FastQCFastQC Report
Thu 26 May 2016
SRR1512028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40316
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15063.7354896319079276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA14453.584184938982042No Hit
GGTATCAACGCAGAGTACTTTTTTT11542.862387141581506No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT10202.530012898104971No Hit
TATCAACGCAGAGTACTTTTTTTTT10082.5002480404802063No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5861.4535172140093262No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5411.34189899791646No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3550.8805437047326123No Hit
GTGGTATCAACGCAGAGTACATGGG3320.8234943942851474No Hit
GTACATGGGGGGTCTTGAGGGGTTT2260.5605714852663954No Hit
GGTATCAACGCAGAGTACATGGGGG2140.5308066276416311No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2030.5035221748189305No Hit
GGTATCAACGCAGAGTACATGGGGA1810.4489532691735292No Hit
GTGGTATCAACGCAGAGTACTTTTT1810.4489532691735292No Hit
ATCAACGCAGAGTACTTTTTTTTTT1640.40678638753844626No Hit
GTATCAACGCAGAGTACATGGGGAA1620.4018255779343189No Hit
GTACATGGGGAAAAAAAAAAAAAAA1610.39934517313225515No Hit
GTATCAACGCAGAGTACATGGGAAA1470.36461950590336345No Hit
GTACATGGGAAAAAAAAAAAAAAAA1470.36461950590336345No Hit
GTATCAACGCAGAGTACATGGGGGG1380.3422958626847902No Hit
GGTATCAACGCAGAGTACATGGGAA1330.3298938386744717No Hit
TATCAACGCAGAGTACATGGGAAAA1230.3050897906538347No Hit
TATCAACGCAGAGTACATGGGGAAA1150.28524655223732515No Hit
GTACTGGTTCACTATCGGTCAGTCA990.245560075404306No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA970.2405992658001786No Hit
ACGCAGAGTACATGGGAAAAAAAAA960.23811886099811488No Hit
ACGCAGAGTACATGGGGAAAAAAAA950.2356384561960512No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT910.2257168369877964No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT900.2232364321857327No Hit
GAGTACATGGGAAAAAAAAAAAAAA830.20587359857128684No Hit
GAGTACATGGGGAAAAAAAAAAAAA820.20339319376922313No Hit
CAACGCAGAGTACTTTTTTTTTTTT810.20091278896715944No Hit
GNATCAACGCAGAGTACTTTTTTTT780.19347157456096833No Hit
GGATCAACGCAGAGTACTTTTTTTT740.18354995535271357No Hit
GAATCAACGCAGAGTACTTTTTTTT720.17858914574858617No Hit
GTATCAACGCAGAGTACATGGGGGT710.17610874094652246No Hit
TATCAACGCAGAGTACATGGGGGGT690.1711479313423951No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT660.16370671693620398No Hit
TCAACGCAGAGTACTTTTTTTTTTT650.1612263121341403No Hit
ACTTAGATGTTTCAGTTCCCCCGGT610.1513046929258855No Hit
ATACAGGGTGACAGCCCCGTACACA610.1513046929258855No Hit
AGAAAAAAAAAAAAAAAAAAAAAAA600.1488242881238218No Hit
GTATCAACGCAGAGTACATGGGGAG580.14386347851969442No Hit
GNTATCAACGCAGAGTACTTTTTTT570.14138307371763073No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA560.13890266891556702No Hit
GTGGATGCCCTGGCAGTCAGAGGCG560.13890266891556702No Hit
GATATCAACGCAGAGTACTTTTTTT560.13890266891556702No Hit
GTATCAACGCAGAGTACATGGGGGA550.13642226411350333No Hit
GTACATGGGGGAAAAAAAAAAAAAA540.13394185931143965No Hit
GTACATGGGGGTTAAGCGACTAAGC540.13394185931143965No Hit
AACGCAGAGTACTTTTTTTTTTTTT530.13146145450937594No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA530.13146145450937594No Hit
GGATACCACGTGTCCCGCCCTACTC510.12650064490524854No Hit
GGTATCAACGCAGAGTACATGGGGT510.12650064490524854No Hit
ATAAAAAAAAAAAAAAAAAAAAAAA500.12402024010318485No Hit
GGTTAATGAGGCGAACCGGGGGAAC500.12402024010318485No Hit
ACGCAGAGTACATGGGGGGTCTTGA480.11905943049905744No Hit
TGTCAACGCAGAGTACTTTTTTTTT470.11657902569699374No Hit
ACGCAGAGTACATGGGGGTTAAGCG460.11409862089493004No Hit
GGTATCAACGCAGAGTACATGGGAG460.11409862089493004No Hit
GCCCAGAGCCTGAATCAGTGTGTGT440.10913781129080265No Hit
TATCAACGCAGAGTACATGGGGGTT430.10665740648873895No Hit
GTATCAACGCAGAGTACATGGGGTG430.10665740648873895No Hit
TNTCAACGCAGAGTACTTTTTTTTT420.10417700168667526No Hit
ATCATTAACTGAATCCATAGGTTAA410.10169659688461156No Hit
GCATCAACGCAGAGTACTTTTTTTT410.10169659688461156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1200.015.6162131
TGGTATC1250.014.9915662
GGTATCA3700.012.7950771
GTATCAA6750.09.0592311
TATCAAC8900.06.87076472
ATCAACG10350.06.6716053
TCAACGC10850.06.36415864
CAACGCA10950.06.2196545
AACGCAG11000.06.1053916
CATGGGA2000.0058860155.67543364
AGTACTT6850.05.661625413
GAGTACT6850.05.661625412
CGCAGAG12100.05.4721828
ACGCAGA12300.05.4601067
CAGAGTA12150.05.3718110
AGAGTAC12150.05.3718111
GCAGAGT12100.05.31583369
ACTTTTT7950.05.116219516
GTACTTT7600.05.10291314
TACTTTT7650.05.069559615
Produced by FastQC (version 0.11.5)