FastQCFastQC Report
Thu 26 May 2016
SRR1512021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213535
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47660.2153117072917302No Hit
GTCCTACAGTGGACATTTCTAAATT42860.19362693609994872No Hit
CTGTAGGACGTGGAATATGGCAAGA38140.17230357776136362No Hit
GTCCTAAAGTGTGTATTTCTCATTT32970.148947272123549No Hit
CTTTAGGACGTGAAATATGGCGAGG32240.1456493798381322No Hit
GTCCTACAGTGTGCATTTCTCATTT29430.13295475336961016No Hit
TATCAACGCAGAGTACTTTTTTTTT29100.13146392535017518No Hit
GGTATCAACGCAGAGTACTTTTTTT28500.12875332895120248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7400.013.4851044
ATGCGGA604.110732E-412.66026711
GTCGAGG853.9577917E-612.29068219
TAAGACG550.00306000412.095734
GGTATCA11700.011.3413371
GGCGAGG9300.011.33554219
AGGACGT20100.011.3005235
GGACGTG20150.011.2197366
GACGTGA11550.011.1804957
TTAGGAC12350.010.927433
GTAGGAC22100.010.9229043
TGTAGGA22800.010.9210192
GGCGTGC1053.4888362E-610.8516588
ACCGTCC803.789692E-410.68218
ACGTGAA12550.010.5922568
TAGGACG21750.010.5306344
AGGACCT14400.010.2297715
CGTGAAA13100.010.1475429
TATGACG750.002639588710.1373734
GTCCTAC24250.010.1171171
Produced by FastQC (version 0.11.5)