FastQCFastQC Report
Thu 26 May 2016
SRR1512022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050825
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45490.2218131727475528No Hit
GGTATCAACGCAGAGTACTTTTTTT27260.13292211670912926No Hit
TATCAACGCAGAGTACTTTTTTTTT26700.13019150829544207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC905.4200063E-712.66430956
GGTATCA10300.012.6025131
CCGGAAC600.00586568911.0861373
AGAACCG1002.3927096E-510.4526435
GACCGCT750.002657650310.1289777
TAGGACC3850.010.1194424
TTAGGAC5650.010.0910473
GCGTGCG856.620976E-410.05459
TGGACCG1054.0945502E-59.9548985
GTCCTAA6000.09.8627021
TAGGACA4650.09.8089324
GTAGGAC11350.09.7954283
AGGACGT10000.09.7874755
GTCTTAA2450.09.7393381
GTATCAA20200.09.6390511
GGACGTG10000.09.5932146
TAGGACG10500.09.5929014
TTAGACT1606.6102075E-89.5024034
AGGACGG900.00111125889.5024035
ATAACAC1201.6955939E-59.5024033
Produced by FastQC (version 0.11.5)