FastQCFastQC Report
Thu 26 May 2016
SRR1512024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992419
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44190.22179069763940215No Hit
GTCCTACAGTGGACATTTCTAAATT42980.21571767785792043No Hit
CTGTAGGACGTGGAATATGGCAAGA40370.20261802361852604No Hit
GTCCTAAAGTGTGTATTTCTCATTT40340.20246745288014217No Hit
CTTTAGGACGTGAAATATGGCGAGG39860.20005832106600066No Hit
TATCAACGCAGAGTACTTTTTTTTT27210.13656765971414647No Hit
GGTATCAACGCAGAGTACTTTTTTT26970.1353630938070757No Hit
GTCCTACAGTGTGCATTTCTCATTT21040.10560027785320256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATT350.002163925216.2930984
TCCAACG1052.73978E-711.75572118
TAGGACC7250.011.6673574
TATGTCG1501.7826096E-1011.39372116
GGCGAGG10500.011.03312319
CTAAGAC2650.010.7595943
GGTATCA10400.010.7433551
ATGTCGA1803.6379788E-1110.54974217
GTGGTAT4800.010.5444031
ATTTAGA6800.010.3922631
TTAGGAC14600.010.2854843
AGGACGT23500.010.1918545
GTATCAA21600.010.1686081
GTCCTAG2450.010.1343191
GGACGTG23150.010.09536
CCTACAC3300.010.0803273
TGTCGAG1701.5734258E-910.05353518
AATCCCG2002.5465852E-119.97050719
TGGTATC4300.09.9463692
AATGTCC6800.09.9136548
Produced by FastQC (version 0.11.5)