FastQCFastQC Report
Thu 26 May 2016
SRR1512020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001292
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32690.1633444794662648No Hit
GTCCTACAGTGGACATTTCTAAATT30240.1511023878574441No Hit
CTGTAGGACGTGGAATATGGCAAGA30010.14995313027784052No Hit
GTCCTAAAGTGTGTATTTCTCATTT26720.13351375011742414No Hit
CTTTAGGACGTGAAATATGGCGAGG23340.1166246604693368No Hit
GGTATCAACGCAGAGTACTTTTTTT21040.10513208467330104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAG350.002224826316.22553610
ACGGTCC508.995485E-515.1438368
CGGTAGG652.421817E-615.089171
CGTTAGG603.126301E-413.0772821
CGCTAGA450.00932045513.0772811
AGGCGAG604.2209658E-412.62049418
CGAGCAA604.2227452E-412.619863510
CGGTCCA604.2227452E-412.61986359
GGTATCA10400.012.5428731
TAGTACC1201.058288E-811.8311224
TAGGACC6700.011.7251714
CGTAGGA1103.2888238E-711.5912282
CCTATAC1752.1827873E-1110.8170253
CGTGCGC700.001538503710.81702410
GTAGGAC19900.010.4161433
CGAAATC1106.3198877E-610.32534313
CCGTAGG1052.8902554E-510.2750061
TAGAAAT6500.010.1929664
TGTAGGA18550.010.1516362
GACGTGG10350.010.150767
Produced by FastQC (version 0.11.5)