FastQCFastQC Report
Thu 26 May 2016
SRR1512029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400153
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32220.1342414421080656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1051.4497346E-913.5214325
GGTATCA9900.012.0869591
TAGGACC4350.011.9672454
GGACGTG10150.010.7238946
AGGACGT10750.010.653635
TTAGGAC6650.010.3901523
GTATAAG2750.010.3432671
TTCAACG951.1910567E-410.3244952
TCTATAC2400.010.2537523
TAGGACG11000.010.1533664
GTATCAA20400.010.1448291
GTAGGAC11200.010.1410733
GTCCTAC11000.09.8974371
CCAGTAC2700.09.81555753
GCCTTAC1905.8935257E-109.808271
CGTGAAA5800.09.4650039
CTAAGAC3300.09.4650023
ACCGTGC1502.795332E-79.4650028
CCCCTAG1157.841442E-59.38182451
GTACTAG1157.841442E-59.38182451
Produced by FastQC (version 0.11.5)