FastQCFastQC Report
Thu 26 May 2016
SRR1512023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237098
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39450.17634453206788436No Hit
GTCCTACAGTGGACATTTCTAAATT33260.14867475631376006No Hit
CTGTAGGACGTGGAATATGGCAAGA30670.13709725725024116No Hit
CTTTAGGACGTGAAATATGGCGAGG26660.11917224904765013No Hit
GTCCTAAAGTGTGTATTTCTCATTT25950.11599849447811407No Hit
GGTATCAACGCAGAGTACTTTTTTT24790.1108132053222523No Hit
TATCAACGCAGAGTACTTTTTTTTT23690.10589612077790067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCT400.00530000914.2419038
TAGGACC7800.013.5257584
GGTATCA10450.012.3349531
AGGACCT13650.011.0712775
GTCCTAG3450.010.7935821
GACGTGA8900.010.7747747
GTAGGAC19150.010.770213
GGACCTG12800.010.6885946
TGGCGAG17350.010.67235918
GGCGAGG8650.010.64926819
GTGCGCC1701.4006218E-1010.61161311
TTAGGAC11300.010.5980353
AGGACGT16800.010.5795115
GGACGTG16200.010.5566366
TGTAGGA19500.010.4306752
ACGTGAA10300.010.1398668
GCACCGT750.002646098410.1343716
CTGTAGG19450.010.1127131
GTATTAG1701.4297257E-910.1098251
CGTGAAA10300.010.0476869
Produced by FastQC (version 0.11.5)