FastQCFastQC Report
Thu 26 May 2016
SRR1512025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135340
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31990.14981220789195163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1450.013.5269091
TCCAACG1500.013.25141518
CCAACGA1251.4952093E-912.11557919
CTAGTAC752.146683E-411.3580893
GGTATCA9400.011.1632981
CCGTTTC1451.2951205E-911.09698413
GATCGTG700.001538335310.8172287
ATAGGAC2650.010.7151793
GGTCTAA1201.0129224E-610.624261
GTCAACG650.00840959110.5613942
GTGGTAT4050.010.412381
CGAAATC1106.319113E-610.32553713
TAGGACC4950.010.3255374
ATGTCGA750.00272987810.09631617
TAATACG750.002730408210.096084
GTATCAA18450.09.8867411
ACGAAAT1356.777518E-79.81563312
TAGGACG12450.09.8071854
GTCTAGG2800.09.8070091
ACTGTTC6350.09.688668
Produced by FastQC (version 0.11.5)