FastQCFastQC Report
Thu 26 May 2016
SRR1512031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241721
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48360.21572711323130755No Hit
TATCAACGCAGAGTACTTTTTTTTT30200.1347179243090465No Hit
GGTATCAACGCAGAGTACTTTTTTT30050.13404879554592208No Hit
CTGTAGGACGTGGAATATGGCAAGA25690.11459945283110609No Hit
GTCCTACAGTGGACATTTCTAAATT25610.11424258415743975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG500.001494050413.3069085
GTACCGT604.0915352E-412.6673076
GGTATCA11850.011.9233231
CTAGACG855.2969684E-511.1822764
GGACGTG13400.010.7766646
GACGTGA6550.010.7269037
TAGGACC5500.010.7146544
GTAGGAC14200.010.7097853
GTTCTAA2950.010.6793761
AGGACGT13800.010.60695655
AGAACCG909.468477E-510.5610385
TAGGACA5050.010.5401264
TGTAGGA15250.010.4710092
TAGGACG14450.010.3929394
GTATCAA24750.010.2603071
TAACACG750.002636966510.13859754
GGCGAGG6350.010.01920919
CTGTAGG16400.09.9541951
GACGTGG7950.09.7933357
TTAGGAC8550.09.7828573
Produced by FastQC (version 0.11.5)