##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512028_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40316 Sequences flagged as poor quality 0 Sequence length 25 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.894657208056355 33.0 31.0 34.0 28.0 34.0 2 30.968970135926185 33.0 31.0 34.0 27.0 34.0 3 30.98010715348745 33.0 31.0 34.0 27.0 34.0 4 34.59934021232265 37.0 35.0 37.0 32.0 37.0 5 34.20788272646096 37.0 35.0 37.0 30.0 37.0 6 34.29095148328207 37.0 35.0 37.0 31.0 37.0 7 34.13783609485068 37.0 35.0 37.0 30.0 37.0 8 34.10010913781129 37.0 35.0 37.0 30.0 37.0 9 35.507242782022026 39.0 37.0 39.0 30.0 39.0 10 35.40834904256375 39.0 37.0 39.0 30.0 39.0 11 35.379402718523664 39.0 37.0 39.0 30.0 39.0 12 35.1884611568608 39.0 35.0 39.0 29.0 39.0 13 35.03735489631908 39.0 35.0 39.0 27.0 39.0 14 35.88848100009922 40.0 36.0 41.0 27.0 41.0 15 35.71892052783014 40.0 36.0 41.0 27.0 41.0 16 35.60988193273142 39.0 36.0 41.0 27.0 41.0 17 35.803155074908226 39.0 36.0 41.0 27.0 41.0 18 35.70951979363032 39.0 36.0 41.0 27.0 41.0 19 35.830067467010615 40.0 35.0 41.0 28.0 41.0 20 35.82594999503919 40.0 35.0 41.0 28.0 41.0 21 35.78380791745213 40.0 35.0 41.0 28.0 41.0 22 35.666459966266494 40.0 35.0 41.0 27.0 41.0 23 35.39130866157357 40.0 35.0 41.0 26.0 41.0 24 35.231967457089 39.0 35.0 41.0 25.0 41.0 25 34.958279591229285 39.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5245570168458826 1101 2 -0.11522918164617835 1101 3 0.33657055139672565 1101 4 0.43639276249441394 1101 5 -0.6094641980208806 1101 6 0.6497723649272658 1101 7 -0.21720764214012434 1101 8 0.7734286401794392 1101 9 0.7041172673974572 1101 10 1.011230217543364 1101 11 0.27660804532848005 1101 12 -0.18893062966606777 1101 13 -0.2882772008132406 1101 14 0.5377164542358486 1101 15 -1.6877802410702927 1101 16 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2 -0.18304527360020373 1103 3 0.07909928702890667 1103 4 0.7869674751380913 1103 5 -0.4772802899749067 1103 6 -1.722641428176182 1103 7 -1.2447938490366823 1103 8 -1.3599046931538936 1103 9 -0.3131241119128845 1103 10 -1.5094594376290473 1103 11 -1.4130471270853135 1103 12 -0.7280110894361798 1103 13 -1.4227599594339324 1103 14 -1.7105594078331166 1103 15 -1.328010126127758 1103 16 -1.6039135921994756 1103 17 -1.7407453291390524 1103 18 -2.416366870441692 1103 19 -0.8533243137133155 1103 20 0.05268009564127141 1103 21 -0.9011838270839689 1103 22 -1.2136687611220296 1103 23 -1.1946381093911 1103 24 -0.7184282672222366 1103 25 -0.07311221984227956 1104 1 1.0124358047246744 1104 2 1.5484071819901821 1104 3 1.4092978241239997 1104 4 1.4818473079489607 1104 5 1.829929741373057 1104 6 1.9952269103818097 1104 7 2.249459024526537 1104 8 2.185549852300653 1104 9 2.5071475704277617 1104 10 2.3536544599676077 1104 11 2.2160019847224177 1104 12 1.020160279424843 1104 13 1.5511167385806957 1104 14 2.4043831209025157 1104 15 3.3637349104448617 1104 16 2.6792630013219423 1104 17 2.3041867502757825 1104 18 2.3443436833723084 1104 19 2.86245416068585 1104 20 1.954456480176276 1104 21 2.1949499242222004 1104 22 2.2575956066940677 1104 23 2.6433974182995996 1104 24 1.688050311670139 1104 25 2.252906588935147 1105 1 -0.4617174929580372 1105 2 -0.6485625149795133 1105 3 -0.5497039584071963 1105 4 -0.9655680218193083 1105 5 -1.4427975313542163 1105 6 -0.3443452821315631 1105 7 -0.6819135244930692 1105 8 -0.5363752813891907 1105 9 -0.4135297914260718 1105 10 -0.18288742951546055 1105 11 0.39425510415200904 1105 12 -1.290891413979793 1105 13 -0.30004190669559705 1105 14 -0.08581295752885154 1105 15 -0.12111357440362269 1105 16 0.27641095141107286 1105 17 0.2622972672098314 1105 18 0.5947893161708819 1105 19 0.24302457066801963 1105 20 0.007041150408007013 1105 21 -0.1374921435139882 1105 22 0.541909332184261 1105 23 0.16033146464540948 1105 24 -0.35027410900722344 1105 25 -0.41286881213437 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-0.9555972963327335 2315 13 -0.6216105341465763 2315 14 -1.79561687909748 2315 15 -1.2044469077369584 2315 16 -0.7343733623144217 2315 17 -0.720055673966641 2315 18 -0.7238381348095118 2315 19 -0.9148185665868809 2315 20 -1.507664731944935 2315 21 -0.8914137121414356 2315 22 -1.3424043933059338 2315 23 -0.26417833927615675 2315 24 -1.1316466580268312 2315 25 -1.0167903807618188 2316 1 0.33898487754240136 2316 2 0.44845241039362094 2316 3 0.5793566210484649 2316 4 0.7174872898576012 2316 5 0.9751129164069781 2316 6 0.8378320664198 2316 7 0.6872699698001696 2316 8 0.968453515801329 2316 9 1.2638187599347717 2316 10 1.0664540981403832 2316 11 1.3512349110001196 2316 12 1.6384325544135336 2316 13 1.1938123514255636 2316 14 0.9914477975194345 2316 15 0.5206774703724975 2316 16 0.8479649461432928 2316 17 1.0590488036453038 2316 18 0.2177041537477038 2316 19 0.9361764582887417 2316 20 1.2199472083535738 2316 21 -0.3618117220916872 2316 22 0.2526204823159546 2316 23 -0.22860619997267406 2316 24 -0.3164725286736001 2316 25 -0.33868092802550365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 972.0 3 0.0 4 14.0 5 140.0 6 194.0 7 121.0 8 108.0 9 78.0 10 84.0 11 65.0 12 59.0 13 78.0 14 73.0 15 46.0 16 68.0 17 56.0 18 62.0 19 62.0 20 89.0 21 106.0 22 131.0 23 164.0 24 205.0 25 239.0 26 282.0 27 303.0 28 393.0 29 446.0 30 587.0 31 710.0 32 945.0 33 1333.0 34 1618.0 35 2327.0 36 4735.0 37 7902.0 38 15521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.62743290054078 18.92740548757694 17.434645002118273 14.010516609763998 2 16.879154678043456 23.427423355491616 41.35826966961008 18.335152296854847 3 19.64728643714654 31.654926083936896 32.83559876971922 15.862188709197342 4 11.087409465224725 20.530310546681218 36.13949796606806 32.24278202202599 5 9.03699660058063 40.3066921416342 33.97682439641696 16.679486861368204 6 22.832965233143906 38.72993026776187 20.981710797329825 17.4553937017644 7 20.780831431689652 39.550054568905644 22.581605317987897 17.087508681416807 8 27.760690544696892 29.437444190891952 21.403413037007642 21.39845222740351 9 28.075701954558983 15.07590038694315 23.204186923305883 33.64421073519198 10 20.77339021728346 30.575949995039192 27.39607103879353 21.25458874888382 11 28.169957337037406 26.91735291199524 22.331084432979463 22.581605317987897 12 25.002480404802064 32.87528524655224 28.661077487846015 13.461156860799683 13 30.102192677845025 25.315011409862088 29.030657803353506 15.552138108939378 14 23.085127492806826 27.981446572080564 32.20061514039091 16.732810794721697 15 25.193471574560967 27.755729735092764 33.38872904057942 13.662069649766842 16 15.71336442107352 31.72189701359262 33.282071634090684 19.282666931243178 17 16.576545292191685 30.44200813572775 31.83351522968548 21.14793134239508 18 14.41115189999008 25.22075602738367 40.57942256176208 19.788669510864175 19 17.320666732810793 26.989284651255087 42.963091576545295 12.726957039388829 20 20.155769421569598 22.100406786387538 43.221053675959915 14.522770116082945 21 21.807719019744024 23.806925290207364 41.544300029764855 12.841055660283759 22 20.547673380295663 23.78956245659292 42.29090187518603 13.37186228792539 23 19.332275027284453 24.89830340311539 42.37275523365413 13.396666335946025 24 17.80230683368473 26.020091777254123 42.09847451320849 14.07912687585266 25 17.02470728319111 27.11847588807303 42.4786664020639 13.378150426671958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2668.0 1 2668.0 2 1612.0 3 556.0 4 556.0 5 556.0 6 734.0 7 912.0 8 912.0 9 912.0 10 774.5 11 637.0 12 637.0 13 637.0 14 419.0 15 201.0 16 201.0 17 201.0 18 519.5 19 838.0 20 838.0 21 838.0 22 1286.5 23 1735.0 24 1735.0 25 1735.0 26 2426.0 27 3117.0 28 3117.0 29 3117.0 30 3345.0 31 3573.0 32 3573.0 33 3573.0 34 3579.5 35 3586.0 36 3586.0 37 3586.0 38 3099.0 39 2612.0 40 2612.0 41 2612.0 42 2736.5 43 2861.0 44 2861.0 45 2861.0 46 3418.5 47 3976.0 48 3976.0 49 3976.0 50 4211.5 51 4447.0 52 4447.0 53 4447.0 54 4016.0 55 3585.0 56 3585.0 57 3585.0 58 2794.0 59 2003.0 60 2003.0 61 2003.0 62 1806.0 63 1609.0 64 1609.0 65 1609.0 66 1175.0 67 741.0 68 741.0 69 741.0 70 577.5 71 414.0 72 414.0 73 414.0 74 291.0 75 168.0 76 168.0 77 168.0 78 114.0 79 60.0 80 60.0 81 60.0 82 35.0 83 10.0 84 10.0 85 10.0 86 8.0 87 6.0 88 6.0 89 6.0 90 3.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4687965075900387 2 0.0 3 0.0 4 0.0 5 0.03720607203095545 6 0.047127691239210234 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.002480404802063697 25 0.009921619208254788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 40316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.18880841353309 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80427291523087 36.19406687171347 2 9.263726165862623 8.001785891457486 3 2.5499655410062028 3.3038991963488438 4 1.2290374454399264 2.1232265105665244 5 0.6202618883528601 1.3394185931143963 6 0.36181943487250173 0.9375930151800774 7 0.2699287847461521 0.8160531798789563 8 0.2699287847461521 0.93263220557595 9 0.17803813461980245 0.6920329397757714 >10 1.1830921203767517 10.045639448357973 >50 0.1206064782908339 3.7280484175017365 >100 0.10912014702504019 9.14525250520885 >500 0.011486331265793705 3.705724774283163 >1k 0.028715828164484262 19.03462645103681 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2005 4.973211628137713 No Hit GGTATCAACGCAGAGTACTTTTTTT 1640 4.067863875384463 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 1460 3.6213910110129977 No Hit TATCAACGCAGAGTACTTTTTTTTT 1426 3.5370572477428315 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1143 2.835102688758805 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 758 1.880146839964282 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 736 1.8255779343188807 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 481 1.1930747097926382 No Hit GTGGTATCAACGCAGAGTACATGGG 359 0.8904653239408672 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 289 0.7168369877964084 No Hit GTGGTATCAACGCAGAGTACTTTTT 284 0.7044349637860898 No Hit GGTATCAACGCAGAGTACATGGGGG 226 0.5605714852663954 No Hit GTACATGGGGGGTCTTGAGGGGTTT 206 0.5109633892251215 No Hit GGTATCAACGCAGAGTACATGGGGA 206 0.5109633892251215 No Hit ATCAACGCAGAGTACTTTTTTTTTT 182 0.4514336739755928 No Hit GTACATGGGGAAAAAAAAAAAAAAA 163 0.4043059827363826 No Hit GTATCAACGCAGAGTACATGGGGAA 161 0.39934517313225515 No Hit GTACATGGGAAAAAAAAAAAAAAAA 150 0.3720607203095545 No Hit GTATCAACGCAGAGTACATGGGGGG 139 0.34477626748685386 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 137 0.3398154578827265 No Hit GTATCAACGCAGAGTACATGGGAAA 129 0.31997221946621685 No Hit TATCAACGCAGAGTACATGGGAAAA 123 0.3050897906538347 No Hit GGTATCAACGCAGAGTACATGGGAA 121 0.3001289810497073 No Hit TATCAACGCAGAGTACATGGGGAAA 120 0.2976485762476436 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 110 0.27284452822700667 No Hit GTACTGGTTCACTATCGGTCAGTCA 101 0.2505208850084334 No Hit CAACGCAGAGTACTTTTTTTTTTTT 100 0.2480404802063697 No Hit ACGCAGAGTACATGGGGAAAAAAAA 94 0.2331580513939875 No Hit ACGCAGAGTACATGGGAAAAAAAAA 94 0.2331580513939875 No Hit GTATCAACGCAGAGTACATGGGGGT 83 0.20587359857128684 No Hit AACGCAGAGTACTTTTTTTTTTTTT 79 0.19595197936303205 No Hit TCAACGCAGAGTACTTTTTTTTTTT 79 0.19595197936303205 No Hit GAGTACATGGGAAAAAAAAAAAAAA 78 0.19347157456096833 No Hit GAGTACATGGGGAAAAAAAAAAAAA 74 0.18354995535271357 No Hit GGATACCACGTGTCCCGCCCTACTC 73 0.18106955055064985 No Hit ATACAGGGTGACAGCCCCGTACACA 73 0.18106955055064985 No Hit TATCAACGCAGAGTACATGGGGGGT 71 0.17610874094652246 No Hit GTACATGGGGGTTAAGCGACTAAGC 69 0.1711479313423951 No Hit AGCGTACACGGTGGATGCCCTGGCA 68 0.16866752654033138 No Hit GGTTAATGAGGCGAACCGGGGGAAC 65 0.1612263121341403 No Hit ATCATTAACTGAATCCATAGGTTAA 61 0.1513046929258855 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 60 0.1488242881238218 No Hit GGTATCAACGCAGAGTACATGGGAG 59 0.14634388332175813 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 57 0.14138307371763073 No Hit GTATCAACGCAGAGTACATGGGGGA 56 0.13890266891556702 No Hit GTATCAACGCAGAGTACATGGGGAG 56 0.13890266891556702 No Hit ACGCAGAGTACATGGGGGGTCTTGA 54 0.13394185931143965 No Hit GTACATGGGGGAAAAAAAAAAAAAA 50 0.12402024010318485 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 49 0.12153983530112115 No Hit ACGCAGAGTACATGGGGGTTAAGCG 48 0.11905943049905744 No Hit GTTAATGATAGTGTGTCGAAACACA 48 0.11905943049905744 No Hit GTATTTAGCCTTGGAGGATGGTCCC 48 0.11905943049905744 No Hit GTCTTACACGAAAAAAAAAAAAAAA 45 0.11161821609286635 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 44 0.10913781129080265 No Hit TCATTAACCTATGGATTCAGTTAAT 43 0.10665740648873895 No Hit CCCATATTCAGACAGGATACCACGT 43 0.10665740648873895 No Hit CTATCGGTCAGTCAGGAGTATTTAG 43 0.10665740648873895 No Hit GGTATCAACGCAGAGTACATGGGGT 43 0.10665740648873895 No Hit GTGCTAATCTGCGATAAGCGTCGGT 43 0.10665740648873895 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 41 0.10169659688461156 No Hit GTATCAACGCAGAGTACATGGGGTG 41 0.10169659688461156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTCTT 40 0.0051687104 14.24721 10 GGGGTCT 40 0.0051687104 14.24721 9 GGTATCA 360 0.0 13.505967 1 TGGGGGG 65 7.7428756E-4 11.690017 6 TGGTATC 125 1.6239937E-8 11.397766 2 GTGGTAT 130 2.7415808E-8 11.000336 1 GTATCAA 760 0.0 9.909951 1 TATCAAC 1000 0.0 7.4085484 2 TCAACGC 1040 0.0 7.1236043 4 ATCAACG 1045 0.0 7.08952 3 CAACGCA 1070 0.0 6.835109 5 AACGCAG 1075 0.0 6.803318 6 ATGGGGG 170 0.0067327204 6.1458545 5 ACGCAGA 1250 0.0 5.8508534 7 CGCAGAG 1255 0.0 5.8275437 8 GCAGAGT 1225 0.0 5.505044 9 CAGAGTA 1225 0.0 5.427508 10 GAGTACT 825 0.0 5.411061 12 AGAGTAC 1225 0.0 5.3499722 11 AGTACTT 820 0.0 5.328224 13 >>END_MODULE