FastQCFastQC Report
Thu 26 May 2016
SRR1512018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597444
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39640.24814641389619918No Hit
TATCAACGCAGAGTACTTTTTTTTT25540.1598804089533029No Hit
GGTATCAACGCAGAGTACTTTTTTT25300.1583780088691685No Hit
CTGTAGGACGTGGAATATGGCAAGA22440.14047440786656684No Hit
GTCCTACAGTGGACATTTCTAAATT21730.13602980761766922No Hit
GTCCTAAAGTGTGTATTTCTCATTT20010.125262607014706No Hit
CTTTAGGACGTGAAATATGGCGAGG19510.12213260683942599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG551.9547847E-413.8226935
GGTATCA10900.013.5684151
ACCGACT600.00589577511.0782768
ATCGTGC600.00589577511.0782768
AAGGCGT1303.26163E-810.9623696
TACACGG700.001487385710.8606875
TGGTATC3350.010.7796372
GACGTGG5950.010.6925977
GTGCTAG1355.3318217E-810.6018431
TGTAGGA13300.010.50342752
GGACGTG11950.010.3355126
AGGACGT12000.010.2158345
CTGTAGG13500.010.107091
GCGTGCA856.6208787E-410.0542349
GTACTAG1053.9116912E-59.9960231
CGTGCGC951.6514759E-49.99543710
TAGGACC4000.09.9782564
AATGCCG1054.1222014E-59.94846218
GTGGTAT3550.09.9448271
GTAGGAC13250.09.897573
Produced by FastQC (version 0.11.5)