FastQCFastQC Report
Thu 26 May 2016
SRR1512030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360446
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45060.19089612725730645No Hit
GTCCTACAGTGGACATTTCTAAATT31100.13175476160013827No Hit
CTGTAGGACGTGGAATATGGCAAGA28880.12234975932514447No Hit
GTCCTAAAGTGTGTATTTCTCATTT25410.10764914766107762No Hit
GGTATCAACGCAGAGTACTTTTTTT25320.10726786378506435No Hit
TATCAACGCAGAGTACTTTTTTTTT24750.10485306590364701No Hit
CTTTAGGACGTGAAATATGGCGAGG24130.10222644364666676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7550.013.0385464
GCGTACA708.135718E-512.6043071
GGTATCA10300.012.373161
GTAGGAC20250.011.68578153
TGTAGGA19650.011.125432
CGGTAGG1501.2696546E-911.1104641
TCCAACG1803.6379788E-1211.04329818
CTACGAC600.006038568411.0430633
AGGACGT17300.010.9427555
CTGTAGG19850.010.9145611
TAGGACG18550.010.8176954
GTGGTAT3650.010.7433981
GGACGTG17250.010.700116
TGGTATC3200.010.41605952
ATAGGAC3100.010.3814973
GACGTGG8850.010.3745977
GGCGAGG7600.010.337522519
GTCAACG951.1970998E-410.3193162
GTGTTAG2001.2732926E-1110.2935171
GTATCAA20250.010.0212021
Produced by FastQC (version 0.11.5)