FastQCFastQC Report
Thu 26 May 2016
SRR1512023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237098
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32240.1441152779180885No Hit
GTCCTACAGTGGACATTTCTAAATT28230.1261902697154975No Hit
CTGTAGGACGTGGAATATGGCAAGA25660.11470217218914863No Hit
GTCCTAAAGTGTGTATTTCTCATTT24140.10790765536422633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG506.632138E-515.6944981
CGCCCAC705.2182986E-614.0129451
TAGGACC7000.012.1690984
GGTATCA10250.011.5794781
AGGACGG951.4178791E-510.9593045
GGCGAGG6700.010.45395219
GCGCCAC1304.043668E-710.19291913
GTAGGAC17550.010.1929193
GTCTATA1958.913048E-1110.0605761
GGACGTG16000.010.0564066
CGTGCGC856.8239134E-410.02160910
AGGACGT16550.010.0081535
TGTAGGA17500.09.9772172
GTATCAA19200.09.911241
TAGGACG17350.09.8194444
AGGACCT13450.09.640785
TGGCGAG15550.09.55636718
TAGACTG3800.09.4648535
TGGACCG900.00115231639.4648535
TCTAGAC3200.09.4648533
Produced by FastQC (version 0.11.5)