##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512019_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 954618 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46702764875584 33.0 31.0 34.0 28.0 34.0 2 31.647489362237042 34.0 31.0 34.0 28.0 34.0 3 31.7596305537922 34.0 31.0 34.0 28.0 34.0 4 35.3004102164426 37.0 35.0 37.0 33.0 37.0 5 35.004977907393325 37.0 35.0 37.0 32.0 37.0 6 34.95780615911286 37.0 35.0 37.0 32.0 37.0 7 34.84046288672537 37.0 35.0 37.0 32.0 37.0 8 34.77375348044977 37.0 35.0 37.0 32.0 37.0 9 36.36246750008904 39.0 37.0 39.0 32.0 39.0 10 36.21212254535322 39.0 37.0 39.0 32.0 39.0 11 36.21435904204614 39.0 37.0 39.0 32.0 39.0 12 36.06844832173707 39.0 37.0 39.0 32.0 39.0 13 36.04526941666719 39.0 37.0 39.0 32.0 39.0 14 37.19267183313116 40.0 37.0 41.0 31.0 41.0 15 37.119054951823664 40.0 37.0 41.0 31.0 41.0 16 37.02990201316128 40.0 37.0 41.0 31.0 41.0 17 36.95813299141646 40.0 37.0 41.0 31.0 41.0 18 36.884065668152076 40.0 37.0 41.0 31.0 41.0 19 36.905636600189816 40.0 37.0 41.0 31.0 41.0 20 36.80527813219528 40.0 37.0 41.0 31.0 41.0 21 36.70787267786696 40.0 37.0 41.0 30.0 41.0 22 36.598005694424366 40.0 37.0 41.0 30.0 41.0 23 36.68714396753466 40.0 37.0 41.0 31.0 41.0 24 36.60153066462187 40.0 37.0 41.0 30.0 41.0 25 36.15734775585627 39.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15078418999429033 1101 2 0.10593631200455178 1101 3 0.0596255688503291 1101 4 0.20864287935543047 1101 5 0.12274234319013289 1101 6 0.06545166883602604 1101 7 -0.05117566425570175 1101 8 0.11215035750394264 1101 9 0.09306578563048618 1101 10 0.060032901700772356 1101 11 0.016167453789172725 1101 12 0.14855182289528557 1101 13 0.1799474438524129 1101 14 0.018337176877828654 1101 15 0.02721674579943567 1101 16 0.27439826511306364 1101 17 0.21622008305708107 1101 18 0.23282724360483797 1101 19 0.0726267587654732 1101 20 -0.018472700047333035 1101 21 0.1366501123992805 1101 22 0.04856647914972001 1101 23 -0.06859405675702135 1101 24 -0.340479285069776 1101 25 -0.10180593576948382 1102 1 0.21870094463663037 1102 2 0.24401181179896625 1102 3 0.15796199522302956 1102 4 0.12218455759784064 1102 5 0.13257154074089073 1102 6 0.3084437121311723 1102 7 0.28138903953484373 1102 8 0.30384193517627267 1102 9 0.6310434935216236 1102 10 0.5181296267832849 1102 11 0.42713792730201305 1102 12 0.5817185580903725 1102 13 0.5035302947086464 1102 14 0.6656848493193976 1102 15 0.6007148621940246 1102 16 0.6149432882830794 1102 17 0.6656780603204666 1102 18 0.7204810158163824 1102 19 0.6259691860049656 1102 20 0.6795378459204215 1102 21 0.3989768929342503 1102 22 0.6100170220059127 1102 23 0.5363516576239462 1102 24 0.6049544998102618 1102 25 0.5773518870271417 1103 1 0.28447944616279486 1103 2 0.25031576514341936 1103 3 0.2989670614617417 1103 4 0.32683096979877035 1103 5 0.31733191709005837 1103 6 0.44892038960161784 1103 7 0.49979847133991484 1103 8 0.5606650993203104 1103 9 0.5240755797001313 1103 10 0.5895158671726364 1103 11 0.7568734969129736 1103 12 0.7276417044029273 1103 13 0.7541596411323894 1103 14 0.376472633705319 1103 15 0.6643824309239008 1103 16 0.742062087909666 1103 17 0.7752774288762225 1103 18 0.7271468433544825 1103 19 1.0870716436027479 1103 20 0.9976029727861686 1103 21 0.8225899707529365 1103 22 0.8502936197591424 1103 23 0.7643035545789587 1103 24 1.0448230712987723 1103 25 1.1490698454273485 1104 1 0.19969586740331025 1104 2 0.16653282192272556 1104 3 0.13787826181872376 1104 4 0.15120591063957534 1104 5 0.13214673190486792 1104 6 0.21901761351410443 1104 7 0.25095123052480517 1104 8 0.41031284856645556 1104 9 0.48632187126369075 1104 10 0.5032178130030971 1104 11 0.5526356701851896 1104 12 0.6718765562683018 1104 13 0.5389148234779313 1104 14 0.5040130985951805 1104 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-0.8864354857102583 2314 18 -1.0153205657795965 2314 19 -0.9676488593936199 2314 20 -1.0456381624089914 2314 21 -0.8058786232328998 2314 22 -0.9337805436253674 2314 23 -0.9346875352167316 2314 24 -0.7825964227873925 2314 25 -0.8115531841611983 2315 1 -0.5542642246419476 2315 2 -0.5014084318734007 2315 3 -0.9149019081324212 2315 4 -0.7934387331681307 2315 5 -0.41353913439168366 2315 6 -0.49053352186247423 2315 7 -0.5004671192564629 2315 8 -0.43278937759472313 2315 9 -0.5734304389254845 2315 10 -0.5046145806473774 2315 11 -0.5638571342502274 2315 12 -0.5629401103519172 2315 13 -0.6601102845879225 2315 14 -0.9580944107853924 2315 15 -0.7815441803971126 2315 16 -0.8009079003529394 2315 17 -0.6671965046918586 2315 18 -0.37011928699052987 2315 19 -0.5504742992867051 2315 20 -0.7196886885258493 2315 21 -0.6632094997793629 2315 22 -0.9542402687442149 2315 23 -0.6030168076523452 2315 24 -0.6857738221479934 2315 25 -0.7455506598801804 2316 1 -0.7440405651158386 2316 2 -0.7707128018621603 2316 3 -0.8872630558028618 2316 4 -0.730828271768516 2316 5 -0.895320054182605 2316 6 -1.131677599042348 2316 7 -1.2274963708974553 2316 8 -1.18395405334482 2316 9 -1.2569143966921459 2316 10 -1.4755822956266726 2316 11 -1.5813638298300958 2316 12 -1.4908513819062534 2316 13 -1.382336685019112 2316 14 -1.484352365050718 2316 15 -1.4059127485666778 2316 16 -1.4645746129799093 2316 17 -1.506304122820275 2316 18 -1.6108112819599611 2316 19 -1.5254990551748477 2316 20 -1.5884574121247894 2316 21 -1.556289132264098 2316 22 -1.639729766815968 2316 23 -1.8243553386776412 2316 24 -1.7266408840732268 2316 25 -1.7341880205891442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9523.0 3 0.0 4 169.0 5 1573.0 6 2015.0 7 1685.0 8 1320.0 9 1235.0 10 1237.0 11 1198.0 12 1191.0 13 1146.0 14 1098.0 15 1058.0 16 1131.0 17 1196.0 18 1271.0 19 1484.0 20 1754.0 21 2193.0 22 2861.0 23 3645.0 24 4461.0 25 5375.0 26 6266.0 27 7177.0 28 8419.0 29 10290.0 30 13492.0 31 17585.0 32 22876.0 33 30881.0 34 40821.0 35 56466.0 36 85875.0 37 166588.0 38 438063.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.368722180882024 18.500154192510138 13.157725781513948 24.97339784509389 2 16.170342482542754 20.585092675813783 38.27625290954078 24.968311932102687 3 18.723510346546995 24.17406753277227 30.042907215242113 27.059514905438615 4 13.626916735280501 16.93797938023377 36.394034053411936 33.04106983107379 5 14.287076636418988 36.829788950916594 34.71667821171558 14.166456200948824 6 32.67680594815488 36.176608382043604 17.41401157726931 13.732574092532209 7 28.53298387417794 31.585828048496882 21.703341022272784 18.17784705505239 8 26.654012390296433 34.41669861661942 20.17707606602851 18.752212927055638 9 26.84749292387112 15.364889411261887 19.682532698943454 38.10508496592354 10 15.983461447406189 28.242186927126873 32.95443831983055 22.81991330563639 11 34.63406304930349 22.659430264252297 23.273497880827723 19.433008805616485 12 23.159316082453923 24.63173751175863 30.41310765143754 21.79583875434991 13 28.95901816223872 21.19800799901112 25.60144476638823 24.241529072361928 14 22.376804124791278 20.69194169814523 27.07156160893677 29.85969256812673 15 24.158459195196404 28.062952929863044 23.611329348493324 24.167258526447227 16 23.834769509898198 27.432962713881366 25.28927801487087 23.442989761349565 17 23.080017347253037 27.179772432533223 26.551667787533862 23.188542432679878 18 22.878051744257913 27.01551824918449 27.34392186193849 22.762508144619105 19 23.907259238774042 26.68145792348353 27.23853939481552 22.172743442926908 20 24.203817652715536 26.02988839514864 27.11377744815204 22.65251650398379 21 24.316009126163554 25.890146634570062 26.91464020163039 22.879204037635997 22 24.250537911499677 25.772507956062007 26.982206495163506 22.994747637274806 23 24.069523097196992 25.816295104429205 26.795430213970405 23.318751584403394 24 23.631211468858197 26.082957515569593 27.010271479229193 23.275559536343017 25 23.71248074928497 26.031052581953045 26.96325863532074 23.293208033441243 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 338.0 1 338.0 2 359.5 3 381.0 4 381.0 5 381.0 6 835.5 7 1290.0 8 1290.0 9 1290.0 10 1767.0 11 2244.0 12 2244.0 13 2244.0 14 3176.0 15 4108.0 16 4108.0 17 4108.0 18 7137.5 19 10167.0 20 10167.0 21 10167.0 22 14966.0 23 19765.0 24 19765.0 25 19765.0 26 27521.5 27 35278.0 28 35278.0 29 35278.0 30 44108.0 31 52938.0 32 52938.0 33 52938.0 34 62710.5 35 72483.0 36 72483.0 37 72483.0 38 82508.5 39 92534.0 40 92534.0 41 92534.0 42 102017.0 43 111500.0 44 111500.0 45 111500.0 46 117763.0 47 124026.0 48 124026.0 49 124026.0 50 126289.0 51 128552.0 52 128552.0 53 128552.0 54 119789.0 55 111026.0 56 111026.0 57 111026.0 58 97591.0 59 84156.0 60 84156.0 61 84156.0 62 68950.5 63 53745.0 64 53745.0 65 53745.0 66 41721.0 67 29697.0 68 29697.0 69 29697.0 70 21483.5 71 13270.0 72 13270.0 73 13270.0 74 9148.5 75 5027.0 76 5027.0 77 5027.0 78 3278.0 79 1529.0 80 1529.0 81 1529.0 82 971.0 83 413.0 84 413.0 85 413.0 86 267.5 87 122.0 88 122.0 89 122.0 90 73.5 91 25.0 92 25.0 93 25.0 94 14.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47212602318414276 2 0.0 3 0.0 4 0.0 5 0.04033026823294763 6 0.05311024933533623 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.003456880134252654 25 0.011313425893917777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 954618.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.21682371341717 #Duplication Level Percentage of deduplicated Percentage of total 1 78.54070430701881 55.148787886535644 2 13.941606182379044 19.57870607179992 3 4.1242918003462465 8.687840108628128 4 1.5719303009293746 4.415038113205467 5 0.7054287189341881 2.4766481999891794 6 0.36581694488242317 1.541190235811397 7 0.21068321840062 1.035545448406819 8 0.12486690947900968 0.7014206216421472 9 0.08940780934798867 0.5650139148831478 >10 0.31273413239275305 3.4668719002206005 >50 0.006415853993814981 0.3102897038236289 >100 0.004622646284449021 0.6651949178980295 >500 8.947053667786393E-4 0.46531175316772233 >1k 5.964702445190929E-4 0.9421411239881109 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3410 0.3572109472061076 No Hit GGTATCAACGCAGAGTACTTTTTTT 2269 0.2376866977157355 No Hit TATCAACGCAGAGTACTTTTTTTTT 2197 0.23014441378645698 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1122 0.11753392456459023 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTGCG 40 0.0052939528 14.243165 7 GGTATCA 665 0.0 12.623583 1 GTCTAAG 80 2.7448132E-5 11.924266 1 TGTTAGA 80 2.8500921E-5 11.880505 2 GTGGTAT 240 0.0 10.73184 1 TTATACT 90 9.460759E-5 10.560449 4 TGGTATC 235 0.0 10.515511 2 TATACTT 130 3.8088365E-7 10.235512 5 CTAAAGC 75 0.0026358152 10.13803 3 GCCCTAT 85 6.617102E-4 10.053999 11 GTATAAG 115 9.75586E-6 9.95417 1 GAGAGTC 105 4.1213687E-5 9.947607 7 GTCCTGA 135 6.0745333E-7 9.892725 1 TATATAC 175 2.5429472E-9 9.775958 3 GTATCAA 1440 0.0 9.671905 1 GGTTACA 80 0.004361188 9.5394125 1 TATATGG 90 0.0011079763 9.504404 2 ATATGAG 100 2.739549E-4 9.504404 3 AATACCT 110 6.7927045E-5 9.504403 5 TGGACAG 205 4.1654857E-10 9.272589 5 >>END_MODULE