FastQCFastQC Report
Thu 26 May 2016
SRR1512021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213535
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38040.17185181169486816No Hit
GTATCAACGCAGAGTACTTTTTTTT35960.16245507751176286No Hit
CTGTAGGACGTGGAATATGGCAAGA33610.15183857494911984No Hit
GTCCTAAAGTGTGTATTTCTCATTT30000.1355298199486342No Hit
CTTTAGGACGTGAAATATGGCGAGG28470.12861779913125385No Hit
GTCCTACAGTGTGCATTTCTCATTT24750.1118121014576232No Hit
GGTATCAACGCAGAGTACTTTTTTT22500.10164736496147564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT250.00502477819.6107081
GACCGCC400.00540954514.1980397
TACACGA400.00540954514.1980395
TCGAACT1101.9826984E-1013.767796519
TAGGACC9600.012.9162724
GGTATCA11350.012.1811011
CAGTCGA550.003154264312.0468229
CGGTAGG1251.1481461E-811.7664241
GGCGAGG10200.011.50690819
TGTAGGA22550.011.175062
AGGACCT15800.011.1427655
GTAGGAC23950.010.9866833
GTATAGG2250.010.8948371
GTGTAGG3250.010.8613151
GGACCTG14500.010.8362056
CCTACCG700.001538277310.8173093
CTGTAGG21850.010.72530651
GACGTGG9000.010.6222377
TATGTCG909.877374E-510.51706716
CGTCTAT750.00203629510.4590441
Produced by FastQC (version 0.11.5)