##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2461004 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.306972276355502 31.0 30.0 34.0 23.0 34.0 2 26.372385010345372 31.0 25.0 34.0 10.0 34.0 3 28.341610578446844 31.0 28.0 34.0 16.0 34.0 4 32.82691129311452 35.0 33.0 37.0 26.0 37.0 5 33.277014584291614 35.0 35.0 37.0 28.0 37.0 6 33.56070042958077 37.0 35.0 37.0 28.0 37.0 7 33.62419768517239 37.0 35.0 37.0 28.0 37.0 8 33.666561289620006 37.0 35.0 37.0 28.0 37.0 9 35.27648593825934 39.0 35.0 39.0 29.0 39.0 10 35.18755881745824 39.0 35.0 39.0 29.0 39.0 11 35.19335035619609 39.0 35.0 39.0 29.0 39.0 12 35.08735093482172 39.0 35.0 39.0 28.0 39.0 13 35.08552647618614 39.0 35.0 39.0 28.0 39.0 14 36.27241971162989 40.0 37.0 41.0 28.0 41.0 15 36.20647020484323 40.0 37.0 41.0 28.0 41.0 16 36.149601544735404 40.0 36.0 41.0 27.0 41.0 17 36.113120092450075 40.0 36.0 41.0 27.0 41.0 18 36.058360734074384 40.0 36.0 41.0 27.0 41.0 19 36.08680115920169 40.0 37.0 41.0 27.0 41.0 20 35.98769729752573 40.0 37.0 41.0 27.0 41.0 21 35.928642131422784 40.0 36.0 41.0 27.0 41.0 22 35.822497647301674 40.0 36.0 41.0 27.0 41.0 23 35.72510853294021 40.0 36.0 41.0 27.0 41.0 24 35.62804652085084 40.0 36.0 41.0 27.0 41.0 25 35.525338032770364 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.298373287151339 1101 2 4.208480618555161 1101 3 2.374482389338173 1101 4 1.4591788344808663 1101 5 1.108737435600645 1101 6 0.8546339345718437 1101 7 0.5037892207287555 1101 8 0.4228579826318679 1101 9 0.463450983932745 1101 10 0.6221218809490381 1101 11 0.6627050693653729 1101 12 0.6734873771542595 1101 13 0.6067395320650419 1101 14 0.6115033052756331 1101 15 0.6982561034030255 1101 16 0.6597664422547496 1101 17 0.6595058012423891 1101 18 0.7518512203552845 1101 19 0.6620680809898687 1101 20 0.6609766627729741 1101 21 0.6062712542789654 1101 22 0.7883873930999599 1101 23 0.7499282098811335 1101 24 0.7414924927142579 1101 25 0.6678843276745496 1102 1 1.670112106843888 1102 2 4.369803179045096 1102 3 2.643272809266069 1102 4 1.6818173955425664 1102 5 1.2896265717687498 1102 6 1.1184535967522535 1102 7 0.9572077277156765 1102 8 0.8935857650596262 1102 9 0.9750466516332068 1102 10 0.8636193968861932 1102 11 0.9839932591235581 1102 12 0.9354564945172683 1102 13 1.086868649071711 1102 14 1.0821924165808454 1102 15 1.083639552891455 1102 16 1.038751946860259 1102 17 1.1222626756924399 1102 18 1.1162488032279043 1102 19 1.155407131980276 1102 20 1.1183292984630597 1102 21 1.1798403316345727 1102 22 1.0998583818618428 1102 23 1.197976897431829 1102 24 1.1246919348782498 1102 25 1.0680423731104867 1103 1 1.3535244004187703 1103 2 4.1656189623333475 1103 3 2.4329702009093204 1103 4 1.5513875243290585 1103 5 1.157356117236091 1103 6 0.8513211587955212 1103 7 0.7370593669566858 1103 8 0.6862665592335802 1103 9 0.7798271994838117 1103 10 0.5999880073224588 1103 11 0.6528905065169397 1103 12 0.628908439338197 1103 13 0.6774076665072712 1103 14 0.6835037860986617 1103 15 0.8751297809914149 1103 16 0.6983971721449009 1103 17 0.9011842215805714 1103 18 0.9387828379095993 1103 19 0.9104424365150052 1103 20 0.9010457494504251 1103 21 0.9533620179295355 1103 22 0.9649569975716119 1103 23 1.0590106247673532 1103 24 1.1470166985555394 1103 25 1.1014050080075037 1104 1 1.4219934892461161 1104 2 3.9653881639241995 1104 3 2.316950594287718 1104 4 1.6348931608457065 1104 5 1.2034535008203946 1104 6 0.9557636134643275 1104 7 0.7001279352066234 1104 8 0.7708143089951704 1104 9 0.8445712956192182 1104 10 0.8004291083868011 1104 11 0.8114102338644287 1104 12 0.764361965031803 1104 13 0.792451697358544 1104 14 0.7731264467350556 1104 15 0.7251495210565935 1104 16 0.8920966078112755 1104 17 0.8526367475532268 1104 18 0.9905700049872266 1104 19 0.9594857161010495 1104 20 0.8189836627983524 1104 21 0.6890191372365706 1104 22 0.7507870590183217 1104 23 1.0026978035305092 1104 24 0.8557054974416971 1104 25 0.8386028108331871 1105 1 1.4819591394245393 1105 2 4.270594520618566 1105 3 2.5210251865071704 1105 4 1.5306219610948588 1105 5 1.1019322641703155 1105 6 0.7924836715991432 1105 7 0.7434144306561947 1105 8 0.6711249686685221 1105 9 0.6843340389157433 1105 10 0.5800651867162543 1105 11 0.7350344931434378 1105 12 0.6802914123061683 1105 13 0.6873270486073579 1105 14 0.7154121787767878 1105 15 0.6610437428149183 1105 16 0.652765073418017 1105 17 0.8401937647966378 1105 18 0.8304775203524812 1105 19 0.8622924581009812 1105 20 0.7738250173282069 1105 21 0.8581277438301242 1105 22 0.9631811017536265 1105 23 0.6388778265386748 1105 24 0.7646032594067549 1105 25 0.8643252764215745 1106 1 0.5187557097646867 1106 2 2.2809769057142546 1106 3 1.1421456162571886 1106 4 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0.6092642054182207 1107 16 0.6952427025179659 1107 17 0.6098294838055054 1107 18 0.7949362824683206 1107 19 0.7667646444182594 1107 20 0.8203229289344875 1107 21 0.8871745258430224 1107 22 0.9012905488692482 1107 23 0.9110957106319901 1107 24 0.9804006204214062 1107 25 0.857737449248198 1108 1 1.400944775530803 1108 2 3.57377549764081 1108 3 2.219920042208596 1108 4 1.4734200306093186 1108 5 1.0569371960558414 1108 6 0.8631020162684138 1108 7 0.6953239277205583 1108 8 0.6227531067690961 1108 9 0.6632165576100704 1108 10 0.595455489621223 1108 11 0.5772105907918501 1108 12 0.5338609272569244 1108 13 0.6354607278263771 1108 14 0.6417930499976876 1108 15 0.7549233101574444 1108 16 0.7261587584651821 1108 17 0.773385597477521 1108 18 0.7372203138515374 1108 19 0.6567840460190126 1108 20 0.6777490348489579 1108 21 0.7187642957954736 1108 22 0.8254374126384292 1108 23 0.8372521033278204 1108 24 0.8148676493132285 1108 25 0.9024280223618888 1109 1 0.8703439595998859 1109 2 2.9345386229192627 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2315 15 -1.4536816435437103 2315 16 -1.3298301727026214 2315 17 -1.3564427505061758 2315 18 -1.175688420299032 2315 19 -1.1690095229700574 2315 20 -1.2974820759718995 2315 21 -1.2414885921552212 2315 22 -1.4862246976094866 2315 23 -1.236176168638174 2315 24 -1.1676739671212175 2315 25 -1.1847137259916494 2316 1 -0.5984182227244688 2316 2 -0.053183932149764246 2316 3 -0.06113401245935535 2316 4 -0.3990744574306433 2316 5 -0.787525450430401 2316 6 -0.782843604413614 2316 7 -0.9748734386612341 2316 8 -1.1052615799110086 2316 9 -1.1097659847020438 2316 10 -1.2945823979273925 2316 11 -1.440729240653667 2316 12 -1.4105193833655036 2316 13 -1.4143823998270904 2316 14 -1.6244817108411596 2316 15 -1.367326686422878 2316 16 -1.4963349326951345 2316 17 -1.6159182196010775 2316 18 -1.6547839840278513 2316 19 -1.479676134281597 2316 20 -1.4402498911831714 2316 21 -1.4252043731557507 2316 22 -1.5285256078792813 2316 23 -1.5073623267695808 2316 24 -1.614547324318842 2316 25 -1.514556005283687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90221.0 3 3.0 4 773.0 5 3671.0 6 4081.0 7 3796.0 8 3544.0 9 3412.0 10 3465.0 11 3300.0 12 3246.0 13 3180.0 14 3305.0 15 3438.0 16 3592.0 17 3915.0 18 4443.0 19 5536.0 20 7245.0 21 9271.0 22 11369.0 23 13294.0 24 15136.0 25 17313.0 26 19904.0 27 22260.0 28 26122.0 29 31274.0 30 39707.0 31 50114.0 32 65065.0 33 86532.0 34 119186.0 35 173477.0 36 276636.0 37 387690.0 38 942488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.959499456319456 18.714597782043427 12.86621232635136 25.459690435285758 2 17.161258606219626 21.30777499707479 38.966066725086684 22.5648996716189 3 18.59196490538008 24.339131508928876 29.633230990278765 27.43567259541228 4 12.249512800466801 16.868278149893296 38.327975086590676 32.55423396304923 5 13.353574394840479 37.466091075024664 35.84398887608472 13.336345654050136 6 31.791413331404577 37.273613460539025 18.124779307281873 12.810193900774522 7 28.070169735603844 31.486092667870512 22.307927983863497 18.13580961266215 8 25.046972775294595 37.10170662332386 20.486997155627794 17.36432344575376 9 26.234455531157202 15.664907493039426 20.24604592272097 37.8545910530824 10 14.679398618447786 29.176960585127997 34.51629418935392 21.627346607070297 11 34.29387704682889 22.67519490028858 24.44374283320371 18.587185219678815 12 22.96851203817629 24.631776299429013 31.936762394534913 20.462949267859784 13 28.771997119874655 21.86554755701332 25.83616280184835 23.526292521263677 14 21.005720842860285 21.79117359155888 27.76581561730369 29.437289948277147 15 22.99870296838201 30.44761406320347 23.47777573705691 23.075907231357608 16 22.405408524325846 28.20340803997068 27.275900404875408 22.115283030828067 17 21.912845326541525 28.304626892113948 27.679759967883026 22.102767813461497 18 21.714617520089462 27.69452318414688 29.195056302499157 21.395802993264503 19 23.37608721711022 27.076841183509853 27.505124980444823 22.0419466189351 20 23.41187637800459 27.345056006007347 27.983296126024282 21.25977148996378 21 23.22233071765871 26.817024274939627 27.096267960159825 22.864377047241838 22 23.094030187345208 27.64521234556363 27.47934245306211 21.781415014029047 23 22.677759830961307 27.354524121537192 27.537662918152357 22.43005312934914 24 22.932357254338985 26.88070676874429 28.157950234111862 22.028985742804867 25 22.93585101930524 27.202507933798998 27.790563960032344 22.071077086863415 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 203.0 2 381.5 3 560.0 4 560.0 5 560.0 6 1429.0 7 2298.0 8 2298.0 9 2298.0 10 3521.0 11 4744.0 12 4744.0 13 4744.0 14 8299.0 15 11854.0 16 11854.0 17 11854.0 18 20603.0 19 29352.0 20 29352.0 21 29352.0 22 45684.5 23 62017.0 24 62017.0 25 62017.0 26 87445.5 27 112874.0 28 112874.0 29 112874.0 30 145360.0 31 177846.0 32 177846.0 33 177846.0 34 203187.5 35 228529.0 36 228529.0 37 228529.0 38 249683.5 39 270838.0 40 270838.0 41 270838.0 42 287443.0 43 304048.0 44 304048.0 45 304048.0 46 311388.5 47 318729.0 48 318729.0 49 318729.0 50 310854.0 51 302979.0 52 302979.0 53 302979.0 54 273463.5 55 243948.0 56 243948.0 57 243948.0 58 210261.0 59 176574.0 60 176574.0 61 176574.0 62 143789.5 63 111005.0 64 111005.0 65 111005.0 66 85611.0 67 60217.0 68 60217.0 69 60217.0 70 43517.5 71 26818.0 72 26818.0 73 26818.0 74 18635.0 75 10452.0 76 10452.0 77 10452.0 78 6990.0 79 3528.0 80 3528.0 81 3528.0 82 2297.0 83 1066.0 84 1066.0 85 1066.0 86 710.5 87 355.0 88 355.0 89 355.0 90 234.0 91 113.0 92 113.0 93 113.0 94 78.0 95 43.0 96 43.0 97 43.0 98 28.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.458182107790154 3 0.0 4 0.0 5 0.0 6 1.2190146785620828E-4 7 0.0 8 1.6253529047494437E-4 9 0.0 10 1.6253529047494437E-4 11 8.126764523747218E-5 12 0.0 13 0.0 14 1.2190146785620828E-4 15 0.0 16 0.0 17 0.0 18 4.876058714248331E-4 19 7.720426297559858E-4 20 7.314088071372496E-4 21 0.0028850014059302624 22 0.0015847190821307075 23 0.0011783808559433467 24 4.063382261873609E-5 25 5.688735166623053E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2461004.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.8957638310847 #Duplication Level Percentage of deduplicated Percentage of total 1 81.66019284121529 58.71021938912846 2 12.318933830908275 17.713583147154814 3 3.289839264542163 7.095765204172581 4 1.2161643825356545 3.4974826890624144 5 0.5432061015495218 1.952710879430431 6 0.28103627620195265 1.2123190645069843 7 0.16398030448626774 0.8252642471005844 8 0.10711518533491846 0.616090245404973 9 0.070557655790741 0.4565516901485471 >10 0.3217037367598718 3.926628305613041 >50 0.015372688085815378 0.769483784113308 >100 0.009728200131842217 1.398563666890809 >500 0.0013702310259504121 0.7008436365264217 >1k 7.993014317935576E-4 1.1244940507466228 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3996 0.16237275518446942 No Hit GTCCTACAGTGGACATTTCTAAATT 2914 0.11840695911099698 No Hit CTGTAGGACGTGGAATATGGCAAGA 2706 0.10995512400629986 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2552 0.10369751532301451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.063382261873609E-5 0.0 9 0.0 0.0 0.0 8.126764523747218E-5 0.0 10 0.0 0.0 0.0 8.126764523747218E-5 0.0 11 0.0 0.0 0.0 8.126764523747218E-5 0.0 12 0.0 0.0 0.0 8.126764523747218E-5 0.0 13 0.0 0.0 0.0 8.126764523747218E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1010 0.0 13.007762 1 ACCGGTG 75 1.5360718E-5 12.620439 7 GGCGAGG 725 0.0 11.750542 19 TCGGTGA 75 2.1464017E-4 11.358395 13 TGCGCCC 60 0.0060394206 11.042885 12 GTATAAC 250 0.0 10.980882 1 GCGCCAC 105 3.6235542E-6 10.81752 13 GTATCAA 2085 0.0 10.815359 1 TAGGACC 650 0.0 10.775913 4 GTACCGT 80 3.9019575E-4 10.648496 6 AACAGCG 90 9.878732E-5 10.517032 7 CGGTAGG 95 1.19590375E-4 10.320377 1 AGGACGT 1840 0.0 10.2369585 5 GTCCTAC 1700 0.0 10.208067 1 GGACGTG 1785 0.0 10.128168 6 GCACTCG 85 6.821275E-4 10.022114 13 TGTAGGA 1850 0.0 9.963347 2 GAACAGT 545 0.0 9.899519 6 GACGTGG 910 0.0 9.881389 7 GTAGGAC 1880 0.0 9.868109 3 >>END_MODULE