FastQCFastQC Report
Thu 26 May 2016
SRR1512025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135340
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41370.1937396386523926No Hit
GTCCTACAGTGGACATTTCTAAATT26410.12368053799394944No Hit
TATCAACGCAGAGTACTTTTTTTTT25120.11763934549064786No Hit
GGTATCAACGCAGAGTACTTTTTTT24890.11656223364897393No Hit
CTGTAGGACGTGGAATATGGCAAGA23040.10789850796594452No Hit
GTCCTAAAGTGTGTATTTCTCATTT21390.10017140127567506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4300.013.7055854
GGTATCA8300.012.4218751
TCCAACG1155.3605618E-912.38626418
AATCCCG2250.011.81879719
ACGGTAC657.9868024E-411.69905953
GGACCGC752.0733196E-411.4007026
GTAGGAC14300.011.034343
CTGTAGG15250.010.7671341
AGGACGT13950.010.697935
TACACCG803.7518478E-410.6936725
GGCGAGG6400.010.68440419
GTATCAA20250.010.6071571
TGTAGGA15600.010.6022732
ACCGTCC909.575484E-510.5492858
GGACGTG13900.010.5258296
TGGCGAG13150.010.47102418
AGGACCT9450.010.4610635
ACTGTTC6200.010.2600318
TAGGACG15150.010.101544
GACGTGG8100.010.0804287
Produced by FastQC (version 0.11.5)