0.5878818945084134 1107 22 0.6184068065973207 1107 23 0.6585800054642093 1107 24 0.7887606882842846 1107 25 0.7404585011143823 1108 1 1.115442787490906 1108 2 3.3604195323642116 1108 3 1.9567416616031892 1108 4 1.1617217660583563 1108 5 0.8932549550592483 1108 6 0.6274047050195222 1108 7 0.40737472550775067 1108 8 0.4596931776141062 1108 9 0.47648409521497115 1108 10 0.41793337457676927 1108 11 0.46411807954743267 1108 12 0.4082464684521696 1108 13 0.4005698879271833 1108 14 0.35404206591114473 1108 15 0.43094238312450983 1108 16 0.4674478763580652 1108 17 0.496557849837167 1108 18 0.3045675172204625 1108 19 0.42488717439737655 1108 20 0.415925069793353 1108 21 0.4154923034181053 1108 22 0.6124014360989847 1108 23 0.38606192433118025 1108 24 0.31377059120478634 1108 25 0.38088958548839 1109 1 1.0638187649788406 1109 2 3.227278441339376 1109 3 1.8914942965706736 1109 4 1.2343709120901707 1109 5 0.8250036909259109 1109 6 0.6713893111669407 1109 7 0.455008499388839 1109 8 0.5508229040983323 1109 9 0.4476956738805953 1109 10 0.46828241282268124 1109 11 0.5415821448845008 1109 12 0.4982610810378034 1109 13 0.38639025439925234 1109 14 0.527311885134651 1109 15 0.4540984484326387 1109 16 0.47165297340155377 1109 17 0.49447157771030703 1109 18 0.5118023747477523 1109 19 0.5599394758796166 1109 20 0.5478371121553849 1109 21 0.6372476945217826 1109 22 0.5797068608405525 1109 23 0.6578194856208484 1109 24 0.6494205980770431 1109 25 0.5858717899633135 1110 1 1.0761763643119906 1110 2 3.728605198771998 1110 3 2.037083349085261 1110 4 1.2009657995589151 1110 5 0.7864304761440266 1110 6 0.5600458824367323 1110 7 0.42645195590453966 1110 8 0.36946697189494415 1110 9 0.3572399917466669 1110 10 0.3608914708943658 1110 11 0.3629144594163094 1110 12 0.2467745570033486 1110 13 0.3569584677918627 1110 14 0.37110877315910784 1110 15 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-1.4352168789664574 2315 16 -1.2760180423282392 2315 17 -1.3253552726737894 2315 18 -1.0728245000236214 2315 19 -1.192947367188303 2315 20 -1.263115820413816 2315 21 -1.2216475621542315 2315 22 -1.4605687153087032 2315 23 -1.4543314715794935 2315 24 -1.467406618116101 2315 25 -1.349553767895486 2316 1 -0.8072916967394512 2316 2 -0.2721692500691546 2316 3 -0.3429533948378598 2316 4 -0.6376746752856306 2316 5 -0.9753586702663739 2316 6 -1.1460507823456254 2316 7 -1.2972591803306202 2316 8 -1.2788088901740267 2316 9 -1.3032715967213946 2316 10 -1.6192102815081242 2316 11 -1.650282261895164 2316 12 -1.5544916951164964 2316 13 -1.6758482026434152 2316 14 -1.778176133567861 2316 15 -1.5936192605094988 2316 16 -1.727446968153096 2316 17 -1.8790292989699964 2316 18 -1.8917131132709528 2316 19 -1.6976087415371026 2316 20 -1.6541354185284263 2316 21 -1.6570655725734795 2316 22 -1.8464357458222196 2316 23 -1.7402109059630178 2316 24 -1.8403339314378613 2316 25 -1.7982339961266902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79388.0 3 7.0 4 683.0 5 3167.0 6 3782.0 7 3415.0 8 3187.0 9 3091.0 10 2961.0 11 2901.0 12 2913.0 13 2931.0 14 2890.0 15 3054.0 16 3226.0 17 3431.0 18 4003.0 19 4972.0 20 6401.0 21 8306.0 22 10090.0 23 11839.0 24 13713.0 25 15799.0 26 18017.0 27 20170.0 28 23271.0 29 27934.0 30 35447.0 31 45005.0 32 57411.0 33 76559.0 34 106179.0 35 155251.0 36 252586.0 37 349113.0 38 878628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.943524194134774 18.832629038136325 12.904638891280404 25.319207876448495 2 17.158199736356107 21.53479928456676 38.757916594086815 22.549084384990316 3 18.48115800315918 24.55372457143418 29.807099099308076 27.158018326098563 4 12.268966566312221 16.94483836302555 38.633442787929454 32.15275228273278 5 13.331096956311692 37.451806000835965 35.94546332929031 13.271633713562036 6 31.402494513141693 37.473948575201184 18.284843780668417 12.838713130988705 7 27.719238924023102 31.70305314532897 22.590456171842973 17.987251758804952 8 24.948800451796142 36.7726731138024 20.80546223679239 17.473064197609066 9 26.04771066515414 15.894127770583404 20.486938383500892 37.571223180761564 10 14.588766294422403 29.237174345747324 34.70842452083625 21.46563483899402 11 34.03850614706565 22.978917973698767 24.447388612315542 18.535187266920044 12 22.779819611807177 24.91679383830548 31.86069988192108 20.442686667966264 13 28.52107822516718 22.01143674881932 26.022685249413286 23.444799776600213 14 20.938966507860034 21.886051781667646 27.988330389165462 29.186651321306854 15 22.86051654063998 30.29029928345231 23.758621166505556 23.09056300940215 16 22.267623847927553 28.53910009318733 27.21016576103806 21.983110297847055 17 21.742937680469606 28.45001675052337 27.801318718966368 22.00572685004066 18 21.558558120508735 27.920835539607435 29.19825151569358 21.322354824190253 19 23.10302956765963 27.35804784391887 27.808508341414978 21.73041424700652 20 23.2052731268061 27.465421424575748 28.140398428696717 21.188907019921434 21 22.995956112782743 27.046534814679333 27.40140655007127 22.55610252246666 22 22.84969308732736 27.75587951893223 27.65332250811891 21.7411048856215 23 22.45503639645234 27.49611678983201 27.758952795688273 22.289894018027372 24 22.679717948843766 27.10350663619603 28.303120682725456 21.913654732234743 25 22.72844269399579 27.283623981860284 27.873753574836364 22.11417974930756 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 234.0 2 409.5 3 585.0 4 585.0 5 585.0 6 1425.5 7 2266.0 8 2266.0 9 2266.0 10 3522.5 11 4779.0 12 4779.0 13 4779.0 14 8089.5 15 11400.0 16 11400.0 17 11400.0 18 19945.5 19 28491.0 20 28491.0 21 28491.0 22 43624.0 23 58757.0 24 58757.0 25 58757.0 26 81737.5 27 104718.0 28 104718.0 29 104718.0 30 134350.5 31 163983.0 32 163983.0 33 163983.0 34 187946.0 35 211909.0 36 211909.0 37 211909.0 38 231409.5 39 250910.0 40 250910.0 41 250910.0 42 265984.0 43 281058.0 44 281058.0 45 281058.0 46 285847.5 47 290637.0 48 290637.0 49 290637.0 50 283057.0 51 275477.0 52 275477.0 53 275477.0 54 247078.5 55 218680.0 56 218680.0 57 218680.0 58 186930.5 59 155181.0 60 155181.0 61 155181.0 62 125508.5 63 95836.0 64 95836.0 65 95836.0 66 73466.0 67 51096.0 68 51096.0 69 51096.0 70 36812.0 71 22528.0 72 22528.0 73 22528.0 74 15632.0 75 8736.0 76 8736.0 77 8736.0 78 5895.5 79 3055.0 80 3055.0 81 3055.0 82 2008.0 83 961.0 84 961.0 85 961.0 86 630.5 87 300.0 88 300.0 89 300.0 90 198.0 91 96.0 92 96.0 93 96.0 94 66.5 95 37.0 96 37.0 97 37.0 98 24.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.454399543921835 3 0.0 4 0.0 5 0.0 6 4.4608584208293535E-5 7 0.0 8 8.921716841658707E-5 9 0.0 10 1.3382575262488062E-4 11 4.4608584208293535E-5 12 0.0 13 0.0 14 4.4608584208293535E-5 15 0.0 16 0.0 17 0.0 18 3.568686736663483E-4 19 6.69128763124403E-4 20 6.69128763124403E-4 21 0.0029441665577473737 22 0.0018289519525400352 23 8.475630999575772E-4 24 0.0 25 4.460858420829354E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2241721.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.39218885929307 #Duplication Level Percentage of deduplicated Percentage of total 1 82.3855382046895 61.28840517397766 2 12.271653402194275 18.258303150236458 3 3.0710483315622747 6.853860224327929 4 1.0643652423769987 3.1672184050470777 5 0.45475115599786664 1.6914966940487572 6 0.23402621275486393 1.0445833330370964 7 0.12827642198299263 0.6679934667247213 8 0.08299465294259846 0.4939323116813815 9 0.05928427957433777 0.3969258590233139 >10 0.22669343970695643 2.8117928903949334 >50 0.011849882340539311 0.6240055129798487 >100 0.007944361696987945 1.2481547236441735 >500 8.477604044868568E-4 0.4473234632367543 >1k 7.266517752725546E-4 1.0060047916399166 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3863 0.17232296079663795 No Hit GGTATCAACGCAGAGTACTTTTTTT 2481 0.11067389742077628 No Hit TATCAACGCAGAGTACTTTTTTTTT 2353 0.1049639986421147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGCGA 55 2.0223946E-4 13.767847 17 GGTATCA 1200 0.0 12.336571 1 ATACCGT 55 0.003153672 12.047134 6 ACACCGT 60 0.006037824 11.043206 6 GCGTGCG 130 3.4473487E-8 10.921852 9 GTGCGCC 140 8.8457455E-9 10.817594 11 CGTGCGC 150 2.2664608E-9 10.727687 10 AGGACGT 1315 0.0 10.293397 5 GTCCTAA 735 0.0 10.137363 1 GTCCTAC 1280 0.0 10.033676 1 ATAGGAC 265 0.0 10.001171 3 GGACGTG 1310 0.0 9.971402 6 AAAGGCG 190 1.07320375E-10 9.963796 5 TAGGACG 1340 0.0 9.960077 4 AGGCGTG 210 7.2759576E-12 9.916348 7 AAGTCGT 115 1.0720034E-5 9.877153 7 TAGGACC 520 0.0 9.829667 4 CGTTAGG 80 0.0034822554 9.803898 1 GCGCCAC 145 1.7061029E-7 9.791787 13 GGCGAGG 580 0.0 9.791787 19 >>END_MODULE