FastQCFastQC Report
Thu 26 May 2016
SRR1512019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954618
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26170.2741410700405817No Hit
GGTATCAACGCAGAGTACTTTTTTT17860.18709054302349212No Hit
TATCAACGCAGAGTACTTTTTTTTT16100.16865384897414465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATA751.0662805E-513.074142
GTGGTAT2450.012.8073221
GGTATCA7250.012.4429751
TTAGACT907.763687E-611.5684434
CTGTTAC700.001144080811.2064071
ACTAAAC600.006035971511.0426053
GTATTAA1251.4441866E-710.9822781
GTCTAGA906.891994E-510.8951171
CTTAGAC909.866739E-510.5167673
GTCTTAA951.19255405E-410.321691
GTTTAGG1251.7102175E-610.1978291
TGGTATC2950.09.9718022
ATAAGGC951.704458E-49.9632523
GTGTAAC800.0034740229.8056061
GTCTAAG1104.825626E-59.8056051
GTCTTAT908.325269E-49.8056051
TTTAGAC800.00465187349.465093
CCAATAG800.00465187349.465093
TGTTACA1053.2681762E-49.3386712
GCTTATA850.00572838739.2288061
Produced by FastQC (version 0.11.5)