FastQCFastQC Report
Thu 26 May 2016
SRR1512027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463960
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54420.22086397506453026No Hit
GTCCTACAGTGGACATTTCTAAATT39690.1610821604246822No Hit
CTGTAGGACGTGGAATATGGCAAGA36360.1475673306384844No Hit
TATCAACGCAGAGTACTTTTTTTTT32370.1313738859397068No Hit
GTCCTAAAGTGTGTATTTCTCATTT31410.12747771879413627No Hit
GGTATCAACGCAGAGTACTTTTTTT30500.12378447702073087No Hit
CTTTAGGACGTGAAATATGGCGAGG29830.12106527703371808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG551.9698427E-413.8107599
GGTATCA12750.012.7293471
TAGGACC8300.011.6798044
TGTCGAG1053.489E-610.8517518
TATGTCG1053.4898476E-610.8515316
GGCGAGG8050.010.61735219
AGGACCG1451.4348188E-810.4873425
GTCCTAC19300.010.4868721
TGTAGGA21300.010.4411832
ATGTCGA1106.086131E-610.35827817
GTAGGAC21500.010.0788243
AGGACCT15300.010.0632225
GTCGAGG856.617407E-410.05525519
ACTGTTC7550.09.9350588
GTATCAA24700.09.8562261
GGACGTG18100.09.7625356
TAGGACA7900.09.7447644
CTGTAGG22650.09.6945361
AGGACGT18450.09.6844495
TCCTACA22200.09.5897772
Produced by FastQC (version 0.11.5)