FastQCFastQC Report
Thu 26 May 2016
SRR1512024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992419
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA37290.18715942781111805No Hit
GTCCTACAGTGGACATTTCTAAATT35900.18018298359933327No Hit
GTATCAACGCAGAGTACTTTTTTTT35680.1790787981845184No Hit
GTCCTAAAGTGTGTATTTCTCATTT35620.17877765670775073No Hit
CTTTAGGACGTGAAATATGGCGAGG34330.17230311495724543No Hit
GGTATCAACGCAGAGTACTTTTTTT22470.11277748304949914No Hit
TATCAACGCAGAGTACTTTTTTTTT22380.1123257708343476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG350.00222431816.2260885
CGTAAAG450.00933084813.0750691
CGGTAGG1051.27965905E-813.0750691
CGTTAGG550.002419152312.4807481
GGTATCA10700.012.1891421
CGTAGGA656.147589E-412.0692962
TAAAACG550.003154429112.0466414
TAGGACC7050.011.4119654
TCCAACG1501.8735591E-1011.35826118
AGGACGT23100.010.8173915
AGTACCG700.001538063610.8173915
GACGTGG12050.010.7612857
CCGTAGG650.008413739510.5606341
CGGTGTG909.8777426E-510.5169099
GGACGTG23250.010.5033386
CGATATT854.9239764E-410.3831431
GGCGAGG9950.010.36893219
CCAACGA1201.5974492E-610.25398519
GTAGGAC23450.010.2523043
GGCGTGT750.00272992910.0962328
Produced by FastQC (version 0.11.5)