FastQCFastQC Report
Thu 26 May 2016
SRR1512027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463960
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43610.1769915096024286No Hit
GTCCTACAGTGGACATTTCTAAATT35240.14302180230198544No Hit
CTGTAGGACGTGGAATATGGCAAGA31480.12776181431516745No Hit
GTCCTAAAGTGTGTATTTCTCATTT28750.11668208899495121No Hit
CTTTAGGACGTGAAATATGGCGAGG25970.10539943830256986No Hit
TATCAACGCAGAGTACTTTTTTTTT25180.10219321742236075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACT400.005410387614.1977974
CGTAGGA1301.1641532E-1012.8236012
GGTATCA12000.012.5847281
ACTGCGT1001.514818E-712.3045078
CCAACGA1052.85414E-711.71905419
GCACCGT1002.0180323E-611.3582376
CGGTAGG959.569982E-611.3546431
CGTTAGG906.897735E-510.8958691
GGCGAGG8200.010.85056919
ACCGCTC700.001538574710.8171498
TAGGACC6750.010.7973374
CGTCAGA650.00841485210.5606121
CCGTAGG1501.4941179E-810.4600341
CTGCGTG1659.913492E-1010.325469
CGGTTTC1201.5976548E-610.25417213
GTATAGG3100.010.1226141
GTAGGAC20800.010.1022793
GACTGCG750.002730829410.0960067
GTTCTAG2650.09.9913071
AGGACGT20150.09.9584215
Produced by FastQC (version 0.11.5)