##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512020_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2001292 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.261607501553996 31.0 30.0 34.0 23.0 34.0 2 26.333058344309578 31.0 25.0 34.0 10.0 34.0 3 28.291341793201592 31.0 27.0 34.0 16.0 34.0 4 32.7762285563526 35.0 33.0 37.0 26.0 37.0 5 33.2315624106827 35.0 35.0 37.0 27.0 37.0 6 33.51282071781629 37.0 35.0 37.0 28.0 37.0 7 33.57964604865257 37.0 35.0 37.0 28.0 37.0 8 33.61359861529452 37.0 35.0 37.0 28.0 37.0 9 35.215508781327266 39.0 35.0 39.0 29.0 39.0 10 35.12702594124196 39.0 35.0 39.0 28.0 39.0 11 35.12987809874821 39.0 35.0 39.0 28.0 39.0 12 35.026674268422596 39.0 35.0 39.0 28.0 39.0 13 35.02741828778609 39.0 35.0 39.0 28.0 39.0 14 36.199297753651145 40.0 37.0 41.0 27.0 41.0 15 36.14302610513608 40.0 36.0 41.0 27.0 41.0 16 36.08985245531387 40.0 36.0 41.0 27.0 41.0 17 36.04989127023943 40.0 36.0 41.0 27.0 41.0 18 36.00123820012272 40.0 36.0 41.0 27.0 41.0 19 36.029580890744576 40.0 37.0 41.0 27.0 41.0 20 35.93523533797167 40.0 36.0 41.0 27.0 41.0 21 35.8702453215223 40.0 36.0 41.0 27.0 41.0 22 35.766281482162526 40.0 36.0 41.0 27.0 41.0 23 35.66274936391091 40.0 36.0 41.0 27.0 41.0 24 35.570315576137816 40.0 36.0 41.0 27.0 41.0 25 35.46874519060687 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1430517267908407 1101 2 3.9568152656386673 1101 3 2.1595813074542 1101 4 1.2174490972061918 1101 5 0.7532236977114337 1101 6 0.6165945033909566 1101 7 0.21465620943096297 1101 8 0.22181938658111733 1101 9 0.08908874619292817 1101 10 0.27960182075212714 1101 11 0.42578066447118346 1101 12 0.1687294121567504 1101 13 0.41073023787357243 1101 14 0.2000367398346441 1101 15 0.3100350417468434 1101 16 0.33537507221385 1101 17 0.3748954029334186 1101 18 0.5180846767791749 1101 19 0.36173753367341277 1101 20 0.4960654599252905 1101 21 0.3831782798378427 1101 22 0.4824997324170681 1101 23 0.45997394937973723 1101 24 0.34887008030781885 1101 25 0.21275547233133807 1102 1 1.6820359906860247 1102 2 4.399167629756274 1102 3 2.7379733928197147 1102 4 1.839041895801941 1102 5 1.1969482279573924 1102 6 1.1115349492832465 1102 7 1.0010847830630354 1102 8 0.9477239581031185 1102 9 0.8924164341485294 1102 10 0.8016351439052656 1102 11 1.051192576526688 1102 12 0.9393950422950468 1102 13 1.058052385338243 1102 14 1.0654728375521216 1102 15 1.0071907585401476 1102 16 1.1497198844819039 1102 17 1.0570088102427917 1102 18 1.215783953985735 1102 19 1.2294132730482232 1102 20 1.1492156115484136 1102 21 1.2121313553347477 1102 22 1.1980078626864668 1102 23 1.213334491582792 1102 24 1.3245829249303611 1102 25 1.2729169980023798 1103 1 1.6087910016103173 1103 2 4.312769261275225 1103 3 2.68815061567523 1103 4 1.8387958024230144 1103 5 1.4288925734499358 1103 6 1.2794620652707138 1103 7 1.065164352266038 1103 8 0.9993382594235314 1103 9 1.0042526647935404 1103 10 1.0298123905092424 1103 11 1.0463882638070103 1103 12 0.761142988053578 1103 13 0.9036939498865522 1103 14 1.077761476290604 1103 15 1.1961385238104043 1103 16 0.9416753917489302 1103 17 1.1072733086753956 1103 18 1.11331530194812 1103 19 1.0563570842004992 1103 20 1.1705921908207557 1103 21 1.2674397290404542 1103 22 1.1357456441056755 1103 23 1.2338751251916094 1103 24 1.3546912178913217 1103 25 1.3778216479501353 1104 1 1.5257057021547809 1104 2 3.9940045153587107 1104 3 2.3082039278349384 1104 4 1.6732161744738292 1104 5 1.223514851126886 1104 6 0.9495732267952093 1104 7 0.778956321413041 1104 8 0.8580791850133664 1104 9 0.8348334270439892 1104 10 0.7669142507633211 1104 11 0.7398456140792433 1104 12 0.8274304199955012 1104 13 0.8204838772912666 1104 14 0.9421308047842558 1104 15 0.7944359376035095 1104 16 0.7780626422026486 1104 17 0.7786356045011615 1104 18 0.7620040496795113 1104 19 0.899229135017201 1104 20 0.8259982706755693 1104 21 0.6988893660640514 1104 22 1.0331492284976918 1104 23 1.0624935462442409 1104 24 1.0697547387624624 1104 25 0.8797431543022185 1105 1 1.4113520997020466 1105 2 4.201061234827286 1105 3 2.5839934015191517 1105 4 1.5288625720210973 1105 5 1.1289670739168685 1105 6 0.8917551378836137 1105 7 0.5849961783369082 1105 8 0.5574557818452561 1105 9 0.6794680719085804 1105 10 0.5470215578660316 1105 11 0.7592426503592691 1105 12 0.6328570934753159 1105 13 0.714152758231485 1105 14 0.6731936560872711 1105 15 0.6930409452683008 1105 16 0.7082210479256972 1105 17 0.8054828196263557 1105 18 0.6901185105890661 1105 19 0.7635520782977281 1105 20 0.7389415512069917 1105 21 0.7880206895183193 1105 22 0.8743500460756195 1105 23 0.6989524118548687 1105 24 0.740040891036351 1105 25 0.8568100934948646 1106 1 0.5529470068553799 1106 2 2.1939617826276034 1106 3 1.2687718945142272 1106 4 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1107 16 0.5144522142636418 1107 17 0.4782224220122302 1107 18 0.6474344481054857 1107 19 0.5623476790408048 1107 20 0.6171787920725151 1107 21 0.7110241422568677 1107 22 0.7025392924426015 1107 23 0.7575009245029705 1107 24 0.7410533122991083 1107 25 0.6177067548924811 1108 1 1.1522219214780165 1108 2 3.366584612324772 1108 3 2.038009495886122 1108 4 1.2611354027439319 1108 5 0.9340896499473033 1108 6 0.774377045458678 1108 7 0.6069625192438011 1108 8 0.49569002122069605 1108 9 0.46281113484626246 1108 10 0.48944486504831985 1108 11 0.512073334377142 1108 12 0.4588954072499618 1108 13 0.5803884107045718 1108 14 0.5262358680121224 1108 15 0.6202004200346565 1108 16 0.636853065554476 1108 17 0.6678263877270325 1108 18 0.520929425797874 1108 19 0.6203757337076539 1108 20 0.6297984406160921 1108 21 0.5535117482302923 1108 22 0.591140431576612 1108 23 0.605580965647448 1108 24 0.6955482110471607 1108 25 0.5714320631841119 1109 1 0.8363535901790442 1109 2 2.8276119041661083 1109 3 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-1.444548627790482 2315 16 -1.363450889799573 2315 17 -1.2569118750832544 2315 18 -1.241752417035606 2315 19 -1.2718786506940347 2315 20 -1.2812525737384703 2315 21 -1.2351347478356445 2315 22 -1.5727462779369574 2315 23 -1.4204963207872723 2315 24 -1.4171301216420034 2315 25 -1.4291311028271991 2316 1 -0.7984605393504935 2316 2 -0.20465408344539426 2316 3 -0.20109261713352922 2316 4 -0.5327694176569437 2316 5 -0.8674994138881118 2316 6 -0.9105102408891241 2316 7 -1.1892063691442942 2316 8 -1.3513402145907207 2316 9 -1.2252602445137342 2316 10 -1.4506872267575446 2316 11 -1.4078191274566407 2316 12 -1.36404608048408 2316 13 -1.5655566116783461 2316 14 -1.6804856724304997 2316 15 -1.5464752682902514 2316 16 -1.587016437816125 2316 17 -1.6048826910438265 2316 18 -1.7157257925686054 2316 19 -1.5378713323202007 2316 20 -1.5285130658277666 2316 21 -1.6270633695944028 2316 22 -1.7837853238583818 2316 23 -1.9415441264563142 2316 24 -1.907903880449389 2316 25 -1.7470367073332582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74352.0 3 4.0 4 667.0 5 3074.0 6 3540.0 7 3184.0 8 2923.0 9 2868.0 10 2816.0 11 2784.0 12 2681.0 13 2666.0 14 2717.0 15 2870.0 16 2920.0 17 3300.0 18 3706.0 19 4618.0 20 5976.0 21 7761.0 22 9420.0 23 10924.0 24 12876.0 25 14766.0 26 16592.0 27 18756.0 28 21623.0 29 26111.0 30 32693.0 31 40920.0 32 52732.0 33 70367.0 34 96705.0 35 139977.0 36 224954.0 37 311879.0 38 764570.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40510630132934 19.26485490373219 12.990508131746893 25.339530663191578 2 16.925931928435435 21.70667185835796 39.09416033109459 22.273235882112015 3 18.241016303467962 24.961474887222852 30.005516436382095 26.791992372927087 4 12.071202003505736 17.33235329976835 39.02598921096971 31.5704554857562 5 12.99020832542178 37.879180049687896 36.152195681589696 12.978415943300629 6 30.90120327328709 37.86790626650497 18.60898789831164 12.621902561896295 7 27.481546920689237 31.753337344075728 22.977206724456 17.78790901077904 8 24.61774693547968 37.293208587252636 21.014724487980764 17.07431998928692 9 26.167146023668707 15.659483973353213 20.769532881758384 37.40383712121969 10 14.58454296978449 29.344672686117455 34.955153925717916 21.115630418380142 11 33.59025069804906 22.803568894494155 25.2048176877737 18.401362719683085 12 22.873024026478895 24.916304067572348 32.3201212017037 19.890550704245058 13 28.65973581066631 22.311186973215303 26.031133887508666 22.99794332860972 14 20.574658770434297 22.433358050699248 28.13182683986145 28.860156339005005 15 22.469284841992074 31.252810684297945 23.70088922556029 22.577015248149696 16 21.76219162421076 28.424487780893543 28.167703663433425 21.645616931462275 17 21.429056829288278 28.876046074236044 28.14276977072811 21.552127325747566 18 21.143404790719565 27.838899587565198 30.135722730284424 20.881972891430813 19 23.150322218618975 27.26508671196099 27.91172254171203 21.672868527708005 20 23.22354048700734 27.56845105442306 28.46462571029399 20.743382748275607 21 22.717226678072933 27.006269615972904 27.500463462884746 22.776040243069417 22 22.815046667935864 28.323606994968685 27.747471976502887 21.113874360592565 23 22.008425611821238 27.975759501798596 28.1836765388534 21.832138347526755 24 22.637837276038315 27.213133922053313 28.745145008896756 21.40388379301161 25 22.471173506608757 27.595383763623754 28.272898798971056 21.66054393079643 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 395.5 3 544.0 4 544.0 5 544.0 6 1343.5 7 2143.0 8 2143.0 9 2143.0 10 3204.0 11 4265.0 12 4265.0 13 4265.0 14 7365.5 15 10466.0 16 10466.0 17 10466.0 18 18325.0 19 26184.0 20 26184.0 21 26184.0 22 40941.5 23 55699.0 24 55699.0 25 55699.0 26 78772.5 27 101846.0 28 101846.0 29 101846.0 30 130126.0 31 158406.0 32 158406.0 33 158406.0 34 179991.0 35 201576.0 36 201576.0 37 201576.0 38 216252.5 39 230929.0 40 230929.0 41 230929.0 42 240986.0 43 251043.0 44 251043.0 45 251043.0 46 253944.0 47 256845.0 48 256845.0 49 256845.0 50 246726.0 51 236607.0 52 236607.0 53 236607.0 54 209896.0 55 183185.0 56 183185.0 57 183185.0 58 156155.5 59 129126.0 60 129126.0 61 129126.0 62 104286.0 63 79446.0 64 79446.0 65 79446.0 66 60813.5 67 42181.0 68 42181.0 69 42181.0 70 30673.5 71 19166.0 72 19166.0 73 19166.0 74 13294.0 75 7422.0 76 7422.0 77 7422.0 78 5048.5 79 2675.0 80 2675.0 81 2675.0 82 1766.5 83 858.0 84 858.0 85 858.0 86 583.5 87 309.0 88 309.0 89 309.0 90 197.5 91 86.0 92 86.0 93 86.0 94 57.5 95 29.0 96 29.0 97 29.0 98 19.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.49779042738391 3 0.0 4 0.0 5 0.0 6 4.99677208523294E-5 7 0.0 8 0.0 9 0.0 10 9.99354417046588E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.998063251139764E-4 19 5.496449293756233E-4 20 3.997417668186352E-4 21 0.002648289205173458 22 0.001249193021308235 23 6.495803710802821E-4 24 4.99677208523294E-5 25 3.997417668186352E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2001292.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.10407925698233 #Duplication Level Percentage of deduplicated Percentage of total 1 84.13818857277113 63.191211831083294 2 11.201387629990938 16.82539808702042 3 2.642818342670995 5.954593148093073 4 0.9131570397588878 2.7432727475249155 5 0.4003342106482029 1.5033366142902047 6 0.19827824559883828 0.8934903043434336 7 0.11776496355686535 0.6191240409669317 8 0.07767428283342458 0.4666924395320625 9 0.05393939051930011 0.3645961434571353 >10 0.2287906357120771 2.9812396333654116 >50 0.014201073838195457 0.7312484824905967 >100 0.010841377124922754 1.5382407928239539 >500 0.0016822019078658018 0.8324304772117014 >1k 9.42033068404767E-4 1.3551252577968824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3269 0.1633444794662648 No Hit GTCCTACAGTGGACATTTCTAAATT 3024 0.1511023878574441 No Hit CTGTAGGACGTGGAATATGGCAAGA 3001 0.14995313027784052 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2672 0.13351375011742414 No Hit CTTTAGGACGTGAAATATGGCGAGG 2334 0.1166246604693368 No Hit GGTATCAACGCAGAGTACTTTTTTT 2104 0.10513208467330104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.99677208523294E-5 0.0 4 0.0 0.0 0.0 4.99677208523294E-5 0.0 5 0.0 0.0 0.0 4.99677208523294E-5 0.0 6 0.0 0.0 0.0 4.99677208523294E-5 0.0 7 0.0 0.0 0.0 4.99677208523294E-5 0.0 8 0.0 0.0 0.0 4.99677208523294E-5 0.0 9 0.0 0.0 0.0 1.499031625569882E-4 0.0 10 0.0 0.0 0.0 1.499031625569882E-4 0.0 11 0.0 0.0 0.0 1.499031625569882E-4 0.0 12 0.0 0.0 0.0 2.4983860426164696E-4 4.99677208523294E-5 13 0.0 0.0 0.0 2.4983860426164696E-4 4.99677208523294E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCAG 35 0.0022248263 16.225536 10 ACGGTCC 50 8.995485E-5 15.143836 8 CGGTAGG 65 2.421817E-6 15.08917 1 CGTTAGG 60 3.126301E-4 13.077282 1 CGCTAGA 45 0.009320455 13.077281 1 AGGCGAG 60 4.2209658E-4 12.620494 18 CGAGCAA 60 4.2227452E-4 12.6198635 10 CGGTCCA 60 4.2227452E-4 12.6198635 9 GGTATCA 1040 0.0 12.542873 1 TAGTACC 120 1.058288E-8 11.831122 4 TAGGACC 670 0.0 11.725171 4 CGTAGGA 110 3.2888238E-7 11.591228 2 CCTATAC 175 2.1827873E-11 10.817025 3 CGTGCGC 70 0.0015385037 10.817024 10 GTAGGAC 1990 0.0 10.416143 3 CGAAATC 110 6.3198877E-6 10.325343 13 CCGTAGG 105 2.8902554E-5 10.275006 1 TAGAAAT 650 0.0 10.192966 4 TGTAGGA 1855 0.0 10.151636 2 GACGTGG 1035 0.0 10.15076 7 >>END_MODULE