##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512024_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1992419 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.501021120557475 31.0 30.0 34.0 25.0 34.0 2 26.544425143506462 31.0 25.0 34.0 10.0 34.0 3 28.51776157525099 31.0 28.0 34.0 16.0 34.0 4 33.036707640310595 35.0 33.0 37.0 27.0 37.0 5 33.500162867348685 35.0 35.0 37.0 28.0 37.0 6 33.78706737889972 37.0 35.0 37.0 29.0 37.0 7 33.86202099056474 37.0 35.0 37.0 30.0 37.0 8 33.89668789546777 37.0 35.0 37.0 30.0 37.0 9 35.525848729609585 39.0 37.0 39.0 30.0 39.0 10 35.43790989746635 39.0 37.0 39.0 30.0 39.0 11 35.441121571316074 39.0 37.0 39.0 30.0 39.0 12 35.34955649389009 39.0 37.0 39.0 30.0 39.0 13 35.34877904697757 39.0 37.0 39.0 30.0 39.0 14 36.55434675136103 40.0 37.0 41.0 30.0 41.0 15 36.495011340486116 40.0 37.0 41.0 29.0 41.0 16 36.44248925552306 40.0 37.0 41.0 29.0 41.0 17 36.41719387337704 40.0 37.0 41.0 29.0 41.0 18 36.37145249066587 40.0 37.0 41.0 29.0 41.0 19 36.40344676496259 40.0 37.0 41.0 29.0 41.0 20 36.30829860586553 40.0 37.0 41.0 29.0 41.0 21 36.24820482037162 40.0 37.0 41.0 29.0 41.0 22 36.15337436553255 40.0 37.0 41.0 28.0 41.0 23 36.05940216390227 40.0 37.0 41.0 28.0 41.0 24 35.96844187894213 40.0 37.0 41.0 27.0 41.0 25 35.87410880944219 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1517114367471635 1101 2 4.102620878514411 1101 3 2.2364243199651277 1101 4 1.3519610689128214 1101 5 0.8980249217604026 1101 6 0.5488572428141367 1101 7 0.19221151670830494 1101 8 0.23971515995051362 1101 9 0.20941742792900442 1101 10 0.45086603778088374 1101 11 0.49282325301195584 1101 12 0.37947615276832636 1101 13 0.4901338946555782 1101 14 0.29249042707473905 1101 15 0.35027204236405396 1101 16 0.2873919073780371 1101 17 0.48601731006341 1101 18 0.4043547237914993 1101 19 0.2650005538775275 1101 20 0.27096822739446935 1101 21 0.4393948320803389 1101 22 0.4688861854898647 1101 23 0.42906648572403583 1101 24 0.3902331348465182 1101 25 0.3613406175484357 1102 1 1.8300049178055886 1102 2 4.469413907202455 1102 3 2.694312078687517 1102 4 1.920939302653963 1102 5 1.4513447609748624 1102 6 1.2469934265679612 1102 7 0.9720826532154234 1102 8 1.0790914639132367 1102 9 1.1663597498413552 1102 10 0.9604990599701253 1102 11 1.0369778389225743 1102 12 1.0199006235704076 1102 13 1.04394428625843 1102 14 1.183005481942132 1102 15 1.0575816500572799 1102 16 1.1377771999826365 1102 17 1.0554219881293676 1102 18 1.1108199277258493 1102 19 1.1882349188164483 1102 20 1.2530349393767395 1102 21 1.2602102803638289 1102 22 1.2000524777214423 1102 23 1.3103251532756417 1102 24 1.2665011153513817 1102 25 1.274998683364167 1103 1 1.3693602756245973 1103 2 4.086079178224502 1103 3 2.405991925093794 1103 4 1.4899527154055434 1103 5 1.1653838535874144 1103 6 0.982358879145167 1103 7 0.9307497276530441 1103 8 0.8605131888814412 1103 9 0.8572205227614802 1103 10 0.7532846695391484 1103 11 0.7775784758219615 1103 12 0.6095553168198578 1103 13 0.9266009139926794 1103 14 0.7767715621953357 1103 15 1.0033735994936777 1103 16 0.8234892578666972 1103 17 1.0354976442037014 1103 18 0.9367096208296672 1103 19 0.934109575366513 1103 20 0.9679845384019714 1103 21 1.204739171421025 1103 22 1.1465738031712007 1103 23 1.2775405509979478 1103 24 1.3385934414904739 1103 25 1.4898654539153355 1104 1 1.5065492455374816 1104 2 4.14275780134232 1104 3 2.3584717000202673 1104 4 1.6668241145538687 1104 5 1.317590868348006 1104 6 1.1098345371558764 1104 7 1.0059002560098946 1104 8 0.8937961334851536 1104 9 1.0153324124142245 1104 10 0.9756619042706163 1104 11 1.012025811771565 1104 12 1.109847849778376 1104 13 1.0456899507039665 1104 14 1.0206408367831799 1104 15 1.0286352265915681 1104 16 1.125802790574724 1104 17 1.1611012652340662 1104 18 1.2386239286775549 1104 19 1.3077773762144815 1104 20 1.1758183332937477 1104 21 1.0791961444326077 1104 22 1.1300991819274557 1104 23 1.2883596316848624 1104 24 1.2182757083686653 1104 25 1.2415904996072982 1105 1 1.2859346298880787 1105 2 4.049418770789078 1105 3 2.307147856503427 1105 4 1.3350637604720106 1105 5 1.0740778807497762 1105 6 0.8691467227274288 1105 7 0.6591171039994492 1105 8 0.5797607752498237 1105 9 0.5823018027067235 1105 10 0.32856205705311226 1105 11 0.4241210385944001 1105 12 0.49733608161685794 1105 13 0.5343767704716527 1105 14 0.6194149391181369 1105 15 0.4758554972565676 1105 16 0.6288440527080112 1105 17 0.7451120935714712 1105 18 0.5664490747004862 1105 19 0.6191452845058905 1105 20 0.6264072571598547 1105 21 0.7466433736617404 1105 22 0.8373317555470123 1105 23 0.4537097663875542 1105 24 0.6824931677053954 1105 25 0.8166491096666206 1106 1 0.14969425214155052 1106 2 1.9454930942548287 1106 3 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2315 13 -1.3181402210635582 2315 14 -1.5290922510899136 2315 15 -1.7934088337509309 2315 16 -1.545038224174455 2315 17 -1.5051190382037873 2315 18 -1.5157584682347576 2315 19 -1.5329143005958272 2315 20 -1.6286005979707667 2315 21 -1.599991481679922 2315 22 -1.8677574981653322 2315 23 -1.8213663563601656 2315 24 -1.6775498429032183 2315 25 -1.5504149473324844 2316 1 -0.7069021986106243 2316 2 -0.39807611962656253 2316 3 -0.21734271773662783 2316 4 -0.4407668107265721 2316 5 -0.8793707602640026 2316 6 -1.0259421844385699 2316 7 -1.1690356508766513 2316 8 -1.3548703832225257 2316 9 -1.400312274188238 2316 10 -1.5770869895426358 2316 11 -1.6703758176278285 2316 12 -1.5788657953343161 2316 13 -1.6954323098190827 2316 14 -1.9443433980435714 2316 15 -1.6356618283547348 2316 16 -1.6339914142964531 2316 17 -1.887671015619567 2316 18 -1.8546385999549386 2316 19 -1.645170907004598 2316 20 -1.616851953030178 2316 21 -1.7759510610912415 2316 22 -1.8901516378061913 2316 23 -1.7180469115028174 2316 24 -1.8552236064609318 2316 25 -1.7759577775833435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65219.0 3 0.0 4 626.0 5 2752.0 6 3052.0 7 2801.0 8 2573.0 9 2541.0 10 2504.0 11 2360.0 12 2474.0 13 2434.0 14 2371.0 15 2456.0 16 2718.0 17 2864.0 18 3266.0 19 4167.0 20 5390.0 21 6757.0 22 8274.0 23 10126.0 24 11766.0 25 13390.0 26 15269.0 27 17413.0 28 20037.0 29 24506.0 30 30421.0 31 39254.0 32 50906.0 33 68231.0 34 95170.0 35 139135.0 36 226645.0 37 313395.0 38 789156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86614361738169 19.480290039394323 13.32134455654157 25.332221786682418 2 16.641646417337757 21.86074736273399 39.61855516249989 21.879051057428363 3 18.113710017822555 25.19409822933831 30.17292045498462 26.51927129785452 4 11.964049730503474 17.64653920686362 39.400497586100116 30.988913476532797 5 12.843985125618657 37.98558435750713 36.31731076646027 12.853119750413944 6 30.389491367026718 38.132240256692995 18.910630745842113 12.567637630438174 7 27.20798185522222 31.865385744665154 23.20937513645473 17.717257263657896 8 24.198536652449437 37.69384737540014 21.271440029150508 16.836175942999912 9 26.01485932426864 15.809024105873313 21.111673799537144 37.0644427703209 10 14.663338717076439 29.292246908028336 35.14237474264939 20.902039632245845 11 32.99516818500526 22.797865308451687 25.870913698373688 18.336052808169363 12 23.09885621448099 24.956698365153112 32.40739021260086 19.537055207765032 13 28.371692901944822 22.616628329683667 26.286589316805355 22.72508945156616 14 20.324991881727687 22.923541684756067 28.353373462108124 28.398092971408122 15 22.14443849411193 31.961249114769537 23.772108176041286 22.12220421507725 16 21.337831048589678 28.690300584365037 28.70395233131184 21.267916035733446 17 21.025045434720308 29.22828983261051 28.50258906384651 21.244075668822674 18 20.807031473896227 27.885182439584565 30.891486855385907 20.4162992311333 19 23.101111121149245 27.294792918296608 28.091469492602393 21.512626467951748 20 23.111944497283936 27.85239160472735 28.776951697118108 20.258712200870605 21 22.42229284785018 27.280621095067538 27.655052922375162 22.64203313470712 22 22.709025970897176 28.741096093925666 27.900595467540395 20.649282467636763 23 21.60929774537906 28.44237368355805 28.497784327119547 21.45054424394335 24 22.541443340984 27.360911535174075 29.163092702890303 20.934552420951615 25 22.144348876159466 27.924738344386117 28.743797081824063 21.187115697630357 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 277.0 1 277.0 2 395.0 3 513.0 4 513.0 5 513.0 6 1249.5 7 1986.0 8 1986.0 9 1986.0 10 3026.5 11 4067.0 12 4067.0 13 4067.0 14 7129.0 15 10191.0 16 10191.0 17 10191.0 18 18254.0 19 26317.0 20 26317.0 21 26317.0 22 41937.0 23 57557.0 24 57557.0 25 57557.0 26 81877.5 27 106198.0 28 106198.0 29 106198.0 30 136746.0 31 167294.0 32 167294.0 33 167294.0 34 188836.5 35 210379.0 36 210379.0 37 210379.0 38 223909.5 39 237440.0 40 237440.0 41 237440.0 42 247327.5 43 257215.0 44 257215.0 45 257215.0 46 257587.0 47 257959.0 48 257959.0 49 257959.0 50 244785.5 51 231612.0 52 231612.0 53 231612.0 54 202210.0 55 172808.0 56 172808.0 57 172808.0 58 145733.5 59 118659.0 60 118659.0 61 118659.0 62 94704.5 63 70750.0 64 70750.0 65 70750.0 66 53758.5 67 36767.0 68 36767.0 69 36767.0 70 26306.0 71 15845.0 72 15845.0 73 15845.0 74 10817.0 75 5789.0 76 5789.0 77 5789.0 78 3863.5 79 1938.0 80 1938.0 81 1938.0 82 1267.5 83 597.0 84 597.0 85 597.0 86 387.5 87 178.0 88 178.0 89 178.0 90 118.0 91 58.0 92 58.0 93 58.0 94 37.0 95 16.0 96 16.0 97 16.0 98 12.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.478685959128075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.0190246127947984E-5 9 0.0 10 5.0190246127947984E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.0114147676768793E-4 19 8.532341841751158E-4 20 6.022829535353759E-4 21 0.003362746490572515 22 0.0017566586144781794 23 0.0011041854148148558 24 0.0 25 5.019024612794798E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1992419.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.36951513802424 #Duplication Level Percentage of deduplicated Percentage of total 1 86.09916548052593 65.75351521536278 2 10.11387483345209 15.447834343948038 3 2.1474593885954163 4.920012968568898 4 0.7315857326841182 2.2348339074792927 5 0.3009399632518752 1.149131953960027 6 0.15733322661790472 0.7209277339146164 7 0.10167892838096149 0.5435619322145545 8 0.06247619658670959 0.3817021472795917 9 0.044499180855960864 0.3058542779408084 >10 0.20943865739042378 2.820508672622147 >50 0.015047111856521158 0.8208306889134974 >100 0.013309140495699074 1.9921852882974858 >500 0.0020616028853702 1.0854467393445577 >1k 0.0011305564210094476 1.823654130153687 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3729 0.18715942781111805 No Hit GTCCTACAGTGGACATTTCTAAATT 3590 0.18018298359933327 No Hit GTATCAACGCAGAGTACTTTTTTTT 3568 0.1790787981845184 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3562 0.17877765670775073 No Hit CTTTAGGACGTGAAATATGGCGAGG 3433 0.17230311495724543 No Hit GGTATCAACGCAGAGTACTTTTTTT 2247 0.11277748304949914 No Hit TATCAACGCAGAGTACTTTTTTTTT 2238 0.1123257708343476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0038049225589597E-4 0.0 0.0 0.0 6 0.0 1.0038049225589597E-4 0.0 0.0 0.0 7 0.0 1.0038049225589597E-4 0.0 0.0 0.0 8 5.0190246127947984E-5 1.0038049225589597E-4 0.0 0.0 0.0 9 5.0190246127947984E-5 1.5057073838384397E-4 0.0 0.0 0.0 10 5.0190246127947984E-5 1.5057073838384397E-4 0.0 5.0190246127947984E-5 0.0 11 5.0190246127947984E-5 1.5057073838384397E-4 0.0 5.0190246127947984E-5 0.0 12 5.0190246127947984E-5 1.5057073838384397E-4 0.0 5.0190246127947984E-5 0.0 13 5.0190246127947984E-5 1.5057073838384397E-4 0.0 5.0190246127947984E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 35 0.002224318 16.226088 5 CGTAAAG 45 0.009330848 13.075069 1 CGGTAGG 105 1.27965905E-8 13.075069 1 CGTTAGG 55 0.0024191523 12.480748 1 GGTATCA 1070 0.0 12.189142 1 CGTAGGA 65 6.147589E-4 12.069296 2 TAAAACG 55 0.0031544291 12.046641 4 TAGGACC 705 0.0 11.411965 4 TCCAACG 150 1.8735591E-10 11.358261 18 AGGACGT 2310 0.0 10.817391 5 AGTACCG 70 0.0015380636 10.817391 5 GACGTGG 1205 0.0 10.761285 7 CCGTAGG 65 0.0084137395 10.560634 1 CGGTGTG 90 9.8777426E-5 10.516909 9 GGACGTG 2325 0.0 10.503338 6 CGATATT 85 4.9239764E-4 10.383143 1 GGCGAGG 995 0.0 10.368932 19 CCAACGA 120 1.5974492E-6 10.253985 19 GTAGGAC 2345 0.0 10.252304 3 GGCGTGT 75 0.002729929 10.096232 8 >>END_MODULE