##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512028_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40316 Sequences flagged as poor quality 0 Sequence length 25 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.32485861692628 31.0 28.0 33.0 16.0 34.0 2 25.089021728346065 31.0 16.0 33.0 2.0 34.0 3 26.966688163508284 31.0 25.0 33.0 16.0 34.0 4 31.631486258557395 35.0 32.0 37.0 19.0 37.0 5 32.15237126699077 35.0 33.0 37.0 19.0 37.0 6 32.55642920924695 37.0 35.0 37.0 22.0 37.0 7 32.64887389621986 37.0 35.0 37.0 23.0 37.0 8 32.73876376624665 37.0 35.0 37.0 23.0 37.0 9 34.0872606409366 39.0 35.0 39.0 23.0 39.0 10 34.076570096239706 39.0 35.0 39.0 23.0 39.0 11 34.068508780632996 39.0 35.0 39.0 22.0 39.0 12 33.929779740053576 39.0 35.0 39.0 21.0 39.0 13 33.76691636075007 39.0 35.0 39.0 19.0 39.0 14 34.74756920329398 40.0 35.0 41.0 18.0 41.0 15 34.48955749578331 39.0 34.0 41.0 17.0 41.0 16 34.488168469094155 39.0 34.0 41.0 17.0 41.0 17 34.70738664550055 39.0 35.0 41.0 19.0 41.0 18 34.5895426133545 39.0 35.0 41.0 18.0 41.0 19 34.68359956344875 39.0 35.0 41.0 19.0 41.0 20 34.65767933326719 39.0 35.0 41.0 19.0 41.0 21 34.59996031352317 40.0 35.0 41.0 18.0 41.0 22 34.47866851870225 39.0 35.0 41.0 18.0 41.0 23 34.17486853854549 39.0 35.0 41.0 15.0 41.0 24 34.01150907828158 39.0 35.0 41.0 2.0 41.0 25 33.86600853259252 39.0 35.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6040428474766237 1101 2 2.5337547172547445 1101 3 1.2217628367543405 1101 4 0.010830860108846707 1101 5 -0.16378771077417298 1101 6 -0.1401750079898818 1101 7 -1.6435996919305467 1101 8 -0.7506676627270537 1101 9 -0.7764319278754996 1101 10 -0.9935512244449143 1101 11 -0.8540675245269895 1101 12 -2.4192627733892067 1101 13 -1.1547935150785946 1101 14 -1.6268453488486045 1101 15 -1.607872129513872 1101 16 -1.8617722285995626 1101 17 -2.710989049492184 1101 18 -2.501903660371852 1101 19 -2.69143875524977 1101 20 -3.65426799482249 1101 21 -3.4299017041648767 1101 22 -2.5748817978424725 1101 23 -3.3226964281753126 1101 24 -3.379827264623998 1101 25 -3.6055773679305467 1102 1 0.4706697962015376 1102 2 2.892375406909917 1102 3 1.5941766298577882 1102 4 -0.26273235828196206 1102 5 -0.6695348372109571 1102 6 -0.6631635137370111 1102 7 -0.4585422206661818 1102 8 -0.46331134088797654 1102 9 -0.23505261753067686 1102 10 0.054724637624055106 1102 11 -0.5598146509637658 1102 12 -0.42271104925127645 1102 13 -0.0823797219751512 1102 14 0.051315570691627954 1102 15 0.05994396244015121 1102 16 0.5232852426648051 1102 17 0.37866612292160795 1102 18 0.5866020867545814 1102 19 0.80856124475023 1102 20 -0.3232335120638723 1102 21 0.010328180892592798 1102 22 0.16534808721499417 1102 23 0.3497173649281322 1102 24 0.35695434457140607 1102 25 0.4266065401154293 1103 1 0.9879111755118792 1103 2 3.5475478207030235 1103 3 1.4907283539957206 1103 4 -0.46962891000610085 1103 5 -0.4971210441075087 1103 6 -0.2838531689094239 1103 7 -0.6309560137696266 1103 8 -0.015035478819008574 1103 9 -0.8557422727030897 1103 10 -0.42803398306560325 1103 11 -0.3184353406189402 1103 12 -0.5951248423547213 1103 13 -0.6685866185268736 1103 14 -0.19006373965319767 1103 15 -0.18143534790467442 1103 16 0.24742317369928912 1103 17 0.13728681257678232 1103 18 0.6555676039959621 1103 19 0.39476814130195237 1103 20 1.331938901729231 1103 21 0.9068799050305287 1103 22 -0.2484450162332763 1103 23 -0.581317117830487 1103 24 -0.9533904830148003 1103 25 -0.09063483919491233 1104 1 3.2898965464627707 1104 2 5.861027444527473 1104 3 4.482368897360402 1104 4 4.095679344957325 1104 5 3.805909258922796 1104 6 3.299218931404056 1104 7 2.929127580796724 1104 8 3.1281202160608217 1104 9 3.5538711024275287 1104 10 3.9094790785853917 1104 11 3.5519930815336167 1104 12 3.1958887417623103 1104 13 3.714903454618373 1104 14 3.1307304087271532 1104 15 3.8133399916982498 1104 16 4.123076256248922 1104 17 3.02234428384115 1104 18 2.3556720972039003 1104 19 2.6873491235381053 1104 20 2.7457320051775085 1104 21 2.5913104170472465 1104 22 2.1099666870060076 1104 23 2.3894247839458984 1104 24 2.708051523254788 1104 25 2.8550286926755106 1105 1 2.523408132915531 1105 2 5.133754717254746 1105 3 3.304115777930811 1105 4 2.4441641934421767 1105 5 1.5028789558924913 1105 6 1.8794328351473695 1105 7 1.1367924649321992 1105 8 1.6218813568807846 1105 9 1.4059210133009685 1105 10 1.7535075990845002 1105 11 1.259657965669092 1105 12 1.3277960501402077 1105 13 2.098147661391991 1105 14 1.0300173962494341 1105 15 1.9666376744076928 1105 16 1.795090516498476 1105 17 1.4831285975666404 1105 18 1.7255473200203042 1105 19 1.367384774161998 1105 20 1.1280849463539795 1105 21 1.3622551585802256 1105 22 1.134922123726156 1105 23 0.29495063064821636 1105 24 -0.30531746070243315 1105 25 -0.36244011302858326 1106 1 0.3914526480188698 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-1.9350214494775315 2314 15 -0.7669916263692187 2314 16 -0.6133445556435859 2314 17 -1.3675928230770893 2314 18 -1.8798910817555026 2314 19 -1.9650236609101484 2314 20 -1.6693623344451325 2314 21 -1.6116627104541834 2314 22 -1.5698503513015964 2314 23 -1.7472247300621078 2314 24 -1.5955505350642483 2314 25 -1.180420135226143 2315 1 -4.070709514143292 2315 2 -12.449578616078588 2315 3 -6.544903829912325 2315 4 -2.5225024732244883 2315 5 -1.1804543774408423 2315 6 -1.5068416746565525 2315 7 -1.6602663585972124 2315 8 -1.2506676627270572 2315 9 -0.7930985945421725 2315 10 -1.0435512244449114 2315 11 -2.3707341911936552 2315 12 -2.0525961067225396 2315 13 -2.4047935150785946 2315 14 -1.2768453488486031 2315 15 -2.607872129513872 2315 16 -1.3784388952662283 2315 17 -1.1443223828255213 2315 18 -1.4852369937051861 2315 19 -1.3581054219164344 2315 20 -1.8042679948224887 2315 21 -0.9965683708315396 2315 22 -1.8748817978424768 2315 23 -2.789363094841981 2315 24 -2.1131605979573322 2315 25 -1.2389107012638831 2316 1 0.9606337694387967 2316 2 2.178530836657732 2316 3 1.6456434337692656 2316 4 0.7799850889645654 2316 5 0.6819834335044348 2316 6 0.847387181064839 2316 7 0.07928588020875793 2316 8 0.7368945263276672 2316 9 0.6429710571991265 2316 10 0.42734429794314366 2316 11 0.358867798856096 2316 12 0.844916331088406 2316 13 0.3272960371602096 2316 14 0.5721596262757771 2316 15 0.801580606804535 2316 16 -0.1329165072065237 2316 17 0.12184677140333378 2316 18 -0.7402121180832992 2316 19 -0.8929312925632047 2316 20 -0.4184470993001028 2316 21 -0.07716538575691345 2316 22 -0.8624439868971976 2316 23 0.19222894495901244 2316 24 -0.10470288651454496 2316 25 -0.046373387831046387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2528.0 3 0.0 4 19.0 5 76.0 6 93.0 7 78.0 8 75.0 9 71.0 10 58.0 11 58.0 12 54.0 13 60.0 14 47.0 15 50.0 16 48.0 17 64.0 18 78.0 19 126.0 20 141.0 21 170.0 22 214.0 23 248.0 24 283.0 25 263.0 26 349.0 27 411.0 28 420.0 29 526.0 30 681.0 31 844.0 32 1163.0 33 1512.0 34 2295.0 35 3290.0 36 5751.0 37 7199.0 38 10973.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.749479114991566 20.55263418989979 16.01597380692529 13.681912888183351 2 18.659532671629446 25.012923904052936 38.11259305210918 18.214950372208435 3 19.389324337731917 33.44577835102689 30.774382379204283 16.390514932036908 4 11.119654727651552 21.710983232463537 34.98610973310844 32.18325230677647 5 9.25190991169759 42.28594106558191 32.18325230677647 16.27889671594404 6 22.904057942256177 39.470681615239606 20.61216390514932 17.013096537354897 7 21.453021133048914 40.74064887389622 21.39845222740351 16.407877765651353 8 27.988887786486753 30.17412441710487 20.69897807322155 21.138009723186823 9 29.090187518603035 16.46492707609882 21.889572378212126 32.55531302708602 10 21.071038793531105 31.51354301021927 26.056652445679134 21.358765750570495 11 27.472963587657507 29.26133544994543 21.07351919833317 22.192181764063896 12 25.612659986109733 34.070840361146935 26.870225220756026 13.446274431987302 13 29.239011806726857 27.636670304593707 27.485365611667824 15.638952277011608 14 23.454707808314318 29.643317789463243 30.34031153884314 16.561662863379304 15 25.240599265800178 29.4548070245064 31.558190296656413 13.746403413037008 16 16.41283857525548 34.46026391507094 30.819029665641434 18.307867844032145 17 17.253695803155075 32.37672388133743 29.26877666435162 21.100803651155868 18 14.63438833217581 28.499851175711875 37.58061315606707 19.28514733604524 19 17.930846314118465 29.526738763766247 39.884909217184244 12.657505704931044 20 21.105764460759996 24.384859609088203 39.80305585871614 14.706320071435657 21 22.79165529729864 26.678738868355122 37.285739091608164 13.243866742738074 22 21.694696631443172 26.678077094805776 38.125713151758696 13.501513121992359 23 20.25847100262936 28.32762811926378 38.03145309321824 13.382447784888624 24 18.550947514634387 29.40767933326719 38.01716440123028 14.024208750868143 25 17.33597916408285 30.48245070073174 38.79201289842491 13.38955723676051 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2763.0 1 2763.0 2 1709.5 3 656.0 4 656.0 5 656.0 6 753.5 7 851.0 8 851.0 9 851.0 10 715.0 11 579.0 12 579.0 13 579.0 14 434.5 15 290.0 16 290.0 17 290.0 18 588.0 19 886.0 20 886.0 21 886.0 22 1271.5 23 1657.0 24 1657.0 25 1657.0 26 2191.0 27 2725.0 28 2725.0 29 2725.0 30 3116.0 31 3507.0 32 3507.0 33 3507.0 34 3415.0 35 3323.0 36 3323.0 37 3323.0 38 2948.0 39 2573.0 40 2573.0 41 2573.0 42 2829.0 43 3085.0 44 3085.0 45 3085.0 46 3539.5 47 3994.0 48 3994.0 49 3994.0 50 4303.5 51 4613.0 52 4613.0 53 4613.0 54 4114.5 55 3616.0 56 3616.0 57 3616.0 58 2850.0 59 2084.0 60 2084.0 61 2084.0 62 1851.0 63 1618.0 64 1618.0 65 1618.0 66 1202.5 67 787.0 68 787.0 69 787.0 70 604.5 71 422.0 72 422.0 73 422.0 74 308.5 75 195.0 76 195.0 77 195.0 78 133.0 79 71.0 80 71.0 81 71.0 82 42.0 83 13.0 84 13.0 85 13.0 86 8.5 87 4.0 88 4.0 89 4.0 90 3.0 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.0380990177596985 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.00744121440619109 22 0.004960809604127394 23 0.004960809604127394 24 0.0 25 0.002480404802063697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 40316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.66097827165394 #Duplication Level Percentage of deduplicated Percentage of total 1 85.50611501431173 40.75305089790654 2 8.092635961488421 7.714058934418097 3 2.3575331772053083 3.3708701260045637 4 0.9888108248763986 1.8851076495684096 5 0.5516523549310435 1.3146145450937592 6 0.4007285974499089 1.145947018553428 7 0.23939630497007547 0.7986903462645103 8 0.20817069997397866 0.7937295366603829 9 0.22898776997137654 0.982240301617224 >10 1.1449388498568827 10.516916360750074 >50 0.16133229247983347 5.32046830042663 >100 0.08326827998959146 7.396567119753944 >500 0.010408534998698933 2.795416211925786 >1k 0.026021337496747333 15.212322651056652 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1506 3.7354896319079276 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1445 3.584184938982042 No Hit GGTATCAACGCAGAGTACTTTTTTT 1154 2.862387141581506 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 1020 2.530012898104971 No Hit TATCAACGCAGAGTACTTTTTTTTT 1008 2.5002480404802063 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 586 1.4535172140093262 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 541 1.34189899791646 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 355 0.8805437047326123 No Hit GTGGTATCAACGCAGAGTACATGGG 332 0.8234943942851474 No Hit GTACATGGGGGGTCTTGAGGGGTTT 226 0.5605714852663954 No Hit GGTATCAACGCAGAGTACATGGGGG 214 0.5308066276416311 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 203 0.5035221748189305 No Hit GGTATCAACGCAGAGTACATGGGGA 181 0.4489532691735292 No Hit GTGGTATCAACGCAGAGTACTTTTT 181 0.4489532691735292 No Hit ATCAACGCAGAGTACTTTTTTTTTT 164 0.40678638753844626 No Hit GTATCAACGCAGAGTACATGGGGAA 162 0.4018255779343189 No Hit GTACATGGGGAAAAAAAAAAAAAAA 161 0.39934517313225515 No Hit GTATCAACGCAGAGTACATGGGAAA 147 0.36461950590336345 No Hit GTACATGGGAAAAAAAAAAAAAAAA 147 0.36461950590336345 No Hit GTATCAACGCAGAGTACATGGGGGG 138 0.3422958626847902 No Hit GGTATCAACGCAGAGTACATGGGAA 133 0.3298938386744717 No Hit TATCAACGCAGAGTACATGGGAAAA 123 0.3050897906538347 No Hit TATCAACGCAGAGTACATGGGGAAA 115 0.28524655223732515 No Hit GTACTGGTTCACTATCGGTCAGTCA 99 0.245560075404306 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 97 0.2405992658001786 No Hit ACGCAGAGTACATGGGAAAAAAAAA 96 0.23811886099811488 No Hit ACGCAGAGTACATGGGGAAAAAAAA 95 0.2356384561960512 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 91 0.2257168369877964 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 90 0.2232364321857327 No Hit GAGTACATGGGAAAAAAAAAAAAAA 83 0.20587359857128684 No Hit GAGTACATGGGGAAAAAAAAAAAAA 82 0.20339319376922313 No Hit CAACGCAGAGTACTTTTTTTTTTTT 81 0.20091278896715944 No Hit GNATCAACGCAGAGTACTTTTTTTT 78 0.19347157456096833 No Hit GGATCAACGCAGAGTACTTTTTTTT 74 0.18354995535271357 No Hit GAATCAACGCAGAGTACTTTTTTTT 72 0.17858914574858617 No Hit GTATCAACGCAGAGTACATGGGGGT 71 0.17610874094652246 No Hit TATCAACGCAGAGTACATGGGGGGT 69 0.1711479313423951 No Hit TNTTTTTTTTTTTTTTTTTTTTTTT 66 0.16370671693620398 No Hit TCAACGCAGAGTACTTTTTTTTTTT 65 0.1612263121341403 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 61 0.1513046929258855 No Hit ATACAGGGTGACAGCCCCGTACACA 61 0.1513046929258855 No Hit AGAAAAAAAAAAAAAAAAAAAAAAA 60 0.1488242881238218 No Hit GTATCAACGCAGAGTACATGGGGAG 58 0.14386347851969442 No Hit GNTATCAACGCAGAGTACTTTTTTT 57 0.14138307371763073 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 56 0.13890266891556702 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 56 0.13890266891556702 No Hit GATATCAACGCAGAGTACTTTTTTT 56 0.13890266891556702 No Hit GTATCAACGCAGAGTACATGGGGGA 55 0.13642226411350333 No Hit GTACATGGGGGAAAAAAAAAAAAAA 54 0.13394185931143965 No Hit GTACATGGGGGTTAAGCGACTAAGC 54 0.13394185931143965 No Hit AACGCAGAGTACTTTTTTTTTTTTT 53 0.13146145450937594 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 53 0.13146145450937594 No Hit GGATACCACGTGTCCCGCCCTACTC 51 0.12650064490524854 No Hit GGTATCAACGCAGAGTACATGGGGT 51 0.12650064490524854 No Hit ATAAAAAAAAAAAAAAAAAAAAAAA 50 0.12402024010318485 No Hit GGTTAATGAGGCGAACCGGGGGAAC 50 0.12402024010318485 No Hit ACGCAGAGTACATGGGGGGTCTTGA 48 0.11905943049905744 No Hit TGTCAACGCAGAGTACTTTTTTTTT 47 0.11657902569699374 No Hit ACGCAGAGTACATGGGGGTTAAGCG 46 0.11409862089493004 No Hit GGTATCAACGCAGAGTACATGGGAG 46 0.11409862089493004 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 44 0.10913781129080265 No Hit TATCAACGCAGAGTACATGGGGGTT 43 0.10665740648873895 No Hit GTATCAACGCAGAGTACATGGGGTG 43 0.10665740648873895 No Hit TNTCAACGCAGAGTACTTTTTTTTT 42 0.10417700168667526 No Hit ATCATTAACTGAATCCATAGGTTAA 41 0.10169659688461156 No Hit GCATCAACGCAGAGTACTTTTTTTT 41 0.10169659688461156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.002480404802063697 0.0 12 0.0 0.0 0.0 0.002480404802063697 0.0 13 0.0 0.0 0.0 0.002480404802063697 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTAT 120 0.0 15.616213 1 TGGTATC 125 0.0 14.991566 2 GGTATCA 370 0.0 12.795077 1 GTATCAA 675 0.0 9.059231 1 TATCAAC 890 0.0 6.8707647 2 ATCAACG 1035 0.0 6.671605 3 TCAACGC 1085 0.0 6.3641586 4 CAACGCA 1095 0.0 6.219654 5 AACGCAG 1100 0.0 6.105391 6 CATGGGA 200 0.005886015 5.6754336 4 AGTACTT 685 0.0 5.6616254 13 GAGTACT 685 0.0 5.6616254 12 CGCAGAG 1210 0.0 5.472182 8 ACGCAGA 1230 0.0 5.460106 7 CAGAGTA 1215 0.0 5.37181 10 AGAGTAC 1215 0.0 5.37181 11 GCAGAGT 1210 0.0 5.3158336 9 ACTTTTT 795 0.0 5.1162195 16 GTACTTT 760 0.0 5.102913 14 TACTTTT 765 0.0 5.0695596 15 >>END_MODULE