FastQCFastQC Report
Thu 26 May 2016
SRR1512019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954618
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34100.3572109472061076No Hit
GGTATCAACGCAGAGTACTTTTTTT22690.2376866977157355No Hit
TATCAACGCAGAGTACTTTTTTTTT21970.23014441378645698No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11220.11753392456459023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG400.005293952814.2431657
GGTATCA6650.012.6235831
GTCTAAG802.7448132E-511.9242661
TGTTAGA802.8500921E-511.8805052
GTGGTAT2400.010.731841
TTATACT909.460759E-510.5604494
TGGTATC2350.010.5155112
TATACTT1303.8088365E-710.2355125
CTAAAGC750.002635815210.138033
GCCCTAT856.617102E-410.05399911
GTATAAG1159.75586E-69.954171
GAGAGTC1054.1213687E-59.9476077
GTCCTGA1356.0745333E-79.8927251
TATATAC1752.5429472E-99.7759583
GTATCAA14400.09.6719051
GGTTACA800.0043611889.53941251
TATATGG900.00110797639.5044042
ATATGAG1002.739549E-49.5044043
AATACCT1106.7927045E-59.5044035
TGGACAG2054.1654857E-109.2725895
Produced by FastQC (version 0.11.5)