FastQCFastQC Report
Thu 26 May 2016
SRR1512026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461004
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48450.1968708705877764No Hit
GTCCTACAGTGGACATTTCTAAATT32820.13336020583469185No Hit
CTGTAGGACGTGGAATATGGCAAGA31670.1286873162335372No Hit
TATCAACGCAGAGTACTTTTTTTTT31440.12775273831330627No Hit
GGTATCAACGCAGAGTACTTTTTTT29980.12182020021097081No Hit
GTCCTAAAGTGTGTATTTCTCATTT28470.11568449299554165No Hit
CTTTAGGACGTGAAATATGGCGAGG27710.11259632247651771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.012.8100311
TAGGACC5850.012.1856014
GGCGAGG8150.010.83755819
GGACGTG18100.010.6027896
TCCAACG2150.010.59984318
AGGACGT18500.010.5836895
GGCGAAA909.545485E-510.55269919
TGTAGGA20200.010.4928882
GTCCTAC17400.010.4781481
GTAGGAC19600.010.4746453
TGGCGAG17750.010.32489418
CGTGCGC1201.5354799E-610.28595510
GTATCAA24350.010.2315641
AAGTCGT1502.467641E-810.1277097
GACGTGG9600.010.0881487
GAACCGC856.5925485E-410.0593726
GTCCTAA10250.09.9646121
TAGGACG19900.09.8391084
CAAGTCG1651.02190825E-89.7884136
GGTCTAC2550.09.7327071
Produced by FastQC (version 0.11.5)