FastQCFastQC Report
Thu 26 May 2016
SRR1512028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40316
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20054.973211628137713No Hit
GGTATCAACGCAGAGTACTTTTTTT16404.067863875384463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT14603.6213910110129977No Hit
TATCAACGCAGAGTACTTTTTTTTT14263.5370572477428315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11432.835102688758805No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7581.880146839964282No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7361.8255779343188807No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4811.1930747097926382No Hit
GTGGTATCAACGCAGAGTACATGGG3590.8904653239408672No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2890.7168369877964084No Hit
GTGGTATCAACGCAGAGTACTTTTT2840.7044349637860898No Hit
GGTATCAACGCAGAGTACATGGGGG2260.5605714852663954No Hit
GTACATGGGGGGTCTTGAGGGGTTT2060.5109633892251215No Hit
GGTATCAACGCAGAGTACATGGGGA2060.5109633892251215No Hit
ATCAACGCAGAGTACTTTTTTTTTT1820.4514336739755928No Hit
GTACATGGGGAAAAAAAAAAAAAAA1630.4043059827363826No Hit
GTATCAACGCAGAGTACATGGGGAA1610.39934517313225515No Hit
GTACATGGGAAAAAAAAAAAAAAAA1500.3720607203095545No Hit
GTATCAACGCAGAGTACATGGGGGG1390.34477626748685386No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1370.3398154578827265No Hit
GTATCAACGCAGAGTACATGGGAAA1290.31997221946621685No Hit
TATCAACGCAGAGTACATGGGAAAA1230.3050897906538347No Hit
GGTATCAACGCAGAGTACATGGGAA1210.3001289810497073No Hit
TATCAACGCAGAGTACATGGGGAAA1200.2976485762476436No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1100.27284452822700667No Hit
GTACTGGTTCACTATCGGTCAGTCA1010.2505208850084334No Hit
CAACGCAGAGTACTTTTTTTTTTTT1000.2480404802063697No Hit
ACGCAGAGTACATGGGGAAAAAAAA940.2331580513939875No Hit
ACGCAGAGTACATGGGAAAAAAAAA940.2331580513939875No Hit
GTATCAACGCAGAGTACATGGGGGT830.20587359857128684No Hit
AACGCAGAGTACTTTTTTTTTTTTT790.19595197936303205No Hit
TCAACGCAGAGTACTTTTTTTTTTT790.19595197936303205No Hit
GAGTACATGGGAAAAAAAAAAAAAA780.19347157456096833No Hit
GAGTACATGGGGAAAAAAAAAAAAA740.18354995535271357No Hit
GGATACCACGTGTCCCGCCCTACTC730.18106955055064985No Hit
ATACAGGGTGACAGCCCCGTACACA730.18106955055064985No Hit
TATCAACGCAGAGTACATGGGGGGT710.17610874094652246No Hit
GTACATGGGGGTTAAGCGACTAAGC690.1711479313423951No Hit
AGCGTACACGGTGGATGCCCTGGCA680.16866752654033138No Hit
GGTTAATGAGGCGAACCGGGGGAAC650.1612263121341403No Hit
ATCATTAACTGAATCCATAGGTTAA610.1513046929258855No Hit
ACTTAGATGTTTCAGTTCCCCCGGT600.1488242881238218No Hit
GGTATCAACGCAGAGTACATGGGAG590.14634388332175813No Hit
GTGGATGCCCTGGCAGTCAGAGGCG570.14138307371763073No Hit
GTATCAACGCAGAGTACATGGGGGA560.13890266891556702No Hit
GTATCAACGCAGAGTACATGGGGAG560.13890266891556702No Hit
ACGCAGAGTACATGGGGGGTCTTGA540.13394185931143965No Hit
GTACATGGGGGAAAAAAAAAAAAAA500.12402024010318485No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA490.12153983530112115No Hit
ACGCAGAGTACATGGGGGTTAAGCG480.11905943049905744No Hit
GTTAATGATAGTGTGTCGAAACACA480.11905943049905744No Hit
GTATTTAGCCTTGGAGGATGGTCCC480.11905943049905744No Hit
GTCTTACACGAAAAAAAAAAAAAAA450.11161821609286635No Hit
GCCCAGAGCCTGAATCAGTGTGTGT440.10913781129080265No Hit
TCATTAACCTATGGATTCAGTTAAT430.10665740648873895No Hit
CCCATATTCAGACAGGATACCACGT430.10665740648873895No Hit
CTATCGGTCAGTCAGGAGTATTTAG430.10665740648873895No Hit
GGTATCAACGCAGAGTACATGGGGT430.10665740648873895No Hit
GTGCTAATCTGCGATAAGCGTCGGT430.10665740648873895No Hit
GTCAGGAGTATTTAGCCTTGGAGGA410.10169659688461156No Hit
GTATCAACGCAGAGTACATGGGGTG410.10169659688461156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTT400.005168710414.2472110
GGGGTCT400.005168710414.247219
GGTATCA3600.013.5059671
TGGGGGG657.7428756E-411.6900176
TGGTATC1251.6239937E-811.3977662
GTGGTAT1302.7415808E-811.0003361
GTATCAA7600.09.9099511
TATCAAC10000.07.40854842
TCAACGC10400.07.12360434
ATCAACG10450.07.089523
CAACGCA10700.06.8351095
AACGCAG10750.06.8033186
ATGGGGG1700.00673272046.14585455
ACGCAGA12500.05.85085347
CGCAGAG12550.05.82754378
GCAGAGT12250.05.5050449
CAGAGTA12250.05.42750810
GAGTACT8250.05.41106112
AGAGTAC12250.05.349972211
AGTACTT8200.05.32822413
Produced by FastQC (version 0.11.5)