FastQCFastQC Report
Thu 26 May 2016
SRR1512022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050825
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35860.17485646020503942No Hit
GGTATCAACGCAGAGTACTTTTTTT20540.10015481574488316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4550.012.2730464
GTATTAG1455.638867E-1112.176311
GGTATCA9050.011.271871
GTAGGAC12050.011.1535483
TGTAGGA12650.011.0880892
GGACCGT600.006041323811.04227356
GTCCTAC10150.010.8233871
GTATCAA18800.010.695651
CACGTTT1002.4983434E-510.41179518
CTGTAGG12600.010.1979271
GGTTAGA1354.158319E-710.1719791
AGGACGT10900.010.0726385
TGGCGAG10200.010.02204918
TAGGACG11400.09.879934
TCCTACA11650.09.8507912
GGACGTG10500.09.825376
TGGAATA15350.09.80393611
TTAGGAC7300.09.5944613
GACGTGG5750.09.547117
GGCGAGG4300.09.46526819
Produced by FastQC (version 0.11.5)