FastQCFastQC Report
Thu 26 May 2016
SRR1512026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461004
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39960.16237275518446942No Hit
GTCCTACAGTGGACATTTCTAAATT29140.11840695911099698No Hit
CTGTAGGACGTGGAATATGGCAAGA27060.10995512400629986No Hit
GTCCTAAAGTGTGTATTTCTCATTT25520.10369751532301451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.013.0077621
ACCGGTG751.5360718E-512.6204397
GGCGAGG7250.011.75054219
TCGGTGA752.1464017E-411.35839513
TGCGCCC600.006039420611.04288512
GTATAAC2500.010.9808821
GCGCCAC1053.6235542E-610.8175213
GTATCAA20850.010.8153591
TAGGACC6500.010.7759134
GTACCGT803.9019575E-410.6484966
AACAGCG909.878732E-510.5170327
CGGTAGG951.19590375E-410.3203771
AGGACGT18400.010.23695855
GTCCTAC17000.010.2080671
GGACGTG17850.010.1281686
GCACTCG856.821275E-410.02211413
TGTAGGA18500.09.9633472
GAACAGT5450.09.8995196
GACGTGG9100.09.8813897
GTAGGAC18800.09.8681093
Produced by FastQC (version 0.11.5)