##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512018_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1597444 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.144008804064494 31.0 30.0 34.0 25.0 34.0 2 27.24195214354932 31.0 26.0 34.0 10.0 34.0 3 29.2045642914556 31.0 28.0 34.0 16.0 34.0 4 33.74662523381102 35.0 33.0 37.0 28.0 37.0 5 34.20155072728684 37.0 35.0 37.0 31.0 37.0 6 34.496341655795135 37.0 35.0 37.0 32.0 37.0 7 34.580515498508866 37.0 35.0 37.0 32.0 37.0 8 34.62412391295094 37.0 35.0 37.0 32.0 37.0 9 36.32287203808083 39.0 37.0 39.0 32.0 39.0 10 36.24239409957407 39.0 37.0 39.0 32.0 39.0 11 36.26445997480976 39.0 37.0 39.0 32.0 39.0 12 36.17346210571388 39.0 37.0 39.0 32.0 39.0 13 36.179787836068115 39.0 37.0 39.0 32.0 39.0 14 37.458617015682556 40.0 38.0 41.0 32.0 41.0 15 37.411345249035335 40.0 38.0 41.0 32.0 41.0 16 37.36022358217252 40.0 38.0 41.0 32.0 41.0 17 37.336105678821916 40.0 38.0 41.0 32.0 41.0 18 37.29070627827955 40.0 38.0 41.0 32.0 41.0 19 37.32664431429208 40.0 38.0 41.0 32.0 41.0 20 37.24317534761782 40.0 38.0 41.0 32.0 41.0 21 37.19205430675504 40.0 38.0 41.0 32.0 41.0 22 37.100548125630695 40.0 38.0 41.0 32.0 41.0 23 37.022633657267484 40.0 38.0 41.0 31.0 41.0 24 36.94893091714013 40.0 38.0 41.0 31.0 41.0 25 36.85720125400327 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8779871473517602 1101 2 3.8990120957463184 1101 3 1.9253358127476083 1101 4 0.9125896152515409 1101 5 0.669136272044689 1101 6 0.3340091664090181 1101 7 -0.156587470099808 1101 8 -0.05883675956358303 1101 9 -0.11985182522656856 1101 10 0.07667487246659022 1101 11 0.053701711948349384 1101 12 -0.12564256268306906 1101 13 0.011561436748358744 1101 14 -0.3110703685725298 1101 15 -0.06547272713086727 1101 16 0.040762556484864376 1101 17 -0.09694622797614727 1101 18 0.025113590848725664 1101 19 -0.17168119647397617 1101 20 -0.14115737588637245 1101 21 -0.024495828978515988 1101 22 0.08306875262032065 1101 23 -0.05415990539994908 1101 24 -0.19731054370596723 1101 25 -0.33591218012675483 1102 1 1.5951783751596231 1102 2 4.3966693665765355 1102 3 2.625871154397643 1102 4 1.6737181882588814 1102 5 1.2625975954794555 1102 6 0.9720831066145763 1102 7 0.8817510530463224 1102 8 0.9086388786533703 1102 9 1.0018782676699942 1102 10 0.8871957823198642 1102 11 0.8843791937948424 1102 12 0.8818544957007006 1102 13 0.8945980623082406 1102 14 1.069438254751077 1102 15 1.1467892619867754 1102 16 1.0649155010264622 1102 17 0.9696797544586673 1102 18 1.1021530572622922 1102 19 1.1260924388436209 1102 20 1.0677152537560204 1102 21 1.0186565434918577 1102 22 1.0633249820208803 1102 23 1.0400798899545691 1102 24 1.0033263730901751 1102 25 0.9959297591946878 1103 1 1.2554472540585095 1103 2 4.250233769519735 1103 3 2.3857322541246333 1103 4 1.4410168837438349 1103 5 1.026726426586059 1103 6 0.9564119024128885 1103 7 0.5768923807639581 1103 8 0.5930182724857715 1103 9 0.6871435211645718 1103 10 0.5300430172397625 1103 11 0.6056226444231427 1103 12 0.5875331398625363 1103 13 0.713788903591194 1103 14 0.5511977422765355 1103 15 0.7527070252068953 1103 16 0.7055063158282024 1103 17 0.8933610530469593 1103 18 0.7961169167100977 1103 19 0.872679041871308 1103 20 0.9826112519401633 1103 21 1.0052015647083508 1103 22 0.8930799362146828 1103 23 0.9998118937628249 1103 24 0.9399960766897237 1103 25 1.029943932949287 1104 1 1.4979899530738372 1104 2 4.025760989199817 1104 3 2.364249583821408 1104 4 1.648856123777513 1104 5 1.2255249091891045 1104 6 1.0255714355048653 1104 7 0.7371772908279581 1104 8 0.7176109571605096 1104 9 0.7340708851009623 1104 10 0.8196213110726873 1104 11 0.6950766273582261 1104 12 0.8336118963824859 1104 13 0.7126806966753918 1104 14 0.7973074219053942 1104 15 0.7893710092938591 1104 16 0.9916420945609872 1104 17 0.8685669043176318 1104 18 0.9230187845431885 1104 19 0.9926452429132553 1104 20 0.9037198299332729 1104 21 0.9694069065208026 1104 22 0.8946593340026041 1104 23 0.9994752184144957 1104 24 0.9884147075536163 1104 25 0.9598165065482149 1105 1 1.2100764536428912 1105 2 4.142992680506531 1105 3 2.400606556392269 1105 4 1.32557284212983 1105 5 0.9267443729518376 1105 6 0.691464235710022 1105 7 0.5917096859900468 1105 8 0.45048292084098307 1105 9 0.4808758491933247 1105 10 0.36197915282493653 1105 11 0.4618795716818198 1105 12 0.4173103199416133 1105 13 0.37472005039477807 1105 14 0.4157277130925081 1105 15 0.26429013344042573 1105 16 0.40953338788247606 1105 17 0.5123057913342635 1105 18 0.41878918449144464 1105 19 0.3959465436216547 1105 20 0.3978982435978011 1105 21 0.3665547206884625 1105 22 0.36298357270295867 1105 23 0.2626317992863747 1105 24 0.41643434696815973 1105 25 0.5011746762292191 1106 1 0.3478572105128599 1106 2 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1107 14 0.3568402829863402 1107 15 0.43055656048166213 1107 16 0.29311697280029136 1107 17 0.3517567558710297 1107 18 0.39400283692053506 1107 19 0.3709347141526109 1107 20 0.5058089187031953 1107 21 0.41928453265358456 1107 22 0.510713462420469 1107 23 0.424170881704633 1107 24 0.46333828427456325 1107 25 0.49545543340409637 1108 1 0.9783092867408385 1108 2 3.4454184166685984 1108 3 1.951545401304621 1108 4 1.2191466906032389 1108 5 0.6489484321493606 1108 6 0.5096288749061202 1108 7 0.41596425894431377 1108 8 0.33211797467141935 1108 9 0.2882912801321851 1108 10 0.3287910869962687 1108 11 0.43866098830234534 1108 12 0.33433281677408644 1108 13 0.4107386126390935 1108 14 0.4486655328030196 1108 15 0.47836908278833334 1108 16 0.5183651311255559 1108 17 0.3723059307675811 1108 18 0.40443929418510294 1108 19 0.3878326251307058 1108 20 0.5497481017977819 1108 21 0.48123092165884174 1108 22 0.6060569472865396 1108 23 0.5125359388782087 1108 24 0.46601977134264416 1108 25 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-0.2863141109135583 2315 8 -0.2866581627632456 2315 9 -0.2599018660770227 2315 10 -0.30005004067385244 2315 11 -0.43703326875333204 2315 12 -0.5095115458076052 2315 13 -0.42866517855765807 2315 14 -0.442352600644206 2315 15 -0.8670065416340336 2315 16 -0.694387562259358 2315 17 -0.6376672365292393 2315 18 -0.6453195914432683 2315 19 -0.608658049915654 2315 20 -0.32550674203813657 2315 21 -0.4627602520810612 2315 22 -0.8341601629505888 2315 23 -0.7315589842279948 2315 24 -0.5241864854935017 2315 25 -0.4892410921189736 2316 1 -0.4647849611594346 2316 2 0.030700929223609563 2316 3 0.08985041626589663 2316 4 -0.2901278546444601 2316 5 -0.562071444584916 2316 6 -0.658652140618571 2316 7 -0.8087376442164285 2316 8 -1.0440053849607693 2316 9 -1.0045545763082018 2316 10 -1.0617452977026005 2316 11 -1.1039260028834121 2316 12 -1.0436256239306871 2316 13 -1.16679690345714 2316 14 -1.2477063808134545 2316 15 -1.1098652340584039 2316 16 -1.2107912047076468 2316 17 -1.2614612223285988 2316 18 -1.2488322317203853 2316 19 -1.0507293589671889 2316 20 -0.9163675469544827 2316 21 -1.0835113959030025 2316 22 -1.3243934439458087 2316 23 -1.0517003860477985 2316 24 -1.2089863233224065 2316 25 -1.0808764774264787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35639.0 3 0.0 4 313.0 5 1504.0 6 1737.0 7 1586.0 8 1491.0 9 1453.0 10 1400.0 11 1353.0 12 1354.0 13 1336.0 14 1376.0 15 1428.0 16 1545.0 17 1611.0 18 1929.0 19 2318.0 20 2975.0 21 3754.0 22 4731.0 23 5526.0 24 6575.0 25 7535.0 26 8815.0 27 10231.0 28 12183.0 29 15263.0 30 19363.0 31 25809.0 32 35555.0 33 49732.0 34 73102.0 35 112727.0 36 190006.0 37 274174.0 38 680015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.07872075640836 19.014312864801518 13.081460132561768 25.825506246228354 2 16.807832042632267 21.529320679223755 38.95737484791977 22.7054724302242 3 18.16946321748994 24.442922568803663 30.07222788404476 27.315386329661635 4 12.124368678964647 17.02200515323229 38.73337656906909 32.12024959873398 5 13.101429533680054 37.4703588983401 36.13629022363225 13.291921344347596 6 31.13767994371008 37.56582390368614 18.439457032609592 12.857039119994191 7 27.596272545391265 31.588337368946895 22.75309807417349 18.062292011488353 8 24.6937136411127 36.96232040830252 20.956491092326925 17.387474858257853 9 25.907324450810172 15.779019483625092 20.714528960013624 37.59912710555112 10 14.523773305213394 29.093119441507458 34.86296537654239 21.52014187673676 11 33.59124550922944 22.768011127783588 24.980797436903853 18.65994592608312 12 22.816950077749205 24.782214587804017 32.08682119686198 20.314014137584792 13 28.302463184937938 22.166849041343546 26.071586860008868 23.45910091370965 14 20.679110303153227 22.163106915238917 28.075493147486323 29.082289634121533 15 22.547770062675124 30.75024852201392 23.718077128212318 22.98390428709864 16 21.817102821757757 28.323121186094784 27.843104359213843 22.016671632933612 17 21.460157601768827 28.64119180390674 28.090249173053955 21.808401421270478 18 21.31026137493998 27.634391841430993 29.86803235432759 21.187314429301438 19 23.05197914899504 27.08139141082327 27.898829932347542 21.96779950783415 20 23.069984068209347 27.39892389987699 28.47214440650167 21.05894762541199 21 22.61972506383855 26.884497138768324 27.564982605990153 22.930795191402968 22 22.714991498749228 27.981635295089806 27.809231431841003 21.494141774319964 23 21.970156990057752 27.712269613741107 28.132821176066997 22.18475222013414 24 22.522980461286906 26.958503709676208 28.751993810111653 21.766522018925233 25 22.29332218214416 27.360936699890697 28.376625571696678 21.969115546268462 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 160.0 2 315.5 3 471.0 4 471.0 5 471.0 6 1096.5 7 1722.0 8 1722.0 9 1722.0 10 2662.5 11 3603.0 12 3603.0 13 3603.0 14 6072.5 15 8542.0 16 8542.0 17 8542.0 18 14864.5 19 21187.0 20 21187.0 21 21187.0 22 32453.5 23 43720.0 24 43720.0 25 43720.0 26 60871.0 27 78022.0 28 78022.0 29 78022.0 30 99891.5 31 121761.0 32 121761.0 33 121761.0 34 138696.0 35 155631.0 36 155631.0 37 155631.0 38 167729.0 39 179827.0 40 179827.0 41 179827.0 42 188949.0 43 198071.0 44 198071.0 45 198071.0 46 201655.5 47 205240.0 48 205240.0 49 205240.0 50 198690.5 51 192141.0 52 192141.0 53 192141.0 54 171498.0 55 150855.0 56 150855.0 57 150855.0 58 129257.0 59 107659.0 60 107659.0 61 107659.0 62 87243.0 63 66827.0 64 66827.0 65 66827.0 66 51576.5 67 36326.0 68 36326.0 69 36326.0 70 26194.0 71 16062.0 72 16062.0 73 16062.0 74 11205.0 75 6348.0 76 6348.0 77 6348.0 78 4288.0 79 2228.0 80 2228.0 81 2228.0 82 1451.0 83 674.0 84 674.0 85 674.0 86 454.5 87 235.0 88 235.0 89 235.0 90 160.0 91 85.0 92 85.0 93 85.0 94 61.5 95 38.0 96 38.0 97 38.0 98 23.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.216701180135266 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 6.260000350560019E-5 9 0.0 10 6.260000350560019E-5 11 6.260000350560019E-5 12 0.0 13 0.0 14 6.260000350560019E-5 15 0.0 16 0.0 17 0.0 18 4.382000245392014E-4 19 9.390000525840029E-4 20 5.634000315504018E-4 21 0.0034430001928080108 22 0.002003200112179206 23 0.0011268000631008035 24 0.0 25 3.756000210336012E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1597444.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.30104574686555 #Duplication Level Percentage of deduplicated Percentage of total 1 84.83830979610165 64.73251756839105 2 10.850301665270766 16.557787274582324 3 2.477255754580122 5.670516139707115 4 0.806448903680585 2.461315787689675 5 0.35528293442715203 1.3554229716403383 6 0.18380086214008975 0.841451879427859 7 0.10898200837929832 0.582080884485389 8 0.06974817384411132 0.4257486882591895 9 0.05532528447001276 0.3799239355174315 >10 0.22961538307190615 3.0272862387981707 >50 0.013109461050960747 0.7150171754402392 >100 0.00958831136191284 1.431073774638442 >500 0.0015702878076470652 0.8132258449574846 >1k 6.611738137461327E-4 1.0066318364652858 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2998 0.18767481050978937 No Hit GTCCTACAGTGGACATTTCTAAATT 2367 0.14817420829775566 No Hit CTGTAGGACGTGGAATATGGCAAGA 2170 0.13584200760715243 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1970 0.12332200690603239 No Hit GGTATCAACGCAGAGTACTTTTTTT 1845 0.11549700646783237 No Hit TATCAACGCAGAGTACTTTTTTTTT 1738 0.10879880609273314 No Hit CTTTAGGACGTGAAATATGGCGAGG 1697 0.10623220594900354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 6.260000350560019E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 60 3.1953614E-4 13.043208 1 GGTATCA 760 0.0 11.713145 1 GGCGAGG 610 0.0 11.641263 19 TAGGACC 420 0.0 11.496413 4 ACACCGT 75 2.1406563E-4 11.361162 6 GGACGTG 1350 0.0 11.220901 6 AGGACGT 1360 0.0 11.138394 5 GTAGGAC 1300 0.0 10.997023 3 TAGGACG 1430 0.0 10.857987 4 TGTAGGA 1330 0.0 10.812133 2 TGGCGAG 1250 0.0 10.755907 18 GACGTGA 755 0.0 10.658927 7 CTGTAGG 1280 0.0 10.623081 1 GACGTGG 685 0.0 10.504238 7 GCGCCAC 100 2.489657E-5 10.414724 13 CGTGCGC 100 2.4905168E-5 10.414399 10 TTAGGAC 925 0.0 10.235281 3 ATTTCTC 1545 0.0 10.1113825 15 CCCGTTT 75 0.002722943 10.099127 12 CTACACG 75 0.00272365 10.09881 4 >>END_MODULE