FastQCFastQC Report
Thu 26 May 2016
SRR1512030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360446
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55270.2341506647472554No Hit
GTCCTACAGTGGACATTTCTAAATT35900.15208990165417893No Hit
TATCAACGCAGAGTACTTTTTTTTT33870.14348983200632423No Hit
CTGTAGGACGTGGAATATGGCAAGA33580.14226125062805928No Hit
GGTATCAACGCAGAGTACTTTTTTT31670.13416955948155562No Hit
GTCCTAAAGTGTGTATTTCTCATTT27150.11502063593066733No Hit
CTTTAGGACGTGAAATATGGCGAGG26830.11366495992706463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG7750.012.74405319
GGTATCA13300.012.2730551
TAGGACC8400.011.7653824
ACGCTGT752.0759311E-411.3992626
GCACCGT1104.975918E-711.2265456
GGACGTG15900.011.0527446
GGACCGT1053.4707828E-610.8564416
GTCTTAT1855.456968E-1210.83566951
GACGTGG7550.010.8161377
TGTAGGA19650.010.7359982
AGGACGT16950.010.7081795
CGTGCGC803.7885585E-410.682507510
GTAGGAC19100.010.4481153
TGGCGAG18350.010.40157418
GTGGTAT4500.010.3942171
TTAGGAC12050.010.3308543
CTGTAGG19800.010.317081
GTATCAA27300.010.31496051
TAGGACG18000.010.2419164
GTCCTAC19000.09.9978751
Produced by FastQC (version 0.11.5)