FastQCFastQC Report
Thu 26 May 2016
SRR1512020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001292
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41560.20766584786228096No Hit
GTCCTACAGTGGACATTTCTAAATT35570.17773518307173566No Hit
CTGTAGGACGTGGAATATGGCAAGA34120.17048986354814788No Hit
GTCCTAAAGTGTGTATTTCTCATTT28590.14285771391680974No Hit
CTTTAGGACGTGAAATATGGCGAGG28550.14265784303340043No Hit
GGTATCAACGCAGAGTACTTTTTTT27010.1349628140221417No Hit
TATCAACGCAGAGTACTTTTTTTTT26940.13461303997617538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.012.1549631
GGCGAGG8900.011.63076119
GTATAAC1501.6189006E-1011.4526751
GGACGTG17200.011.324096
GTAGGAC17700.011.1146153
AGGACGT17550.011.1013065
TGTAGGA18750.011.0497522
TAGGACC6050.010.8390464
GTATTAG1855.456968E-1210.8336121
TAGGACG19050.010.7260754
GACGTGA9250.010.6757447
CTGTAGG19450.010.5988781
ACGTGAA10500.010.4899938
GACGTGG9250.010.4704417
TAGGACA6450.010.4615494
GTCCTAC17300.010.3162351
TTAGGAC12050.010.2530633
CAGAACG750.002639367210.1373874
CGTGAAA11200.010.0039279
AGGACCT11600.09.9953775
Produced by FastQC (version 0.11.5)