##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512023_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2237098 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.22791759681516 31.0 30.0 34.0 23.0 34.0 2 26.280603710700202 31.0 25.0 34.0 10.0 34.0 3 28.251939789852745 31.0 27.0 34.0 16.0 34.0 4 32.7350969872576 35.0 33.0 37.0 26.0 37.0 5 33.186241729240294 35.0 35.0 37.0 27.0 37.0 6 33.46767955628229 37.0 35.0 37.0 28.0 37.0 7 33.52642664737977 37.0 35.0 37.0 28.0 37.0 8 33.56224850230075 37.0 35.0 37.0 28.0 37.0 9 35.159741772600036 39.0 35.0 39.0 28.0 39.0 10 35.06827416590601 39.0 35.0 39.0 28.0 39.0 11 35.07428999534218 39.0 35.0 39.0 28.0 39.0 12 34.96370342291665 39.0 35.0 39.0 27.0 39.0 13 34.96519776961045 39.0 35.0 39.0 27.0 39.0 14 36.154480045129894 40.0 37.0 41.0 27.0 41.0 15 36.093293633090724 40.0 36.0 41.0 27.0 41.0 16 36.03373254099731 40.0 36.0 41.0 27.0 41.0 17 35.99800545170574 40.0 36.0 41.0 27.0 41.0 18 35.94254878418379 40.0 36.0 41.0 27.0 41.0 19 35.962643567693505 40.0 37.0 41.0 27.0 41.0 20 35.858814410454976 40.0 36.0 41.0 27.0 41.0 21 35.80111555238081 40.0 36.0 41.0 27.0 41.0 22 35.69684788060246 40.0 36.0 41.0 27.0 41.0 23 35.59866398342853 40.0 36.0 41.0 27.0 41.0 24 35.50463725773301 40.0 36.0 41.0 26.0 41.0 25 35.4037865127053 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2504170201630203 1101 2 4.151130611794425 1101 3 2.2931418851505363 1101 4 1.4354011662412205 1101 5 0.993545011319199 1101 6 0.7523268935619427 1101 7 0.4647762889406053 1101 8 0.3871798450303885 1101 9 0.3748149603179982 1101 10 0.6271763562510486 1101 11 0.537802176925652 1101 12 0.515275194221914 1101 13 0.5154506904297023 1101 14 0.4126805070980666 1101 15 0.5985373434607126 1101 16 0.6603283674118785 1101 17 0.556076432091686 1101 18 0.7038267549174861 1101 19 0.5467358166347722 1101 20 0.5025277163105386 1101 21 0.6274438052622173 1101 22 0.6733757838208732 1101 23 0.5700465582070109 1101 24 0.6126068525455679 1101 25 0.5620687404812443 1102 1 1.7203756636700476 1102 2 4.422677344631481 1102 3 2.6917026791952026 1102 4 1.8276261455629736 1102 5 1.4001455075971094 1102 6 1.1557346685826175 1102 7 0.9549003584195148 1102 8 0.8629118549559465 1102 9 1.0944510231798645 1102 10 1.0072590692369872 1102 11 1.1292331115823941 1102 12 1.0142495531962794 1102 13 1.0724068525471537 1102 14 1.150612682449598 1102 15 1.0965687743953652 1102 16 1.1938602119114705 1102 17 1.2084006669965675 1102 18 1.2291534712119443 1102 19 1.1505406139879568 1102 20 1.2316923151525572 1102 21 1.1596522419867838 1102 22 1.0222095307191381 1102 23 1.231369965982033 1102 24 1.2604149584181883 1102 25 1.1466675824994468 1103 1 1.7207313417997767 1103 2 4.363661567713198 1103 3 2.6869156497712545 1103 4 1.8163437501555677 1103 5 1.5701419557318914 1103 6 1.4851786480481692 1103 7 1.1395307370680143 1103 8 1.129881106126895 1103 9 1.2195636935065508 1103 10 1.151059466701767 1103 11 1.2757348930455876 1103 12 1.2409421275857326 1103 13 1.2766912131561057 1103 14 1.3548407539834244 1103 15 1.5105257611846383 1103 16 1.1887438053666983 1103 17 1.4053841624989758 1103 18 1.3989675329111648 1103 19 1.3576246801239336 1103 20 1.5354030925440156 1103 21 1.4428329925979142 1103 22 1.4995204860725622 1103 23 1.4696853740716875 1103 24 1.4032495926682529 1103 25 1.5663259889286891 1104 1 1.308811563070627 1104 2 3.965936629213271 1104 3 2.30027143018982 1104 4 1.4150223368362589 1104 5 1.2203420147907806 1104 6 0.8335571755523219 1104 7 0.6151362377803693 1104 8 0.8017458965560564 1104 9 0.7479217268689453 1104 10 0.7664004259264203 1104 11 0.6545108680761516 1104 12 0.7634547423064504 1104 13 0.6237739744299446 1104 14 0.7888885587455476 1104 15 0.7286500178014137 1104 16 0.9117103029743419 1104 17 0.748052800932669 1104 18 0.7332999262433901 1104 19 0.9186220460031862 1104 20 0.8268568471954865 1104 21 0.7055874193678306 1104 22 0.7545341978533955 1104 23 1.0064641232231537 1104 24 0.8217547909096581 1104 25 0.8825498903685727 1105 1 1.7158940473659356 1105 2 4.412151993120375 1105 3 2.752953038464227 1105 4 1.891951046141351 1105 5 1.527726261541055 1105 6 1.2510743052027564 1105 7 1.2268024860937103 1105 8 0.9761628417291206 1105 9 1.031181199831444 1105 10 0.8986545903688778 1105 11 1.020285108545636 1105 12 1.0208520877175928 1105 13 0.987545161102851 1105 14 0.9719260669527685 1105 15 1.0788520879378893 1105 16 1.049171267320773 1105 17 1.2623774745348655 1105 18 1.1449238642379456 1105 19 1.2349447312396222 1105 20 1.1423052653266694 1105 21 1.3060267451649565 1105 22 1.1432298891064505 1105 23 1.0744870584850759 1105 24 1.2555966884288026 1105 25 1.3049658095712076 1106 1 0.4529275619955939 1106 2 2.3235386130159874 1106 3 1.1278316205182364 1106 4 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0.11365366202073801 1107 16 0.11212663536908707 1107 17 0.05712245009134875 1107 18 0.07127793508389857 1107 19 0.0635298393890551 1107 20 0.11403391430680188 1107 21 0.18961734915761497 1107 22 0.13884360518273553 1107 23 0.28255122793022736 1107 24 0.3773886491154457 1107 25 0.18315551242894657 1108 1 1.336522412561699 1108 2 3.5259774124214758 1108 3 2.1629014799806754 1108 4 1.378668615622388 1108 5 0.9161990054652804 1108 6 0.7324507685468689 1108 7 0.5933216896707023 1108 8 0.510147582722368 1108 9 0.5543915928916405 1108 10 0.613489595527092 1108 11 0.6956096993163285 1108 12 0.6440387464741377 1108 13 0.7327146976210273 1108 14 0.6877031444293848 1108 15 0.6549747865933213 1108 16 0.700601550122002 1108 17 0.700698174892139 1108 18 0.8132585743450349 1108 19 0.7787240649547869 1108 20 0.8373172932719868 1108 21 0.7069360274479024 1108 22 0.8421994099595906 1108 23 0.8401188003442428 1108 24 0.8033962541088044 1108 25 0.8407745209484645 1109 1 0.8778362344616362 1109 2 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-1.2152185430132576 2315 14 -1.3142775956264572 2315 15 -1.7338420046850587 2315 16 -1.474064830630951 2315 17 -1.4760152232116468 2315 18 -1.4676959316416927 2315 19 -1.4877498923034906 2315 20 -1.4701190043653796 2315 21 -1.577091902339184 2315 22 -1.717146929475561 2315 23 -1.676442573099635 2315 24 -1.5284054198535628 2315 25 -1.517543569350039 2316 1 -0.9012976442747274 2316 2 -0.20657984808923047 2316 3 -0.38959816702645966 2316 4 -0.8549798609195278 2316 5 -1.0827544745094286 2316 6 -1.0259711907486562 2316 7 -1.080360865640074 2316 8 -1.2672199928663233 2316 9 -1.3030023864226266 2316 10 -1.419944706200937 2316 11 -1.532899510805997 2316 12 -1.4660321012704287 2316 13 -1.652154655601727 2316 14 -1.6571022576073204 2316 15 -1.5929029273868736 2316 16 -1.798836969501231 2316 17 -1.836274150044865 2316 18 -1.8919041873923135 2316 19 -1.6947845771411352 2316 20 -1.6462888452422746 2316 21 -1.7971918422644393 2316 22 -1.7728170684371776 2316 23 -1.8310022163089954 2316 24 -1.8081102856559284 2316 25 -1.8669387990103203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87056.0 3 2.0 4 750.0 5 3499.0 6 4089.0 7 3780.0 8 3422.0 9 3319.0 10 3327.0 11 3161.0 12 3128.0 13 3029.0 14 3148.0 15 3141.0 16 3366.0 17 3624.0 18 4231.0 19 5186.0 20 6882.0 21 8577.0 22 10423.0 23 12337.0 24 14480.0 25 16449.0 26 18252.0 27 20621.0 28 23948.0 29 28649.0 30 36035.0 31 45465.0 32 58355.0 33 76966.0 34 106412.0 35 153716.0 36 250107.0 37 344772.0 38 863394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91716321770436 18.791040893157117 12.877084508591041 25.414711380547477 2 17.205230100130546 21.450192765435826 38.83151068618195 22.513066448251674 3 18.473978341583607 24.453331950589558 29.677287271277343 27.395402436549492 4 12.180512431730751 16.923934490129625 38.626202338923015 32.26935073921661 5 13.317878787607873 37.56406737657447 35.88322907624074 13.234824759576917 6 31.591343602892497 37.458053897528806 18.218834498459387 12.73176800111931 7 28.082185045089663 31.455126239440563 22.46834962080338 17.994339094666394 8 25.004872388131755 36.99769701434359 20.63389322586067 17.363537371663977 9 26.266216321323427 15.661316580677287 20.350874212931217 37.72159288506807 10 14.676623031207884 29.168586939758928 34.704918655667285 21.449871373365905 11 34.19452344063604 22.612151993341374 24.642192697861248 18.55113186816134 12 23.160004613119316 24.707411119226784 31.776256560955307 20.356327706698586 13 28.774599950471547 21.997963432983266 25.804189177228714 23.423247439316473 14 20.88871386054612 21.99720351991732 27.850590363050703 29.263492256485858 15 22.909635608274648 30.659720763238802 23.478542290056133 22.952101338430413 16 22.367459986107 28.26201623710718 27.396385853458366 21.974137923327454 17 21.77356557468649 28.602010282964805 27.64456452064237 21.979859621706336 18 21.623502685856486 27.67475947537179 29.388325632104937 21.313412206666786 19 23.36045201780893 27.07744023459152 27.568973840899748 21.993133906699804 20 23.55851476363214 27.26963883804147 28.015341392928743 21.156505005397648 21 23.115143447084012 26.827749508611205 27.101684512524884 22.955422531779902 22 23.144436259083367 27.83712164559135 27.359484965955254 21.65895712937003 23 22.49851033290316 27.587919414485956 27.616751680876426 22.296818571734455 24 22.990296348480353 26.857810304072782 28.309066754399453 21.842826593047416 25 22.820817589286793 27.250636876762613 27.886418466140594 22.042127067809997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 183.0 1 183.0 2 339.5 3 496.0 4 496.0 5 496.0 6 1174.0 7 1852.0 8 1852.0 9 1852.0 10 2963.0 11 4074.0 12 4074.0 13 4074.0 14 7228.0 15 10382.0 16 10382.0 17 10382.0 18 18526.0 19 26670.0 20 26670.0 21 26670.0 22 41465.5 23 56261.0 24 56261.0 25 56261.0 26 80243.5 27 104226.0 28 104226.0 29 104226.0 30 133210.5 31 162195.0 32 162195.0 33 162195.0 34 186777.0 35 211359.0 36 211359.0 37 211359.0 38 231454.0 39 251549.0 40 251549.0 41 251549.0 42 265860.0 43 280171.0 44 280171.0 45 280171.0 46 285512.5 47 290854.0 48 290854.0 49 290854.0 50 282755.5 51 274657.0 52 274657.0 53 274657.0 54 246468.0 55 218279.0 56 218279.0 57 218279.0 58 187066.0 59 155853.0 60 155853.0 61 155853.0 62 126846.0 63 97839.0 64 97839.0 65 97839.0 66 75226.0 67 52613.0 68 52613.0 69 52613.0 70 38138.0 71 23663.0 72 23663.0 73 23663.0 74 16449.5 75 9236.0 76 9236.0 77 9236.0 78 6214.0 79 3192.0 80 3192.0 81 3192.0 82 2101.5 83 1011.0 84 1011.0 85 1011.0 86 677.5 87 344.0 88 344.0 89 344.0 90 216.0 91 88.0 92 88.0 93 88.0 94 64.0 95 40.0 96 40.0 97 40.0 98 25.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.5097255462210417 3 0.0 4 0.0 5 0.0 6 4.470076858501505E-5 7 0.0 8 8.94015371700301E-5 9 0.0 10 1.3410230575504515E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.023069172651355E-4 19 8.04613834530271E-4 20 5.811099916051956E-4 21 0.003173754569536069 22 0.0018774322805706323 23 9.387161402853162E-4 24 8.94015371700301E-5 25 4.023069172651355E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2237098.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.26586109198647 #Duplication Level Percentage of deduplicated Percentage of total 1 84.1632179507533 63.3461707133598 2 11.016929796402964 16.583974154324643 3 2.692643675753643 6.079924346084686 4 0.956210650309558 2.8788007192350893 5 0.3974345077282301 1.4956625225917493 6 0.22494949245503412 1.015861035110007 7 0.12175178992021723 0.6414627315485051 8 0.0855937351181928 0.5153828942200146 9 0.06006142701853778 0.4068517520667373 >10 0.25677031403414663 3.2118489009183753 >50 0.013199775063737939 0.6853719021097129 >100 0.00913610783441414 1.3431135429738372 >500 0.0013805109995866296 0.7291444934658069 >1k 7.202666084790695E-4 1.066430291991158 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3224 0.1441152779180885 No Hit GTCCTACAGTGGACATTTCTAAATT 2823 0.1261902697154975 No Hit CTGTAGGACGTGGAATATGGCAAGA 2566 0.11470217218914863 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2414 0.10790765536422633 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.470076858501505E-5 2 0.0 0.0 0.0 0.0 4.470076858501505E-5 3 0.0 0.0 0.0 0.0 4.470076858501505E-5 4 0.0 0.0 0.0 8.94015371700301E-5 4.470076858501505E-5 5 0.0 0.0 0.0 8.94015371700301E-5 4.470076858501505E-5 6 0.0 4.470076858501505E-5 0.0 8.94015371700301E-5 4.470076858501505E-5 7 0.0 4.470076858501505E-5 0.0 1.3410230575504515E-4 4.470076858501505E-5 8 0.0 4.470076858501505E-5 0.0 1.788030743400602E-4 4.470076858501505E-5 9 0.0 4.470076858501505E-5 0.0 3.1290538009510533E-4 4.470076858501505E-5 10 0.0 4.470076858501505E-5 0.0 3.1290538009510533E-4 4.470076858501505E-5 11 0.0 4.470076858501505E-5 0.0 3.1290538009510533E-4 4.470076858501505E-5 12 0.0 4.470076858501505E-5 0.0 3.1290538009510533E-4 4.470076858501505E-5 13 0.0 4.470076858501505E-5 0.0 3.576061486801204E-4 8.94015371700301E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAG 50 6.632138E-5 15.694498 1 CGCCCAC 70 5.2182986E-6 14.012945 1 TAGGACC 700 0.0 12.169098 4 GGTATCA 1025 0.0 11.579478 1 AGGACGG 95 1.4178791E-5 10.959304 5 GGCGAGG 670 0.0 10.453952 19 GCGCCAC 130 4.043668E-7 10.192919 13 GTAGGAC 1755 0.0 10.192919 3 GTCTATA 195 8.913048E-11 10.060576 1 GGACGTG 1600 0.0 10.056406 6 CGTGCGC 85 6.8239134E-4 10.021609 10 AGGACGT 1655 0.0 10.008153 5 TGTAGGA 1750 0.0 9.977217 2 GTATCAA 1920 0.0 9.91124 1 TAGGACG 1735 0.0 9.819444 4 AGGACCT 1345 0.0 9.64078 5 TGGCGAG 1555 0.0 9.556367 18 TAGACTG 380 0.0 9.464853 5 TGGACCG 90 0.0011523163 9.464853 5 TCTAGAC 320 0.0 9.464853 3 >>END_MODULE