FastQCFastQC Report
Thu 26 May 2016
SRR1512018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597444
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29980.18767481050978937No Hit
GTCCTACAGTGGACATTTCTAAATT23670.14817420829775566No Hit
CTGTAGGACGTGGAATATGGCAAGA21700.13584200760715243No Hit
GTCCTAAAGTGTGTATTTCTCATTT19700.12332200690603239No Hit
GGTATCAACGCAGAGTACTTTTTTT18450.11549700646783237No Hit
TATCAACGCAGAGTACTTTTTTTTT17380.10879880609273314No Hit
CTTTAGGACGTGAAATATGGCGAGG16970.10623220594900354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG603.1953614E-413.0432081
GGTATCA7600.011.7131451
GGCGAGG6100.011.64126319
TAGGACC4200.011.4964134
ACACCGT752.1406563E-411.3611626
GGACGTG13500.011.2209016
AGGACGT13600.011.1383945
GTAGGAC13000.010.9970233
TAGGACG14300.010.8579874
TGTAGGA13300.010.8121332
TGGCGAG12500.010.75590718
GACGTGA7550.010.6589277
CTGTAGG12800.010.6230811
GACGTGG6850.010.5042387
GCGCCAC1002.489657E-510.41472413
CGTGCGC1002.4905168E-510.41439910
TTAGGAC9250.010.2352813
ATTTCTC15450.010.111382515
CCCGTTT750.00272294310.09912712
CTACACG750.0027236510.098814
Produced by FastQC (version 0.11.5)