##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512019_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 954618 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.707199110010496 31.0 30.0 34.0 25.0 34.0 2 26.724184961942893 31.0 25.0 34.0 10.0 34.0 3 28.708205795407167 31.0 28.0 34.0 16.0 34.0 4 33.27852607011391 35.0 33.0 37.0 28.0 37.0 5 33.73377413792742 35.0 35.0 37.0 28.0 37.0 6 34.01774741310137 37.0 35.0 37.0 30.0 37.0 7 34.09045607771905 37.0 35.0 37.0 30.0 37.0 8 34.141672375756585 37.0 35.0 37.0 30.0 37.0 9 35.7823925381671 39.0 37.0 39.0 30.0 39.0 10 35.70764221919134 39.0 37.0 39.0 30.0 39.0 11 35.72444894188042 39.0 37.0 39.0 30.0 39.0 12 35.62194406558435 39.0 37.0 39.0 30.0 39.0 13 35.613611936921366 39.0 37.0 39.0 30.0 39.0 14 36.83891252836213 40.0 37.0 41.0 30.0 41.0 15 36.76439266806199 40.0 37.0 41.0 30.0 41.0 16 36.71232681554297 40.0 37.0 41.0 30.0 41.0 17 36.674670915486615 40.0 37.0 41.0 30.0 41.0 18 36.61693787462629 40.0 37.0 41.0 30.0 41.0 19 36.647364705044325 40.0 37.0 41.0 30.0 41.0 20 36.5522407916046 40.0 37.0 41.0 30.0 41.0 21 36.503047292215314 40.0 37.0 41.0 30.0 41.0 22 36.40289099933167 40.0 37.0 41.0 30.0 41.0 23 36.30492092124808 40.0 37.0 41.0 30.0 41.0 24 36.19868575702532 40.0 37.0 41.0 29.0 41.0 25 36.09975089512245 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0039855849705113 1101 2 3.929823546342586 1101 3 2.1399950536081356 1101 4 1.2021427351578353 1101 5 0.8594081262303135 1101 6 0.5199455389533156 1101 7 0.16252860494911658 1101 8 0.1802375024760181 1101 9 0.09701562640719885 1101 10 0.00487170737312681 1101 11 0.22208597692362275 1101 12 0.09228279716425902 1101 13 0.268843893678941 1101 14 -0.20414412194763543 1101 15 0.1203502316223819 1101 16 0.2994762140946605 1101 17 0.2631157778536064 1101 18 0.3650232074547404 1101 19 0.3445904608222605 1101 20 0.25786421801593207 1101 21 0.3669442673247829 1101 22 0.13771394874164145 1101 23 0.2054807872355937 1101 24 0.31150260286198517 1101 25 0.2845545410167105 1102 1 1.4774873129978943 1102 2 4.187019030134689 1102 3 2.3467290818566617 1102 4 1.537017494845344 1102 5 1.068805039117727 1102 6 0.8146839456120532 1102 7 0.678555920005735 1102 8 0.6730267386990292 1102 9 0.6173116136053522 1102 10 0.4051503587338843 1102 11 0.6415201729741682 1102 12 0.6235484244215286 1102 13 0.7210494900428657 1102 14 0.7977269816562185 1102 15 0.6481361895940836 1102 16 0.8660455518879004 1102 17 0.7185982364309567 1102 18 0.6903952056322566 1102 19 0.7581640881239835 1102 20 0.6398291129452005 1102 21 0.6671556867520323 1102 22 0.7065475024771786 1102 23 0.6991923789099488 1102 24 0.9381417285134646 1102 25 0.8138948901782612 1103 1 1.2734112459934757 1103 2 4.037415518381646 1103 3 2.5057426337829867 1103 4 1.6052583142543853 1103 5 1.1596092389562003 1103 6 0.9542742935357325 1103 7 0.6919688656616714 1103 8 0.6782830924756311 1103 9 0.6588314327731482 1103 10 0.7569799212332242 1103 11 0.5331060735164499 1103 12 0.5519288014000381 1103 13 0.7027682875716295 1103 14 0.7345078222532564 1103 15 0.8393355665411235 1103 16 0.5403417328040163 1103 17 0.8519410099606475 1103 18 0.7481823187153225 1103 19 0.8919867383017746 1103 20 0.8813657146842928 1103 21 0.9811641514614351 1103 22 1.1755797250140745 1103 23 1.1954438342735543 1103 24 1.240058875056782 1103 25 1.235229209487656 1104 1 1.5164980714096181 1104 2 4.12759441578946 1104 3 2.3720090462341368 1104 4 1.7530007993462675 1104 5 1.4028583295948494 1104 6 1.0820716783612312 1104 7 0.9034410168928559 1104 8 0.9460342317127157 1104 9 1.1184747197256684 1104 10 0.9560928713501937 1104 11 0.9506415352286197 1104 12 1.013492226818883 1104 13 1.0270282905464896 1104 14 0.9744845642380326 1104 15 1.1135232262709565 1104 16 0.9414478407374389 1104 17 0.9098696943953541 1104 18 0.9714727869857072 1104 19 0.9637485329551296 1104 20 1.1290770989418633 1104 21 1.0708968195073538 1104 22 1.1239388179514833 1104 23 1.2347931507313064 1104 24 1.3287368191564113 1104 25 1.2527403314164474 1105 1 1.4313474455963444 1105 2 4.12570223166836 1105 3 2.4677451374521553 1105 4 1.5121153461114147 1105 5 1.1865560575696819 1105 6 0.914042919971024 1105 7 0.8899745636673657 1105 8 0.7499398307990361 1105 9 0.6853681676432117 1105 10 0.7709443518643226 1105 11 0.7447632435069593 1105 12 0.698943823415064 1105 13 0.6661629176329242 1105 14 0.7212232673020367 1105 15 0.6758740280795905 1105 16 0.9838365096321269 1105 17 1.031622439642078 1105 18 0.7624057496054206 1105 19 0.7054741767892097 1105 20 0.7647292647145107 1105 21 0.9483682436655272 1105 22 1.0869692614036168 1105 23 1.1049556187853398 1105 24 1.1283326383305479 1105 25 1.1054916630834413 1106 1 0.253047860107916 1106 2 2.180932230567887 1106 3 1.0249559881179415 1106 4 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1107 16 1.0101550420482255 1107 17 0.9043553977408862 1107 18 0.973162472557263 1107 19 0.9214685482713847 1107 20 1.074636498402235 1107 21 1.185391383127687 1107 22 1.2072788298839114 1107 23 1.200484074069891 1107 24 1.2454891945114923 1107 25 1.2036259136567153 1108 1 1.2740043040807194 1108 2 3.473712687480411 1108 3 2.2183746591434925 1108 4 1.3495506896534408 1108 5 0.8700089041581194 1108 6 0.6096739755223197 1108 7 0.4544222462606129 1108 8 0.5113820000178748 1108 9 0.4896700057187289 1108 10 0.45282392681468764 1108 11 0.5125996914281501 1108 12 0.5166541539618805 1108 13 0.5846467822783055 1108 14 0.468535816832933 1108 15 0.7489400863151374 1108 16 0.6524375441354593 1108 17 0.7255726403220848 1108 18 0.45355665586100713 1108 19 0.6740076788471399 1108 20 0.74658710998213 1108 21 0.7224722138063839 1108 22 0.8784441955754545 1108 23 0.827080909579621 1108 24 0.7413664002925202 1108 25 0.7288672627800779 1109 1 1.1465654027101735 1109 2 3.188841368235785 1109 3 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-0.623637105694236 2315 11 -0.5295647397904943 2315 12 -0.49227918173924223 2315 13 -0.6391153494774002 2315 14 -0.6140755517754073 2315 15 -0.630554170524185 2315 16 -0.7140169515801134 2315 17 -0.6057658294255859 2315 18 -0.6484215486264588 2315 19 -0.5282718269949598 2315 20 -0.5121024701324899 2315 21 -0.601584454187936 2315 22 -0.8220556619342858 2315 23 -0.8334455686616522 2315 24 -0.7832531253544559 2315 25 -0.5679767260337982 2316 1 -0.5925831257480212 2316 2 -0.31801066463074434 2316 3 -0.15056040757752598 2316 4 -0.4627120295780287 2316 5 -0.9084136279172412 2316 6 -0.8197577621055174 2316 7 -0.9175914335538025 2316 8 -0.9379890985092061 2316 9 -0.8401623363355668 2316 10 -1.2213612352688514 2316 11 -1.179476788491698 2316 12 -1.2865462612129548 2316 13 -1.487851650174747 2316 14 -1.6239674615093804 2316 15 -1.2862801970056736 2316 16 -1.461239487168008 2316 17 -1.493608076967753 2316 18 -1.6720075382561461 2316 19 -1.5878029483844642 2316 20 -1.3518974036362934 2316 21 -1.32463605089049 2316 22 -1.5085688212034327 2316 23 -1.2080575175036614 2316 24 -1.28871822268583 2316 25 -1.399381815927967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26114.0 3 0.0 4 264.0 5 1131.0 6 1297.0 7 1161.0 8 1156.0 9 1069.0 10 1040.0 11 1064.0 12 1089.0 13 1096.0 14 1069.0 15 1143.0 16 1163.0 17 1323.0 18 1503.0 19 1903.0 20 2452.0 21 3069.0 22 3756.0 23 4611.0 24 5285.0 25 6289.0 26 6887.0 27 8029.0 28 9466.0 29 11343.0 30 14591.0 31 18874.0 32 24802.0 33 33576.0 34 47104.0 35 68987.0 36 110874.0 37 154372.0 38 375666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93547995114276 18.0500472440285 12.727813638544422 25.286659166284313 2 18.242008438672038 21.39337928879011 36.71050232671204 23.65410994582581 3 18.65992470286544 23.88704172768584 29.728121615138203 27.72491195431052 4 13.236394034053411 16.54640913957206 36.670060694434845 33.54713613193969 5 14.321959150152209 36.65183350827242 34.69136345637732 14.33484388519806 6 32.519429258016565 35.93011647603175 17.585377172206236 13.965077093745451 7 28.518946845754005 31.42911614907743 21.67893335344609 18.37300365172247 8 26.52686889087456 34.367709772610375 20.315582060658883 18.78983927585618 9 26.770813037256787 15.505469203388161 19.774087645529416 37.949630113825634 10 16.045492590222047 28.159565563990586 32.978880535335115 22.816061310452255 11 34.442504166592464 22.844030642655643 23.295625365984474 19.41783982476742 12 23.142660205443434 24.663687464514602 30.373824922639216 21.81982740740275 13 28.85866388440193 21.3072663620422 25.487891491675203 24.34617826188067 14 22.394950016603516 20.783413662233126 26.97102607642646 29.8506102447369 15 24.13551808157818 28.00795710954539 23.602949033016348 24.25357577586008 16 23.92244856057606 27.38802327213608 25.260051664644916 23.42947650264294 17 23.069541952906818 27.13588053022256 26.544649273321895 23.24992824354873 18 22.908212115053832 26.880707804410996 27.287678580033397 22.92340150050177 19 23.8735440374017 26.707060168089765 27.16808661975741 22.25130917475113 20 24.237281743787005 26.147062890472778 27.0284681106041 22.58718725513612 21 24.33164883698517 25.895464849757172 26.822985413602517 22.94990089965514 22 24.30143661965929 25.78760572468945 26.82835359657742 23.082604059073834 23 24.015591723941426 25.942244416300287 26.74718130669206 23.29498255306623 24 23.673368481809984 26.11277611858997 26.870147923198516 23.343707476401534 25 23.73943813939456 25.97824880003855 26.85923315601908 23.423079904547805 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 321.0 1 321.0 2 369.0 3 417.0 4 417.0 5 417.0 6 853.0 7 1289.0 8 1289.0 9 1289.0 10 1747.0 11 2205.0 12 2205.0 13 2205.0 14 3194.5 15 4184.0 16 4184.0 17 4184.0 18 7153.5 19 10123.0 20 10123.0 21 10123.0 22 14620.5 23 19118.0 24 19118.0 25 19118.0 26 26731.5 27 34345.0 28 34345.0 29 34345.0 30 43679.5 31 53014.0 32 53014.0 33 53014.0 34 62816.5 35 72619.0 36 72619.0 37 72619.0 38 82670.5 39 92722.0 40 92722.0 41 92722.0 42 101960.0 43 111198.0 44 111198.0 45 111198.0 46 117538.0 47 123878.0 48 123878.0 49 123878.0 50 125789.5 51 127701.0 52 127701.0 53 127701.0 54 119529.5 55 111358.0 56 111358.0 57 111358.0 58 97717.5 59 84077.0 60 84077.0 61 84077.0 62 69261.0 63 54445.0 64 54445.0 65 54445.0 66 42316.5 67 30188.0 68 30188.0 69 30188.0 70 21922.0 71 13656.0 72 13656.0 73 13656.0 74 9477.5 75 5299.0 76 5299.0 77 5299.0 78 3541.5 79 1784.0 80 1784.0 81 1784.0 82 1123.5 83 463.0 84 463.0 85 463.0 86 310.5 87 158.0 88 158.0 89 158.0 90 99.0 91 40.0 92 40.0 93 40.0 94 26.0 95 12.0 96 12.0 97 12.0 98 8.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4721742099981356 3 0.0 4 0.0 5 0.0 6 1.0475394346220164E-4 7 0.0 8 1.0475394346220164E-4 9 0.0 10 1.0475394346220164E-4 11 1.0475394346220164E-4 12 0.0 13 0.0 14 1.0475394346220164E-4 15 0.0 16 0.0 17 0.0 18 4.1901577384880655E-4 19 9.427854911598147E-4 20 6.285236607732098E-4 21 0.003142618303866049 22 0.0016760630953952262 23 9.427854911598147E-4 24 1.0475394346220164E-4 25 4.1901577384880655E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 954618.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.09980017039412 #Duplication Level Percentage of deduplicated Percentage of total 1 80.28677559184996 58.68947252089508 2 13.2504758296929 19.372142706263766 3 3.684425105026644 8.07992216760693 4 1.2990030141571454 3.7982744302250784 5 0.5756129962394143 2.1038597500291507 6 0.2988756627506162 1.3108650733718492 7 0.17584327501499605 0.8997875785432707 8 0.09759178421617022 0.5707151939579413 9 0.07771154594632772 0.5112628631648062 >10 0.2435114669167111 2.782522630494536 >50 0.005088544404834897 0.25567111354977945 >100 0.0037775654911254442 0.5511101840689206 >500 8.71745528761407E-4 0.43576885245769786 >1k 4.358727643807035E-4 0.6386249353712252 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2617 0.2741410700405817 No Hit GGTATCAACGCAGAGTACTTTTTTT 1786 0.18709054302349212 No Hit TATCAACGCAGAGTACTTTTTTTTT 1610 0.16865384897414465 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.0950788692440328E-4 0.0 10 0.0 0.0 0.0 2.0950788692440328E-4 0.0 11 0.0 0.0 0.0 2.0950788692440328E-4 0.0 12 0.0 0.0 0.0 2.0950788692440328E-4 0.0 13 0.0 0.0 0.0 2.0950788692440328E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTATA 75 1.0662805E-5 13.07414 2 GTGGTAT 245 0.0 12.807322 1 GGTATCA 725 0.0 12.442975 1 TTAGACT 90 7.763687E-6 11.568443 4 CTGTTAC 70 0.0011440808 11.206407 1 ACTAAAC 60 0.0060359715 11.042605 3 GTATTAA 125 1.4441866E-7 10.982278 1 GTCTAGA 90 6.891994E-5 10.895117 1 CTTAGAC 90 9.866739E-5 10.516767 3 GTCTTAA 95 1.19255405E-4 10.32169 1 GTTTAGG 125 1.7102175E-6 10.197829 1 TGGTATC 295 0.0 9.971802 2 ATAAGGC 95 1.704458E-4 9.963252 3 GTGTAAC 80 0.003474022 9.805606 1 GTCTAAG 110 4.825626E-5 9.805605 1 GTCTTAT 90 8.325269E-4 9.805605 1 TTTAGAC 80 0.0046518734 9.46509 3 CCAATAG 80 0.0046518734 9.46509 3 TGTTACA 105 3.2681762E-4 9.338671 2 GCTTATA 85 0.0057283873 9.228806 1 >>END_MODULE