FastQCFastQC Report
Thu 26 May 2016
SRR1512029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400153
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41480.17282231591069402No Hit
GGTATCAACGCAGAGTACTTTTTTT25380.10574325886724721No Hit
TATCAACGCAGAGTACTTTTTTTTT25080.10449333854966746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG2800.011.5401195
TGCGCCA951.3687195E-510.99434412
GTAGGAC11850.010.7467183
GGTATCA11100.010.6660371
ATAATAC2700.010.55958753
CTAATAC1803.45608E-1110.5595863
GTCCTAC11250.010.5238241
TAGGACC4700.010.5146534
TGGCGAG10600.010.3915318
TAGGACA4950.010.1756024
TGTAGGA13300.010.146732
TATGTCG750.002659793210.12812216
AGGACGT12050.09.9374045
GTATCAA22700.09.9263721
GGACGTG11800.09.9021956
TGGTATC5400.09.8556152
TAATACA2900.09.8313394
GGCGAGG5000.09.68622819
GTCCTAA7600.09.6734521
GTGGTAT5650.09.6323181
Produced by FastQC (version 0.11.5)